Pathogenic and enzyme activities of the entomopathogenic fungus <i>Tolypocladium cylindrosporum</i> (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina

Tolypocladium cylindrosporum is an entomopathogenic fungi that has been studied as a biological control agent against insects of several orders. The fungus has been isolated from the soil as well as from insects of the orders Coleoptera, Lepidoptera, Diptera and Hymenoptera. In this study, we analyz...

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Detalles Bibliográficos
Autores principales: Scorsetti, Ana Clara, Elíades, Lorena Alejandra, Stenglein, Sebastián Alberto, Cabello, Marta Noemí, Pelizza, Sebastián Alberto, Saparrat, Mario Carlos Nazareno
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Agrarias
Hongos
biological control, chitinolytic activity, entomopathogenesis, hydrolases, soil microorganisms, Tolypocladium cylindrosporum
Ascomicetos
Insectos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/66619
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-66619
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Hongos
biological control, chitinolytic activity, entomopathogenesis, hydrolases, soil microorganisms, Tolypocladium cylindrosporum
Ascomicetos
Insectos
spellingShingle Ciencias Agrarias
Hongos
biological control, chitinolytic activity, entomopathogenesis, hydrolases, soil microorganisms, Tolypocladium cylindrosporum
Ascomicetos
Insectos
Scorsetti, Ana Clara
Elíades, Lorena Alejandra
Stenglein, Sebastián Alberto
Cabello, Marta Noemí
Pelizza, Sebastián Alberto
Saparrat, Mario Carlos Nazareno
Pathogenic and enzyme activities of the entomopathogenic fungus <i>Tolypocladium cylindrosporum</i> (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina
topic_facet Ciencias Agrarias
Hongos
biological control, chitinolytic activity, entomopathogenesis, hydrolases, soil microorganisms, Tolypocladium cylindrosporum
Ascomicetos
Insectos
description Tolypocladium cylindrosporum is an entomopathogenic fungi that has been studied as a biological control agent against insects of several orders. The fungus has been isolated from the soil as well as from insects of the orders Coleoptera, Lepidoptera, Diptera and Hymenoptera. In this study, we analyzed the ability of a strain of T. cylindrosporum, isolated from soil samples taken in Tierra del Fuego, Argentina, to produce hydrolytic enzymes, and to study the relationship of those activities to the fungus pathogenicity against pest aphids. We have made the traditional and molecular characterization of this strain of T. cylindrosporum. The expression of hydrolase activity in the fungal strain was estimated at three incubation temperatures (4ºC, 12ºC and 24ºC), on different agar media supplemented with the following specific substrates: chitin azure, Tween ® 20, casein, and urea for chitinase, lipase, protease, and urease activity, respectively. The hydrolytic-enzyme activity was estimated qualitatively according to the presence of a halo of clarification through hydrolase action, besides was expressed semi-quantitatively as the ratio between the hydrolytic-halo and colony diameters. The pathogenicity of the fungus was tested on adults of the aphid Rhopalosiphum padi at three temperatures of incubation (4ºC, 12ºC and 24ºC). The suspension was adjusted to a concentration of 1x10 7 conidia/ml. In pathogenicity assays at seven days post-inoculation, the fungus caused the mortality of adults of Ropalosiphum padi at different temperatures also showed a broad ability to grow on several agar-culture media, supplemented with different carbon sources at the three incubation temperatures tested. Although, the growth was greater with higher incubation temperatures (with maximum levels at 24°C), the fungus reached similar colony diameters after 15 days of incubation on the medium supplemented with Tween® 20 at the lower two incubation temperatures of 4°C or 12°C. In accordance with the results on colony diameters, the fungus revealed an ability to degrade casein, chitin derivatives, Tween® 20, and urea as evidenced by the appearance of a halo around the fungal colony. Because of its origin and temperature tolerance, this Argentine strain has great potential for use as a biocontrol agent for insect pest control in cold and temperate environments.
format Articulo
Articulo
author Scorsetti, Ana Clara
Elíades, Lorena Alejandra
Stenglein, Sebastián Alberto
Cabello, Marta Noemí
Pelizza, Sebastián Alberto
Saparrat, Mario Carlos Nazareno
author_facet Scorsetti, Ana Clara
Elíades, Lorena Alejandra
Stenglein, Sebastián Alberto
Cabello, Marta Noemí
Pelizza, Sebastián Alberto
Saparrat, Mario Carlos Nazareno
author_sort Scorsetti, Ana Clara
title Pathogenic and enzyme activities of the entomopathogenic fungus <i>Tolypocladium cylindrosporum</i> (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina
title_short Pathogenic and enzyme activities of the entomopathogenic fungus <i>Tolypocladium cylindrosporum</i> (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina
title_full Pathogenic and enzyme activities of the entomopathogenic fungus <i>Tolypocladium cylindrosporum</i> (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina
title_fullStr Pathogenic and enzyme activities of the entomopathogenic fungus <i>Tolypocladium cylindrosporum</i> (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina
title_full_unstemmed Pathogenic and enzyme activities of the entomopathogenic fungus <i>Tolypocladium cylindrosporum</i> (Ascomycota: Hypocreales) from Tierra del Fuego, Argentina
title_sort pathogenic and enzyme activities of the entomopathogenic fungus <i>tolypocladium cylindrosporum</i> (ascomycota: hypocreales) from tierra del fuego, argentina
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/66619
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