Dicistroviridae: A new viral purification technique

The family Dicistroviridae comprises three genera and about twenty species of RNA virus, most of them with health or agricultural importance. The Triatoma virus (TrV) is the only entomopathogenic virus identified in triatomine bugs up to the present. TrV replicates within the intestinal epithelial c...

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Detalles Bibliográficos
Autores principales:	Susevich, María Laura, Metz, Germán Ernesto, Martí, Gerardo Aníbal, Echeverría, María Gabriela
Formato:	Articulo Comunicacion
Lenguaje:	Inglés
Publicado:	2017
Materias:	Ciencias Veterinarias Triatoma ARN Viral
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/66368 https://www.sciencedirect.com/science/article/pii/S0325754117300834
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-66368
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Veterinarias Triatoma ARN Viral
spellingShingle	Ciencias Veterinarias Triatoma ARN Viral Susevich, María Laura Metz, Germán Ernesto Martí, Gerardo Aníbal Echeverría, María Gabriela Dicistroviridae: A new viral purification technique
topic_facet	Ciencias Veterinarias Triatoma ARN Viral
description	The family Dicistroviridae comprises three genera and about twenty species of RNA virus, most of them with health or agricultural importance. The Triatoma virus (TrV) is the only entomopathogenic virus identified in triatomine bugs up to the present. TrV replicates within the intestinal epithelial cells, causing high mortality rate and delayed development of the molt of these bugs. TrV has been proposed as a biological control agent for vectors of Chagas disease. Viral particles were purified from feces of 1, 5 and 10 insects from an experimental colony of Triatoma infestans infected with TrV. Viral concentration and infectivity were corroborated using polyacrylamide gels and RT-PCR, respectively. In this work we report a method of viral purification that allows to reduce necessary reagents and time, using a very small amount of fecal matter.
format	Articulo Comunicacion
author	Susevich, María Laura Metz, Germán Ernesto Martí, Gerardo Aníbal Echeverría, María Gabriela
author_facet	Susevich, María Laura Metz, Germán Ernesto Martí, Gerardo Aníbal Echeverría, María Gabriela
author_sort	Susevich, María Laura
title	Dicistroviridae: A new viral purification technique
title_short	Dicistroviridae: A new viral purification technique
title_full	Dicistroviridae: A new viral purification technique
title_fullStr	Dicistroviridae: A new viral purification technique
title_full_unstemmed	Dicistroviridae: A new viral purification technique
title_sort	dicistroviridae: a new viral purification technique
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/66368 https://www.sciencedirect.com/science/article/pii/S0325754117300834
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bdutipo_str	Repositorios
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Dicistroviridae: A new viral purification technique

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