Complete genome amplification of Equine influenza virus subtype 2

This work reports a method for rapid amplification of the complete genome of equine influenza virus subtype 2 (H3N8). A ThermoScriptTM reverse transcriptase instead of the avian myeloblastosis virus reverse transcriptase or Moloney murine leukemia virus reverse transcriptase was used. This enzyme ha...

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Detalles Bibliográficos
Autores principales: Sguazza, Guillermo Hernán, Fuentealba, Nadia Analía, Tizzano, Marco Antonio, Galosi, Cecilia Mónica, Pecoraro, Marcelo Ricardo Ítalo
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2009
Materias:
Ciencias Veterinarias
Virología
virus de la influenza equina, genoma, diagnóstico, RT-PCR
Enfermedades de los Caballos
Equine influenza virus, genome, diagnosis, RT-PCR
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/66350
http://www.scielo.org.ar/pdf/ram/v41n4/v41n4a02.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-66350
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Virología
virus de la influenza equina, genoma, diagnóstico, RT-PCR
Enfermedades de los Caballos
Equine influenza virus, genome, diagnosis, RT-PCR
spellingShingle Ciencias Veterinarias
Virología
virus de la influenza equina, genoma, diagnóstico, RT-PCR
Enfermedades de los Caballos
Equine influenza virus, genome, diagnosis, RT-PCR
Sguazza, Guillermo Hernán
Fuentealba, Nadia Analía
Tizzano, Marco Antonio
Galosi, Cecilia Mónica
Pecoraro, Marcelo Ricardo Ítalo
Complete genome amplification of Equine influenza virus subtype 2
topic_facet Ciencias Veterinarias
Virología
virus de la influenza equina, genoma, diagnóstico, RT-PCR
Enfermedades de los Caballos
Equine influenza virus, genome, diagnosis, RT-PCR
description This work reports a method for rapid amplification of the complete genome of equine influenza virus subtype 2 (H3N8). A ThermoScriptTM reverse transcriptase instead of the avian myeloblastosis virus reverse transcriptase or Moloney murine leukemia virus reverse transcriptase was used. This enzyme has demonstrated higher thermal stability and is described as suitable to make long cDNA with a complex secondary structure. The product obtained by this method can be cloned, used in later sequencing reactions or nested-PCR with the purpose of achieving a rapid diagnosis and characterization of the equine influenza virus type A. This detection assay might be a valuable tool for diagnosis and screening of field samples as well as for conducting molecular studies.
format Articulo
Comunicacion
author Sguazza, Guillermo Hernán
Fuentealba, Nadia Analía
Tizzano, Marco Antonio
Galosi, Cecilia Mónica
Pecoraro, Marcelo Ricardo Ítalo
author_facet Sguazza, Guillermo Hernán
Fuentealba, Nadia Analía
Tizzano, Marco Antonio
Galosi, Cecilia Mónica
Pecoraro, Marcelo Ricardo Ítalo
author_sort Sguazza, Guillermo Hernán
title Complete genome amplification of Equine influenza virus subtype 2
title_short Complete genome amplification of Equine influenza virus subtype 2
title_full Complete genome amplification of Equine influenza virus subtype 2
title_fullStr Complete genome amplification of Equine influenza virus subtype 2
title_full_unstemmed Complete genome amplification of Equine influenza virus subtype 2
title_sort complete genome amplification of equine influenza virus subtype 2
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/66350
http://www.scielo.org.ar/pdf/ram/v41n4/v41n4a02.pdf
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