Evaluation of western blotting for the diagnosis of enzootic bovine leukemia

A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally BLV infected cows were used. An agar gel immu...

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Autores principales: González, Ester Teresa, Oliva, Graciela A., Norimine, Junzo, Cid de la Paz, Viviana, Echeverría, María Gabriela
Formato: Articulo
Lenguaje:Inglés
Publicado: 1999
Materias:
Ciencias Veterinarias
Bovine leukosis
Diagnosis
Western blotting
Leucose bovina
diagnóstico
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/58634
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-58634
record_format dspace
spelling I19-R120-10915-586342023-09-22T16:01:25Z http://sedici.unlp.edu.ar/handle/10915/58634 Evaluation of western blotting for the diagnosis of enzootic bovine leukemia González, Ester Teresa Oliva, Graciela A. Norimine, Junzo Cid de la Paz, Viviana Echeverría, María Gabriela 1999 2017-02-10T17:29:22Z en Ciencias Veterinarias Bovine leukosis Diagnosis Western blotting Leucose bovina diagnóstico A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally BLV infected cows were used. An agar gel immunodiffusion test (AGID) was used for comparing with the results obtained by WB. The AGID positive sera showed a different degree of reactivity by WB test against the two most important viral antigens (gp51 and p24), or against one of them. Other proteins (gp30, p15, p12 and p10) were not detected with any AGID positive sera, being observed occasionally three bands corresponding to the p24 protein. Using sera obtained by BLV experimental inoculation, the antibodies directed to p24 appeared early (between the 2nd and 4th week post inoculation) and thereafter antibodies to gp51 were detected in some animals. The analysis of field serum samples by AGID as compared to WB showed an agreement of 90.9%. Only 1.7% of sera were negative by AGID and positive by WB and 7.2% that were not conclusive by AGID and were defined by WB (4.2% as positive and 3.0% as negative). Um sistema de western blotting (WB) foi desenvolvido para detecção de anticorpos contra o vírus da leucose em soros de bovinos. Foram utilizadas amostras de soros de 233 animais naturalmente infectados e soros de vacas experimentalmente infectadas. O teste de imunodifusão em ágar (AGID) foi usado para comparação dos resultados. Graus diferentes de reatividade foram observados em soros positivos ao AGID, quando testados em WB frente a um ou aos dois antígenos mais importantes (gp51 e p24). Outras proteínas (gp30, p15, p12 e p10) não foram detectadas por nenhum soro positivo ao AGID, sendo que três bandas correspondentes à proteína p24 foram observadas ocasionalmente. Em soros obtidos por inoculação experimental, anticorpos contra a proteína p24 foram detectados entre a segunda e a quarta semanas após a inoculação e, em alguns animais, detectaram-se anticorpos anti-gp51 mais tardiamente. O estudo de soros de campo com AGID e WB mostrou concordância de 90,9% sendo que apenas 1,7% dos soros negativos pelo AGID foram positivos ao WB e 7,2% dos resultados não conclusivos por AGID foram definidos por WB (4,2% como positivos e 3% como negativos). Facultad de Ciencias Veterinarias Articulo Articulo http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Bovine leukosis
Diagnosis
Western blotting
Leucose bovina
diagnóstico
spellingShingle Ciencias Veterinarias
Bovine leukosis
Diagnosis
Western blotting
Leucose bovina
diagnóstico
González, Ester Teresa
Oliva, Graciela A.
Norimine, Junzo
Cid de la Paz, Viviana
Echeverría, María Gabriela
Evaluation of western blotting for the diagnosis of enzootic bovine leukemia
topic_facet Ciencias Veterinarias
Bovine leukosis
Diagnosis
Western blotting
Leucose bovina
diagnóstico
description A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally BLV infected cows were used. An agar gel immunodiffusion test (AGID) was used for comparing with the results obtained by WB. The AGID positive sera showed a different degree of reactivity by WB test against the two most important viral antigens (gp51 and p24), or against one of them. Other proteins (gp30, p15, p12 and p10) were not detected with any AGID positive sera, being observed occasionally three bands corresponding to the p24 protein. Using sera obtained by BLV experimental inoculation, the antibodies directed to p24 appeared early (between the 2nd and 4th week post inoculation) and thereafter antibodies to gp51 were detected in some animals. The analysis of field serum samples by AGID as compared to WB showed an agreement of 90.9%. Only 1.7% of sera were negative by AGID and positive by WB and 7.2% that were not conclusive by AGID and were defined by WB (4.2% as positive and 3.0% as negative).
format Articulo
Articulo
author González, Ester Teresa
Oliva, Graciela A.
Norimine, Junzo
Cid de la Paz, Viviana
Echeverría, María Gabriela
author_facet González, Ester Teresa
Oliva, Graciela A.
Norimine, Junzo
Cid de la Paz, Viviana
Echeverría, María Gabriela
author_sort González, Ester Teresa
title Evaluation of western blotting for the diagnosis of enzootic bovine leukemia
title_short Evaluation of western blotting for the diagnosis of enzootic bovine leukemia
title_full Evaluation of western blotting for the diagnosis of enzootic bovine leukemia
title_fullStr Evaluation of western blotting for the diagnosis of enzootic bovine leukemia
title_full_unstemmed Evaluation of western blotting for the diagnosis of enzootic bovine leukemia
title_sort evaluation of western blotting for the diagnosis of enzootic bovine leukemia
publishDate 1999
url http://sedici.unlp.edu.ar/handle/10915/58634
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