Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress
<i>Microcystis</i> are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a nativeMicrocystis aeruginosa, we exposed the cells...
| Autores principales: | , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/56853 http://www.sciencedirect.com/science/article/pii/S1532045616300734 |
| Aporte de: |
| Sumario: | <i>Microcystis</i> are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a nativeMicrocystis aeruginosa, we exposed the cells to low(23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth ratewas significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidencewith the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell−1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell−1 on day 2 and 70 fg.cell−1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production. |
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