Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress
<i>Microcystis</i> are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a nativeMicrocystis aeruginosa, we exposed the cells...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2016
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/56853 http://www.sciencedirect.com/science/article/pii/S1532045616300734 |
| Aporte de: |
| id |
I19-R120-10915-56853 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Biología catalase Chlorophyll Microcystin variants ROS Toxinas Biológicas |
| spellingShingle |
Ciencias Exactas Biología catalase Chlorophyll Microcystin variants ROS Toxinas Biológicas Giannuzzi, Leda Krock, Bernd Crettaz Minaglia, Melina Celeste Rosso, Lorena Houghton, Christian Sedan, Daniela Malanga, Gabriela Espinosa, Mariela Andrinolo, Darío Hernando, Marcelo Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| topic_facet |
Ciencias Exactas Biología catalase Chlorophyll Microcystin variants ROS Toxinas Biológicas |
| description |
<i>Microcystis</i> are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a nativeMicrocystis aeruginosa, we exposed the cells to low(23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth ratewas significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidencewith the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell−1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell−1 on day 2 and 70 fg.cell−1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production. |
| format |
Articulo Articulo |
| author |
Giannuzzi, Leda Krock, Bernd Crettaz Minaglia, Melina Celeste Rosso, Lorena Houghton, Christian Sedan, Daniela Malanga, Gabriela Espinosa, Mariela Andrinolo, Darío Hernando, Marcelo |
| author_facet |
Giannuzzi, Leda Krock, Bernd Crettaz Minaglia, Melina Celeste Rosso, Lorena Houghton, Christian Sedan, Daniela Malanga, Gabriela Espinosa, Mariela Andrinolo, Darío Hernando, Marcelo |
| author_sort |
Giannuzzi, Leda |
| title |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title_short |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title_full |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title_fullStr |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title_full_unstemmed |
Growth, toxin production, active oxygen species and catalase activity of <i>Microcystis aeruginosa</i> (Cyanophyceae) exposed to temperature stress |
| title_sort |
growth, toxin production, active oxygen species and catalase activity of <i>microcystis aeruginosa</i> (cyanophyceae) exposed to temperature stress |
| publishDate |
2016 |
| url |
http://sedici.unlp.edu.ar/handle/10915/56853 http://www.sciencedirect.com/science/article/pii/S1532045616300734 |
| work_keys_str_mv |
AT giannuzzileda growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress AT krockbernd growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress AT crettazminagliamelinaceleste growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress AT rossolorena growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress AT houghtonchristian growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress AT sedandaniela growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress AT malangagabriela growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress AT espinosamariela growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress AT andrinolodario growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress AT hernandomarcelo growthtoxinproductionactiveoxygenspeciesandcatalaseactivityofimicrocystisaeruginosaicyanophyceaeexposedtotemperaturestress |
| bdutipo_str |
Repositorios |
| _version_ |
1764820476674179073 |