Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions

The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfu...

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Detalles Bibliográficos
Autores principales:	López, Silvina Marianela Yanil, Balatti, Pedro Alberto
Formato:	Articulo
Lenguaje:	Español
Publicado:	2012
Materias:	Ciencias Agrarias inoculant bacterial DNA article molecular markers rhizobia artifact bacterial strain bacterium culture bacterium identification bacterium isolate bacterium mutant Bradyrhizobium Bradyrhizobium elkanii Bradyrhizobium japonicum controlled study DNA fingerprinting gene amplification inoculation nonhuman polymerase chain reaction soybean Bacteria (microorganisms) Bradyrhizobiaceae Glycine max
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/40776 http://astonjournals.com/manuscripts/Vol2011/GEBJ-34_Vol2011.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

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institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Español
topic	Ciencias Agrarias inoculant bacterial DNA article molecular markers rhizobia artifact bacterial strain bacterium culture bacterium identification bacterium isolate bacterium mutant Bradyrhizobium Bradyrhizobium elkanii Bradyrhizobium japonicum controlled study DNA fingerprinting gene amplification inoculation nonhuman polymerase chain reaction soybean Bacteria (microorganisms) Bradyrhizobiaceae Glycine max
spellingShingle	Ciencias Agrarias inoculant bacterial DNA article molecular markers rhizobia artifact bacterial strain bacterium culture bacterium identification bacterium isolate bacterium mutant Bradyrhizobium Bradyrhizobium elkanii Bradyrhizobium japonicum controlled study DNA fingerprinting gene amplification inoculation nonhuman polymerase chain reaction soybean Bacteria (microorganisms) Bradyrhizobiaceae Glycine max López, Silvina Marianela Yanil Balatti, Pedro Alberto Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
topic_facet	Ciencias Agrarias inoculant bacterial DNA article molecular markers rhizobia artifact bacterial strain bacterium culture bacterium identification bacterium isolate bacterium mutant Bradyrhizobium Bradyrhizobium elkanii Bradyrhizobium japonicum controlled study DNA fingerprinting gene amplification inoculation nonhuman polymerase chain reaction soybean Bacteria (microorganisms) Bradyrhizobiaceae Glycine max
description	The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
format	Articulo Articulo
author	López, Silvina Marianela Yanil Balatti, Pedro Alberto
author_facet	López, Silvina Marianela Yanil Balatti, Pedro Alberto
author_sort	López, Silvina Marianela Yanil
title	Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_short	Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_full	Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_fullStr	Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_full_unstemmed	Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_sort	closely related strains of <i>bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of pcr reactions
publishDate	2012
url	http://sedici.unlp.edu.ar/handle/10915/40776 http://astonjournals.com/manuscripts/Vol2011/GEBJ-34_Vol2011.pdf
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bdutipo_str	Repositorios
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Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions

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