Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi
Background: Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region - which extends through northern A...
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| Autores principales: | , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2009
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/40293 http://www.plosntds.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pntd.0000434&representation=PDF |
| Aporte de: |
| id |
I19-R120-10915-40293 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Médicas insecticide Chagas disease insect microbiology parasitology physiology |
| spellingShingle |
Ciencias Médicas insecticide Chagas disease insect microbiology parasitology physiology Pedrini, Nicolás Mijailovsky, Sergio Javier Girotti, Juan Roberto Stariolo, Raúl Cardozo, Rubén M. Gentile, Alberto Juárez, Marta Patricia Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi |
| topic_facet |
Ciencias Médicas insecticide Chagas disease insect microbiology parasitology physiology |
| description |
Background: Triatoma infestans-mediated transmission of Tripanosoma cruzi, the causative agent of Chagas disease, remains as a major health issue in southern South America. Key factors of T. infestans prevalence in specific areas of the geographic Gran Chaco region - which extends through northern Argentina, Bolivia, and Paraguay - are both recurrent reinfestations after insecticide spraying and emerging pyrethroid-resistance over the past ten years. Among alternative control tools, the pathogenicity of entomopathogenic fungi against triatomines is already known; furthermore, these fungi have the ability to fully degrade hydrocarbons from T. infestans cuticle and to utilize them as fuel and for incorporation into cellular components. Methodology and Findings: Here we provide evidence of resistance-related cuticle differences; capillary gas chromatography coupled to mass spectrometry analyses revealed that pyrethroid-resistant bugs have significantly larger amounts of surface hydrocarbons, peaking 56.2±6.4% higher than susceptible specimens. Also, a thicker cuticle was detected by scanning electron microscopy (32.1±5.9 μm and 17.8±5.4 μm for pyrethroid-resistant and pyrethroid-susceptible, respectively). In laboratory bioassays, we showed that the virulence of the entomopathogenic fungi Beauveria bassiana against T. infestans was significantly enhanced after fungal adaptation to grow on a medium containing insect-like hydrocarbons as the carbon source, regardless of bug susceptibility to pyrethroids. We designed an attraction-infection trap based on manipulating T. infestans behavior in order to facilitate close contact with B. bassiana. Field assays performed in rural village houses infested with pyrethroid-resistant insects showed 52.4% bug mortality. Using available mathematical models, we predicted that further fungal applications could eventually halt infection transmission. Conclusions: This low cost, low tech, ecologically friendly methodology could help in controlling the spread of pyrethroid-resistant bugs. |
| format |
Articulo Articulo |
| author |
Pedrini, Nicolás Mijailovsky, Sergio Javier Girotti, Juan Roberto Stariolo, Raúl Cardozo, Rubén M. Gentile, Alberto Juárez, Marta Patricia |
| author_facet |
Pedrini, Nicolás Mijailovsky, Sergio Javier Girotti, Juan Roberto Stariolo, Raúl Cardozo, Rubén M. Gentile, Alberto Juárez, Marta Patricia |
| author_sort |
Pedrini, Nicolás |
| title |
Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi |
| title_short |
Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi |
| title_full |
Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi |
| title_fullStr |
Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi |
| title_full_unstemmed |
Control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi |
| title_sort |
control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi |
| publishDate |
2009 |
| url |
http://sedici.unlp.edu.ar/handle/10915/40293 http://www.plosntds.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pntd.0000434&representation=PDF |
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AT pedrininicolas controlofpyrethroidresistantchagasdiseasevectorswithentomopathogenicfungi AT mijailovskysergiojavier controlofpyrethroidresistantchagasdiseasevectorswithentomopathogenicfungi AT girottijuanroberto controlofpyrethroidresistantchagasdiseasevectorswithentomopathogenicfungi AT starioloraul controlofpyrethroidresistantchagasdiseasevectorswithentomopathogenicfungi AT cardozorubenm controlofpyrethroidresistantchagasdiseasevectorswithentomopathogenicfungi AT gentilealberto controlofpyrethroidresistantchagasdiseasevectorswithentomopathogenicfungi AT juarezmartapatricia controlofpyrethroidresistantchagasdiseasevectorswithentomopathogenicfungi |
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