Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)

In cancer patients, MUC1 glycoprotein may carry Lewis y which could be involved in immune response. Purposes: 1- to evaluate the presence of Lewis y and MUC1 in circulating immune complexes (Lewis y/CIC and MUC1/CIC, respectively) and their correlation; 2- to analyze the possible presence of Lewis y...

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Autores principales:	Isla Larrain, Marina Teresita, Demichelis, Sandra O., Crespo, Marina, Lacunza, Ezequiel, Barbera, Alberto, Cretón, Aldo, Terrier, Francisco, Segal-Eiras, Amada, Croce, María Virginia
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2009
Materias:	Ciencias Médicas antigen antibody complex breast tumor cancer staging immunology pathology
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/37727 http://www.jeccr.com/content/pdf/1756-9966-28-121.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-37727
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas antigen antibody complex breast tumor cancer staging immunology pathology
spellingShingle	Ciencias Médicas antigen antibody complex breast tumor cancer staging immunology pathology Isla Larrain, Marina Teresita Demichelis, Sandra O. Crespo, Marina Lacunza, Ezequiel Barbera, Alberto Cretón, Aldo Terrier, Francisco Segal-Eiras, Amada Croce, María Virginia Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)
topic_facet	Ciencias Médicas antigen antibody complex breast tumor cancer staging immunology pathology
description	In cancer patients, MUC1 glycoprotein may carry Lewis y which could be involved in immune response. Purposes: 1- to evaluate the presence of Lewis y and MUC1 in circulating immune complexes (Lewis y/CIC and MUC1/CIC, respectively) and their correlation; 2- to analyze the possible presence of Lewis y in carbohydrate chains of tumoral MUC1 glycoprotein and 3- to correlate serum and tissue parameters considered. Pretreatment serum and tissue breast samples from 76 adenocarcinoma, 34 benign and 36 normal specimens were analyzed. Anti-MUC1 and anti-Lewis y MAbs were employed. To detect Lewis y/CIC and MUC1/CIC, ELISA tests were developed; serum samples containing MUC1 were previously selected by Cancer Associated Serum Antigen (CASA). Immunoprecipitation (IP) was performed in 9 malignant, benign and normal samples and analyzed by SDS-PAGE and Western blot. Lewis y and MUC1 expression was studied by immunohistochemistry (IHC). Statistical analysis was performed employing principal component analysis (PCA), ANOVA, Tukey HSD, Chi square test and classical correlation (p < 0.05). By ELISA, Lewis y/IgM/CIC levels showed statistically significant differences between breast cancer versus benign and normal samples; mean +/- SD values expressed in OD units were: 0.525 +/- 0.304; 0.968 +/- 0.482 and 0.928 +/- 0.447, for breast cancer, benign disease and normal samples, respectively, p < 0.05. Lewis y/IgG/CIC did not show any statistically significant difference. MUC1/IgM/CIC correlated with Lewis y/IgM/CIC. By CASA, 9 samples with MUC1 values above the cut off were selected and IP was performed, followed by SDS-PAGE and Western blot; bands at 200 kDa were obtained with each MAb in all the samples. By IHC, with C14 MAb, 47.5%, 31% and 35% of malignant, benign and normal samples, respectively, showed positive reaction while all the samples were positive with anti-MUC1 MAb; in both cases, with a different pattern of expression between malignant and non malignant samples. Our findings support that in breast cancer there was a limited humoral immune response through Lewis y/IgM/CIC levels detection which correlated with MUC1/IgM/CIC. We also found that Lewis y might be part of circulating MUC1 glycoform structure and also that Lewis y/CIC did not correlate with Lewis y expression.
format	Articulo Articulo
author	Isla Larrain, Marina Teresita Demichelis, Sandra O. Crespo, Marina Lacunza, Ezequiel Barbera, Alberto Cretón, Aldo Terrier, Francisco Segal-Eiras, Amada Croce, María Virginia
author_facet	Isla Larrain, Marina Teresita Demichelis, Sandra O. Crespo, Marina Lacunza, Ezequiel Barbera, Alberto Cretón, Aldo Terrier, Francisco Segal-Eiras, Amada Croce, María Virginia
author_sort	Isla Larrain, Marina Teresita
title	Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)
title_short	Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)
title_full	Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)
title_fullStr	Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)
title_full_unstemmed	Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)
title_sort	breast cancer humoral immune response: involvement of lewis y through the detection of circulating immune complexes and association with mucin 1 (muc1)
publishDate	2009
url	http://sedici.unlp.edu.ar/handle/10915/37727 http://www.jeccr.com/content/pdf/1756-9966-28-121.pdf
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Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)

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