In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis

The aim of the present paper was to evaluate the larvicidal effect of Enterococcus faecalis CECT7121 (Ef7121) on the Toxocara canis cycle both in vitro and in vivo. For the in vitro experiments, T. canis larvae were incubated with the supernat...

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Autores principales:	Chiodo, Paula G., Sparo, Mónica, Pezzani, Betina Cecilia, Minvielle, Marta Cecilia, Basualdo Farjat, Juan Ángel
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Ciencias Médicas anthelmintic agent biological cycle probiotic agent Enterococcus faecalis CECT7121 probiotics bacterial count Toxocara canis chemistry drug effect genetics
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/37459 http://www.scielo.br/pdf/mioc/v105n5/03.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-37459
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas anthelmintic agent biological cycle probiotic agent Enterococcus faecalis CECT7121 probiotics bacterial count Toxocara canis chemistry drug effect genetics
spellingShingle	Ciencias Médicas anthelmintic agent biological cycle probiotic agent Enterococcus faecalis CECT7121 probiotics bacterial count Toxocara canis chemistry drug effect genetics Chiodo, Paula G. Sparo, Mónica Pezzani, Betina Cecilia Minvielle, Marta Cecilia Basualdo Farjat, Juan Ángel <i>In vitro</i> and <i>in vivo</i> effects of <i>Enterococcus faecalis</i> CECT7121 on <i>Toxocara canis</i>
topic_facet	Ciencias Médicas anthelmintic agent biological cycle probiotic agent Enterococcus faecalis CECT7121 probiotics bacterial count Toxocara canis chemistry drug effect genetics
description	The aim of the present paper was to evaluate the larvicidal effect of <i>Enterococcus faecalis</i> CECT7121 (Ef7121) on the <i>Toxocara canis</i> cycle both in vitro and in vivo. For the in vitro experiments, <i>T. canis</i> larvae were incubated with the supernatants of <i>Ef</i>7121 (EI) and mutant <i>Ef</i>7121 (EIm), in a pre-culture of <i>Ef</i>7121 (EII) and in a fresh culture with <i>Ef</i>7121 (EIII) and the <i>Ef</i>7121 mutant strain (EIIIm). The viability of the larvae was calculated after a 48 h incubation. A significant reduction of the viability of <i>T. canis</i> larvae was observed in EI, EII and EIII. A decrease of this inhibitory effect was observed in EIm and EIIIm (p = 0.008). In the in vivo experiments, mice were orally inoculated with three doses of <i>Ef</i>7121. To study the probiotic persistence in the intestine, the animals were sacrificed every four days and their intestines were dissected. The initial average bacterial levels were 9.7 × 10 4 for <i>Ef</i>7121 (colony forming units/g). At the end of the assay the levels were 1.46 × 10 4. No bacterial translocation was detected in mesenteric lymphatic nodules and spleen. <i>Ef</i>7121 interference with the biological cycle was evaluated in mice challenged with <i>T. canis</i>. The interference was significant when the mice were challenged with probiotic and <i>T. canis</i> simultaneously (p = 0.001), but it was not significant when the challenge was performed 15 days after administration of the bacterial inoculum (p = 0.06). In conclusion, <i>Ef</i>7121 possessed in vitro and in vivo larvicidal activity.
format	Articulo Articulo
author	Chiodo, Paula G. Sparo, Mónica Pezzani, Betina Cecilia Minvielle, Marta Cecilia Basualdo Farjat, Juan Ángel
author_facet	Chiodo, Paula G. Sparo, Mónica Pezzani, Betina Cecilia Minvielle, Marta Cecilia Basualdo Farjat, Juan Ángel
author_sort	Chiodo, Paula G.
title	<i>In vitro</i> and <i>in vivo</i> effects of <i>Enterococcus faecalis</i> CECT7121 on <i>Toxocara canis</i>
title_short	<i>In vitro</i> and <i>in vivo</i> effects of <i>Enterococcus faecalis</i> CECT7121 on <i>Toxocara canis</i>
title_full	<i>In vitro</i> and <i>in vivo</i> effects of <i>Enterococcus faecalis</i> CECT7121 on <i>Toxocara canis</i>
title_fullStr	<i>In vitro</i> and <i>in vivo</i> effects of <i>Enterococcus faecalis</i> CECT7121 on <i>Toxocara canis</i>
title_full_unstemmed	<i>In vitro</i> and <i>in vivo</i> effects of <i>Enterococcus faecalis</i> CECT7121 on <i>Toxocara canis</i>
title_sort	<i>in vitro</i> and <i>in vivo</i> effects of <i>enterococcus faecalis</i> cect7121 on <i>toxocara canis</i>
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/37459 http://www.scielo.br/pdf/mioc/v105n5/03.pdf
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<i>In vitro</i> and <i>in vivo</i> effects of <i>Enterococcus faecalis</i> CECT7121 on <i>Toxocara canis</i>

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