Influence of the nano-micro structure of the surface on bacterial adhesion

Biomaterials failures are frequently associated to the formation of bacterial biofilms on the surface. The aim of this work is to study the adhesion of non motile bacteria streptococci consortium and motile Pseudomonas fluorescens. Substrates with micro and nanopatterned topography were used. The in...

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Autores principales: Díaz, Carolina, Cortizo, María Cecilia, Schilardi, Patricia Laura, Gómez de Saravia, Sandra Gabriela, Fernández Lorenzo de Mele, Mónica Alicia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/37223
http://www.scielo.br/pdf/mr/v10n1/03.pdf
Aporte de:
id I19-R120-10915-37223
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bioquímica
bacteria
bacterial adhesion
biofouling
extracellular polymeric material
micro/nanotopography
microtopography
nanotopography
biomaterials
spellingShingle Ciencias Exactas
Bioquímica
bacteria
bacterial adhesion
biofouling
extracellular polymeric material
micro/nanotopography
microtopography
nanotopography
biomaterials
Díaz, Carolina
Cortizo, María Cecilia
Schilardi, Patricia Laura
Gómez de Saravia, Sandra Gabriela
Fernández Lorenzo de Mele, Mónica Alicia
Influence of the nano-micro structure of the surface on bacterial adhesion
topic_facet Ciencias Exactas
Bioquímica
bacteria
bacterial adhesion
biofouling
extracellular polymeric material
micro/nanotopography
microtopography
nanotopography
biomaterials
description Biomaterials failures are frequently associated to the formation of bacterial biofilms on the surface. The aim of this work is to study the adhesion of non motile bacteria streptococci consortium and motile Pseudomonas fluorescens. Substrates with micro and nanopatterned topography were used. The influence of surface characteristics on bacterial adhesion was investigated using optical and epifluorescence microscopy, scanning electron microscopy (SEM) and atomic force microscopy (AFM). Results showed an important influence of the substratum nature. On microrough surfaces, initial bacterial adhesion was less significant than on smooth surfaces. In contrast, nanopatterned samples showed more bacterial attachment than the smooth control. It was also noted a remarkable difference in morphology, orientation and distribution of bacteria between the smooth and the nanostructured substrate. The results show the important effect of substratum nature and topography on bacterial adhesion which depended on the relation between roughness characteristics dimensions and bacterial size.
format Articulo
Articulo
author Díaz, Carolina
Cortizo, María Cecilia
Schilardi, Patricia Laura
Gómez de Saravia, Sandra Gabriela
Fernández Lorenzo de Mele, Mónica Alicia
author_facet Díaz, Carolina
Cortizo, María Cecilia
Schilardi, Patricia Laura
Gómez de Saravia, Sandra Gabriela
Fernández Lorenzo de Mele, Mónica Alicia
author_sort Díaz, Carolina
title Influence of the nano-micro structure of the surface on bacterial adhesion
title_short Influence of the nano-micro structure of the surface on bacterial adhesion
title_full Influence of the nano-micro structure of the surface on bacterial adhesion
title_fullStr Influence of the nano-micro structure of the surface on bacterial adhesion
title_full_unstemmed Influence of the nano-micro structure of the surface on bacterial adhesion
title_sort influence of the nano-micro structure of the surface on bacterial adhesion
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/37223
http://www.scielo.br/pdf/mr/v10n1/03.pdf
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