Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis

Bovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. In this study the ev...

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Autores principales: González, Ester Teresa, Licursi, María, Bonzo, Estela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Ciencias Veterinarias
bovine leukemia
Bos
Bovinae
ELISA evaluation
bovine leukemia virus
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/36725
http://www.scielo.br/pdf/bjm/v38n1/arq01.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-36725
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
bovine leukemia
Bos
Bovinae
ELISA evaluation
bovine leukemia virus
spellingShingle Ciencias Veterinarias
bovine leukemia
Bos
Bovinae
ELISA evaluation
bovine leukemia virus
González, Ester Teresa
Licursi, María
Bonzo, Estela
Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
topic_facet Ciencias Veterinarias
bovine leukemia
Bos
Bovinae
ELISA evaluation
bovine leukemia virus
description Bovine Leukosis Virus (EBLV) is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. In this study the evaluation of an indirect ELISA (Leucokit-La Plata) to detect antibodies against EBLV is presented. Comparing it with the Immunodiffusion as gold standard test, the sensitivity is 98.93%, the specificity 79.74%, the negative predictive value 99.56% and the positive predictive value 61.26%. The correspondence between both tests is 83.9% which is similar to the result mentioned by other authors (82.2%). The concordance was evaluated by calculating Kappa and Youden's J coefficients, obtaining values classified as good for both coefficients. Comparing Leucokit-La Plata and another commercial reference kit (Chekit Leucotest Bommeli AG, Bern Switzerland), the sensitivity (97.05%), specificity (94.11%), negative predictive value (92.30%) and positive predictive value (97.77%), were obtained. Applying Kappa and Jouden's Index (J) coefficients an almost perfect concordance was obtained between both kits. The Leucokit-La Plata is appropriate to apply to the commercialization of live bovines to export, bovine selection for hemo-vaccines and the implementation of control and eradication programmes.
format Articulo
Articulo
author González, Ester Teresa
Licursi, María
Bonzo, Estela
author_facet González, Ester Teresa
Licursi, María
Bonzo, Estela
author_sort González, Ester Teresa
title Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title_short Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title_full Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title_fullStr Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title_full_unstemmed Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis
title_sort enzootic bovine leukosis: performance of an indirect elisa applied in serological diagnosis
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/36725
http://www.scielo.br/pdf/bjm/v38n1/arq01.pdf
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AT licursimaria enzooticbovineleukosisperformanceofanindirectelisaappliedinserologicaldiagnosis
AT bonzoestela enzooticbovineleukosisperformanceofanindirectelisaappliedinserologicaldiagnosis
bdutipo_str Repositorios
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