Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys

Undernutrition elicited by a low-protein diet determines a marked reduction of hypophyseal activity and affects the function of the respective target organs. The objective of the present investigation was to study the ultrastructural and quantitative immunohistochemical changes of the different pitu...

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Autores principales:	Cónsole-Avegliano, Gloria Miriam, Jurado, Susana Beatriz, Oyhenart, Evelia Edith, Ferese, C., Pucciarelli, Héctor Mario, Gómez Dumm, César Leandro Alberto
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2001
Materias:	Ciencias Naturales Biología Zoología Ciencias Veterinarias pituitary gland Cebidae quantitative immunohistochemistry hypophysis squirrel monkeys monkey disease pathology ultrastructure protein calorie malnutrition undernutrition
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/36642 http://www.scielo.br/pdf/bjmbr/v34n1/3731m.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-36642
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Biología Zoología Ciencias Veterinarias pituitary gland Cebidae quantitative immunohistochemistry hypophysis squirrel monkeys monkey disease pathology ultrastructure protein calorie malnutrition undernutrition
spellingShingle	Ciencias Naturales Biología Zoología Ciencias Veterinarias pituitary gland Cebidae quantitative immunohistochemistry hypophysis squirrel monkeys monkey disease pathology ultrastructure protein calorie malnutrition undernutrition Cónsole-Avegliano, Gloria Miriam Jurado, Susana Beatriz Oyhenart, Evelia Edith Ferese, C. Pucciarelli, Héctor Mario Gómez Dumm, César Leandro Alberto Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
topic_facet	Ciencias Naturales Biología Zoología Ciencias Veterinarias pituitary gland Cebidae quantitative immunohistochemistry hypophysis squirrel monkeys monkey disease pathology ultrastructure protein calorie malnutrition undernutrition
description	Undernutrition elicited by a low-protein diet determines a marked reduction of hypophyseal activity and affects the function of the respective target organs. The objective of the present investigation was to study the ultrastructural and quantitative immunohistochemical changes of the different pituitary cell populations in undernourished monkeys that had been previously shown to have significant changes in craniofacial growth. Twenty <i>Saimiri sciureus</i> boliviensis monkeys of both sexes were used. The animals were born in captivity and were separated into two groups at one year of age, i.e., control and undernourished animals. The monkeys were fed <i>ad libitum</i> a 20% (control group) and a 10% (experimental group) protein diet for two years. Pituitaries were processed for light and electron microscopy. The former was immunolubeled with anti-GH, -PRL, -LH, -FSH, -ACTH, and -TSH sera. Volume density and cell density were measured using an image analyzer. Quantitative immunohistochemistry revealed a decrease in these parameters with regard to somatotrophs, lactotrophs, gonadotrophs and thyrotrophs from undernourished animals compared to control ones. In these populations, the ultrastructural study showed changes suggesting compensatory hyperfunction. On the contrary, no significant changes were found in the morphometric parameters or the ultrastructure of the corticotroph population. We conclude that in undernourished monkeys the somatotroph, lactotroph, gonadotroph, and thyrotroph cell populations showed quantitative immunohistochemical changes that can be correlated with ultrastructural findings.
format	Articulo Articulo
author	Cónsole-Avegliano, Gloria Miriam Jurado, Susana Beatriz Oyhenart, Evelia Edith Ferese, C. Pucciarelli, Héctor Mario Gómez Dumm, César Leandro Alberto
author_facet	Cónsole-Avegliano, Gloria Miriam Jurado, Susana Beatriz Oyhenart, Evelia Edith Ferese, C. Pucciarelli, Héctor Mario Gómez Dumm, César Leandro Alberto
author_sort	Cónsole-Avegliano, Gloria Miriam
title	Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title_short	Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title_full	Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title_fullStr	Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title_full_unstemmed	Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title_sort	morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
publishDate	2001
url	http://sedici.unlp.edu.ar/handle/10915/36642 http://www.scielo.br/pdf/bjmbr/v34n1/3731m.pdf
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Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys

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