Direct Interaction between EgFABP1, a Fatty Acid Binding Protein from <i>Echinococcus granulosus</i>, and Phospholipid Membranes

Background: Growth and maintenance of hydatid cysts produced by <i>Echinococcus granulosus</i> have a high requirement for host lipids for biosynthetic processes, membrane building and possibly cellular and developmental signalling. This requires a high degree of lipid trafficking facili...

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Autores principales: Pórfido, Jorge L., Alvite, Gabriela, Silva, Valeria, Kennedy, M., Esteves, Adriana, Córsico, Betina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Médicas
cytochrome c
Echinococcus granulosus
ionic strength
lamellar body
partition coefficient
phospholipid membrane
protein analysis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/35947
http://www.plosntds.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pntd.0001893&representation=PDF
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-35947
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
cytochrome c
Echinococcus granulosus
ionic strength
lamellar body
partition coefficient
phospholipid membrane
protein analysis
spellingShingle Ciencias Médicas
cytochrome c
Echinococcus granulosus
ionic strength
lamellar body
partition coefficient
phospholipid membrane
protein analysis
Pórfido, Jorge L.
Alvite, Gabriela
Silva, Valeria
Kennedy, M.
Esteves, Adriana
Córsico, Betina
Direct Interaction between EgFABP1, a Fatty Acid Binding Protein from <i>Echinococcus granulosus</i>, and Phospholipid Membranes
topic_facet Ciencias Médicas
cytochrome c
Echinococcus granulosus
ionic strength
lamellar body
partition coefficient
phospholipid membrane
protein analysis
description Background: Growth and maintenance of hydatid cysts produced by <i>Echinococcus granulosus</i> have a high requirement for host lipids for biosynthetic processes, membrane building and possibly cellular and developmental signalling. This requires a high degree of lipid trafficking facilitated by lipid transporter proteins. Members of the fatty acid binding protein (FABP) family have been identified in <i>Echinococcus granulosus</i>, one of which, EgFABP1 is expressed at the tegumental level in the protoscoleces, but it has also been described in both hydatid cyst fluid and secretions of protoscoleces. In spite of a considerable amount of structural and biophysical information on the FABPs in general, their specific functions remain mysterious. Methodology/Principal Findings: We have investigated the way in which EgFABP1 may interact with membranes using a variety of fluorescence-based techniques and artificial small unilamellar vesicles. We first found that bacterial recombinant EgFABP1 is loaded with fatty acids from the synthesising bacteria, and that fatty acid binding increases its resistance to proteinases, possibly due to subtle conformational changes induced on EgFABP1. By manipulating the composition of lipid vesicles and the ionic environment, we found that EgFABP1 interacts with membranes in a direct contact, collisional, manner to exchange ligand, involving both ionic and hydrophobic interactions. Moreover, we observed that the protein can compete with cytochrome c for association with the surface of small unilamellar vesicles (SUVs). Conclusions/Significance: This work constitutes a first approach to the understanding of protein-membrane interactions of EgFABP1. The results suggest that this protein may be actively involved in the exchange and transport of fatty acids between different membranes and cellular compartments within the parasite.
format Articulo
Articulo
author Pórfido, Jorge L.
Alvite, Gabriela
Silva, Valeria
Kennedy, M.
Esteves, Adriana
Córsico, Betina
author_facet Pórfido, Jorge L.
Alvite, Gabriela
Silva, Valeria
Kennedy, M.
Esteves, Adriana
Córsico, Betina
author_sort Pórfido, Jorge L.
title Direct Interaction between EgFABP1, a Fatty Acid Binding Protein from <i>Echinococcus granulosus</i>, and Phospholipid Membranes
title_short Direct Interaction between EgFABP1, a Fatty Acid Binding Protein from <i>Echinococcus granulosus</i>, and Phospholipid Membranes
title_full Direct Interaction between EgFABP1, a Fatty Acid Binding Protein from <i>Echinococcus granulosus</i>, and Phospholipid Membranes
title_fullStr Direct Interaction between EgFABP1, a Fatty Acid Binding Protein from <i>Echinococcus granulosus</i>, and Phospholipid Membranes
title_full_unstemmed Direct Interaction between EgFABP1, a Fatty Acid Binding Protein from <i>Echinococcus granulosus</i>, and Phospholipid Membranes
title_sort direct interaction between egfabp1, a fatty acid binding protein from <i>echinococcus granulosus</i>, and phospholipid membranes
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/35947
http://www.plosntds.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pntd.0001893&representation=PDF
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