A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction

Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). In Argentina, where a program to eradicate EBL has been introduced, sensitive and reliable diagnosis has attained high priority. Although the importance of the agar gel immunodiffusion test remains unchanged for r...

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Autores principales: González, Ester Teresa, Norimine, Junzo, Valera, Alejandro Rafael, Travería, Gabriel Eduardo, Oliva, Graciela A., Etcheverrigaray, María Elisa
Formato: Articulo
Lenguaje:Inglés
Publicado: 1999
Materias:
Ciencias Veterinarias
bovine leukaemia virus
diagnosis
nested-PCR
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/35945
http://www.scielo.br/pdf/pvb/v19n2/0887.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-35945
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
bovine leukaemia virus
diagnosis
nested-PCR
spellingShingle Ciencias Veterinarias
bovine leukaemia virus
diagnosis
nested-PCR
González, Ester Teresa
Norimine, Junzo
Valera, Alejandro Rafael
Travería, Gabriel Eduardo
Oliva, Graciela A.
Etcheverrigaray, María Elisa
A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction
topic_facet Ciencias Veterinarias
bovine leukaemia virus
diagnosis
nested-PCR
description Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). In Argentina, where a program to eradicate EBL has been introduced, sensitive and reliable diagnosis has attained high priority. Although the importance of the agar gel immunodiffusion test remains unchanged for routine work, an additional diagnostic technique is necessary to confirm cases of sera with equivocal results or of calves carrying maternal antibodies. Utilizing a nested shuttle polymerase chain reaction, the proviral DNA was detected from cows experimentally infected with as little as 5 ml of whole blood from BLV seropositive cows that were nonetheless normal in haematological terms. It proved to be a very sensitive technique, since it rapidly revealed the presence of the provirus, frequently at 2 weeks postinoculation and using a two-round procedure of nested PCR taking only 3 hours. Additionally, the primers used flanked a portion of the viral genome often employed to differentiate BLV type applying BamHI digestion. It is concluded that this method might offer a highly promising diagnostic tool for BLV infection.
format Articulo
Articulo
author González, Ester Teresa
Norimine, Junzo
Valera, Alejandro Rafael
Travería, Gabriel Eduardo
Oliva, Graciela A.
Etcheverrigaray, María Elisa
author_facet González, Ester Teresa
Norimine, Junzo
Valera, Alejandro Rafael
Travería, Gabriel Eduardo
Oliva, Graciela A.
Etcheverrigaray, María Elisa
author_sort González, Ester Teresa
title A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction
title_short A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction
title_full A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction
title_fullStr A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction
title_full_unstemmed A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction
title_sort rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction
publishDate 1999
url http://sedici.unlp.edu.ar/handle/10915/35945
http://www.scielo.br/pdf/pvb/v19n2/0887.pdf
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