Insulin-like growth factor-I gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas

Background: The implementation of gene therapy for the treatment of pituitary tumors emerges as a promising complement to surgery and may have distinct advantages over radiotherapy for this type of tumors. Up to now, suicide gene therapy has been the main experimental approach explored to treat expe...

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Autores principales:	Cónsole-Avegliano, Gloria Miriam, Hereñú, Claudia Beatriz, Camihort, Gisela, Luna, Georgina Cecilia, Bracamonte, María, Morel, Gustavo Ramón, Goya, Rodolfo Gustavo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2008
Materias:	Ciencias Médicas adenovirus vector green fluorescent protein peptide prolactin somatomedin C thymidine kinase hyperprolactinemia hypophysis cell immunohistochemistry prolactinoma lactotrophs
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/35097 http://www.molecular-cancer.com/content/pdf/1476-4598-7-13.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-35097
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas adenovirus vector green fluorescent protein peptide prolactin somatomedin C thymidine kinase hyperprolactinemia hypophysis cell immunohistochemistry prolactinoma lactotrophs
spellingShingle	Ciencias Médicas adenovirus vector green fluorescent protein peptide prolactin somatomedin C thymidine kinase hyperprolactinemia hypophysis cell immunohistochemistry prolactinoma lactotrophs Cónsole-Avegliano, Gloria Miriam Hereñú, Claudia Beatriz Camihort, Gisela Luna, Georgina Cecilia Bracamonte, María Morel, Gustavo Ramón Goya, Rodolfo Gustavo Insulin-like growth factor-I gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas
topic_facet	Ciencias Médicas adenovirus vector green fluorescent protein peptide prolactin somatomedin C thymidine kinase hyperprolactinemia hypophysis cell immunohistochemistry prolactinoma lactotrophs
description	Background: The implementation of gene therapy for the treatment of pituitary tumors emerges as a promising complement to surgery and may have distinct advantages over radiotherapy for this type of tumors. Up to now, suicide gene therapy has been the main experimental approach explored to treat experimental pituitary tumors. In the present study we assessed the effectiveness of insulin-like growth factor I (IGF-I) gene therapy for the treatment of estrogen-induced prolactinomas in rats. Results: Female Sprague Dawley rats were subcutaneously implanted with silastic capsules filled with 17-β estradiol (E2) in order to induce pituitary prolactinomas. Blood samples were taken at regular intervals in order to measure serum prolactin (PRL). As expected, serum PRL increased progressively and 23 days after implanting the E2 capsules (Experimental day 0), circulating PRL had undergone a 3-4 fold increase. On Experimental day 0 part of the E2-implanted animals received a bilateral intrapituitary injection of either an adenoviral vector expressing the gene for rat IGF-I (RAd-IGFI), or a vector (RAd-GFP) expressing the gene for green fluorescent protein (GFP). Seven days post vector injection all animals were sacrificed and their pituitaries morphometrically analyzed to evaluate changes in the lactotroph population. RAd-IGFI but not RAd-GFP, induced a significant fall in serum PRL. Furthermore, RAd-IGFI but not RAd-GFP significantly reversed the increase in lactotroph size (CS) and volume density (VD) induced by E2 treatment. Conclusion: We conclude that IGF-I gene therapy constitutes a potentially useful intervention for the treatment of prolactinomas and that bioactive peptide gene delivery may open novel therapeutic avenues for the treatment of pituitary tumors.
format	Articulo Articulo
author	Cónsole-Avegliano, Gloria Miriam Hereñú, Claudia Beatriz Camihort, Gisela Luna, Georgina Cecilia Bracamonte, María Morel, Gustavo Ramón Goya, Rodolfo Gustavo
author_facet	Cónsole-Avegliano, Gloria Miriam Hereñú, Claudia Beatriz Camihort, Gisela Luna, Georgina Cecilia Bracamonte, María Morel, Gustavo Ramón Goya, Rodolfo Gustavo
author_sort	Cónsole-Avegliano, Gloria Miriam
title	Insulin-like growth factor-I gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas
title_short	Insulin-like growth factor-I gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas
title_full	Insulin-like growth factor-I gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas
title_fullStr	Insulin-like growth factor-I gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas
title_full_unstemmed	Insulin-like growth factor-I gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas
title_sort	insulin-like growth factor-i gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas
publishDate	2008
url	http://sedici.unlp.edu.ar/handle/10915/35097 http://www.molecular-cancer.com/content/pdf/1476-4598-7-13.pdf
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Insulin-like growth factor-I gene therapy reverses morphologic changes and reduces hyperprolactinemia in experimental rat prolactinomas

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