A simple method to assess the oxidative susceptibility of low density lipoproteins

Background: Oxidative modification of low density lipoproteins (LDL) is recognized as one of the major processes involved in atherogenesis. The in vitro standardized measurement of LDL oxidative susceptibility could thus be of clinical significance. The aim of the present study was to establish a me...

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Autores principales:	Scoccia, Adriana E., Molinuevo, María Silvina, McCarthy, Antonio Desmond, Cortizo, Ana María
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2001
Materias:	Bioquímica Ciencias Exactas hydrogen peroxide low density lipoprotein thiobarbituric acid reactive substance chemical reaction kinetics genetic susceptibility oxidative stress
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/34607 http://www.biomedcentral.com/1472-6890/1/1
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-34607
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Bioquímica Ciencias Exactas hydrogen peroxide low density lipoprotein thiobarbituric acid reactive substance chemical reaction kinetics genetic susceptibility oxidative stress
spellingShingle	Bioquímica Ciencias Exactas hydrogen peroxide low density lipoprotein thiobarbituric acid reactive substance chemical reaction kinetics genetic susceptibility oxidative stress Scoccia, Adriana E. Molinuevo, María Silvina McCarthy, Antonio Desmond Cortizo, Ana María A simple method to assess the oxidative susceptibility of low density lipoproteins
topic_facet	Bioquímica Ciencias Exactas hydrogen peroxide low density lipoprotein thiobarbituric acid reactive substance chemical reaction kinetics genetic susceptibility oxidative stress
description	Background: Oxidative modification of low density lipoproteins (LDL) is recognized as one of the major processes involved in atherogenesis. The in vitro standardized measurement of LDL oxidative susceptibility could thus be of clinical significance. The aim of the present study was to establish a method which would allow the evaluation of oxidative susceptibility of LDL in the general clinical laboratory. Results: LDL was isolated from human plasma by selective precipitation with amphipathic polymers. The ability of LDL to form peroxides was assessed by measuring thiobarbituric acid reactive substances (TBARS) after incubation with Cu2+ and H2O2. Reaction kinetics showed a three-phase pattern (latency, propagation and decomposition phases) which allowed us to select 150 min as the time point to stop the incubation by cooling and EDTA addition. The mixture Cu 2+/H2O2 yielded more lipoperoxides than each one on its own at the same time end-point. Induced peroxidation was measured in normal subjects and in type 2 diabetic patients. In the control group, results were 21.7 ± 1.5 nmol MDA/mg LDL protein, while in the diabetic group results were significantly increased (39.0 ± 3.0 nmol MDA/mg LDL protein; p < 0.001). Conclusion: a simple and useful method is presented for the routine determination of LDL susceptibility to peroxidation in a clinical laboratory.
format	Articulo Articulo
author	Scoccia, Adriana E. Molinuevo, María Silvina McCarthy, Antonio Desmond Cortizo, Ana María
author_facet	Scoccia, Adriana E. Molinuevo, María Silvina McCarthy, Antonio Desmond Cortizo, Ana María
author_sort	Scoccia, Adriana E.
title	A simple method to assess the oxidative susceptibility of low density lipoproteins
title_short	A simple method to assess the oxidative susceptibility of low density lipoproteins
title_full	A simple method to assess the oxidative susceptibility of low density lipoproteins
title_fullStr	A simple method to assess the oxidative susceptibility of low density lipoproteins
title_full_unstemmed	A simple method to assess the oxidative susceptibility of low density lipoproteins
title_sort	simple method to assess the oxidative susceptibility of low density lipoproteins
publishDate	2001
url	http://sedici.unlp.edu.ar/handle/10915/34607 http://www.biomedcentral.com/1472-6890/1/1
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A simple method to assess the oxidative susceptibility of low density lipoproteins

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