Cooperative binding of transcription factors promotes bimodal gene expression response
In the present work we extend and analyze the scope of our recently proposed stochastic model for transcriptional regulation, which considers an arbitrarily complex cis-regulatory system using only elementary reactions. Previously, we determined the role of cooperativity on the intrinsic fluctuation...
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| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2012
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/29762 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0044812 |
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I19-R120-10915-29762 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Física Factores de Transcripción Proteínas de Unión al ADN ARN Mensajero |
| spellingShingle |
Ciencias Exactas Física Factores de Transcripción Proteínas de Unión al ADN ARN Mensajero Gutiérrez, Pablo S. Monteoliva, Diana Diambra, Luis Aníbal Cooperative binding of transcription factors promotes bimodal gene expression response |
| topic_facet |
Ciencias Exactas Física Factores de Transcripción Proteínas de Unión al ADN ARN Mensajero |
| description |
In the present work we extend and analyze the scope of our recently proposed stochastic model for transcriptional regulation, which considers an arbitrarily complex cis-regulatory system using only elementary reactions. Previously, we determined the role of cooperativity on the intrinsic fluctuations of gene expression for activating transcriptional switches, by means of master equation formalism and computer simulation. This model allowed us to distinguish between two cooperative binding mechanisms and, even though the mean expression levels were not affected differently by the acting mechanism, we showed that the associated fluctuations were different. In the present generalized model we include other regulatory functions in addition to those associated to an activator switch. Namely, we introduce repressive regulatory functions and two theoretical mechanisms that account for the biphasic response that some cis-regulatory systems show to the transcription factor concentration. We have also extended our previous master equation formalism in order to include protein production by stochastic translation of mRNA. Furthermore, we examine the graded/binary scenarios in the context of the interaction energy between transcription factors. In this sense, this is the first report to show that the cooperative binding of transcription factors to DNA promotes the "all-or-none" phenomenon observed in eukaryotic systems. In addition, we confirm that gene expression fluctuation levels associated with one of two cooperative binding mechanism never exceed the fluctuation levels of the other. |
| format |
Articulo Articulo |
| author |
Gutiérrez, Pablo S. Monteoliva, Diana Diambra, Luis Aníbal |
| author_facet |
Gutiérrez, Pablo S. Monteoliva, Diana Diambra, Luis Aníbal |
| author_sort |
Gutiérrez, Pablo S. |
| title |
Cooperative binding of transcription factors promotes bimodal gene expression response |
| title_short |
Cooperative binding of transcription factors promotes bimodal gene expression response |
| title_full |
Cooperative binding of transcription factors promotes bimodal gene expression response |
| title_fullStr |
Cooperative binding of transcription factors promotes bimodal gene expression response |
| title_full_unstemmed |
Cooperative binding of transcription factors promotes bimodal gene expression response |
| title_sort |
cooperative binding of transcription factors promotes bimodal gene expression response |
| publishDate |
2012 |
| url |
http://sedici.unlp.edu.ar/handle/10915/29762 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0044812 |
| work_keys_str_mv |
AT gutierrezpablos cooperativebindingoftranscriptionfactorspromotesbimodalgeneexpressionresponse AT monteolivadiana cooperativebindingoftranscriptionfactorspromotesbimodalgeneexpressionresponse AT diambraluisanibal cooperativebindingoftranscriptionfactorspromotesbimodalgeneexpressionresponse |
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Repositorios |
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1764820468199587842 |