Glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols

Background: De novo glycerolipid synthesis begins with the acylation of glycerol-3 phosphate catalyzed by glycerol-3-phosphate acyltransferase (GPAT). In mammals, at least four GPAT isoforms have been described, differing in their cell and tissue locations and sensitivity to sulfhydryl reagents. In...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Cattaneo, Elizabeth Renee, Pellón Maisón, Magalí, Rabassa, Martín Enrique, Lacunza, Ezequiel, Coleman, Rosalind A., González Baró, María del Rosario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Médicas
Bioquímica
Aciltransferasas
Ácidos Grasos
ARN Mensajero
Mitocondrias
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/29608
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0042986
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-29608
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Bioquímica
Aciltransferasas
Ácidos Grasos
ARN Mensajero
Mitocondrias
spellingShingle Ciencias Médicas
Bioquímica
Aciltransferasas
Ácidos Grasos
ARN Mensajero
Mitocondrias
Cattaneo, Elizabeth Renee
Pellón Maisón, Magalí
Rabassa, Martín Enrique
Lacunza, Ezequiel
Coleman, Rosalind A.
González Baró, María del Rosario
Glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols
topic_facet Ciencias Médicas
Bioquímica
Aciltransferasas
Ácidos Grasos
ARN Mensajero
Mitocondrias
description Background: De novo glycerolipid synthesis begins with the acylation of glycerol-3 phosphate catalyzed by glycerol-3-phosphate acyltransferase (GPAT). In mammals, at least four GPAT isoforms have been described, differing in their cell and tissue locations and sensitivity to sulfhydryl reagents. In this work we show that mitochondrial GPAT2 overexpression in CHO-K1 cells increased TAG content and both GPAT and AGPAT activities 2-fold with arachidonoyl-CoA as a substrate, indicating specificity for this fatty acid. Methods and Results: Incubation of GPAT2-transfected CHO-K1 cells with [1-14C]arachidonate for 3 h increased incorporation of [14C]arachidonate into TAG by 40%. Consistently, arachidonic acid was present in the TAG fraction of cells that overexpressed GPAT2, but not in control cells, corroborating GPAT2's role in synthesizing TAG that is rich in arachidonic acid. In rat and mouse testis, Gpat2 mRNA was expressed only in primary spermatocytes; the protein was also detected in late stages of spermatogenesis. During rat sexual maturation, both the testicular TAG content and the arachidonic acid content in the TAG fraction peaked at 30 d, matching the highest expression of Gpat2 mRNA and protein. Conclusions: These results strongly suggest that GPAT2 expression is linked to arachidonoyl-CoA incorporation into TAG in spermatogenic germ cells.
format Articulo
Articulo
author Cattaneo, Elizabeth Renee
Pellón Maisón, Magalí
Rabassa, Martín Enrique
Lacunza, Ezequiel
Coleman, Rosalind A.
González Baró, María del Rosario
author_facet Cattaneo, Elizabeth Renee
Pellón Maisón, Magalí
Rabassa, Martín Enrique
Lacunza, Ezequiel
Coleman, Rosalind A.
González Baró, María del Rosario
author_sort Cattaneo, Elizabeth Renee
title Glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols
title_short Glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols
title_full Glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols
title_fullStr Glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols
title_full_unstemmed Glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols
title_sort glycerol-3-phosphate acyltransferase-2 is expressed in spermatic germ cells and incorporates arachidonic acid into triacylglycerols
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/29608
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0042986
work_keys_str_mv AT cattaneoelizabethrenee glycerol3phosphateacyltransferase2isexpressedinspermaticgermcellsandincorporatesarachidonicacidintotriacylglycerols
AT pellonmaisonmagali glycerol3phosphateacyltransferase2isexpressedinspermaticgermcellsandincorporatesarachidonicacidintotriacylglycerols
AT rabassamartinenrique glycerol3phosphateacyltransferase2isexpressedinspermaticgermcellsandincorporatesarachidonicacidintotriacylglycerols
AT lacunzaezequiel glycerol3phosphateacyltransferase2isexpressedinspermaticgermcellsandincorporatesarachidonicacidintotriacylglycerols
AT colemanrosalinda glycerol3phosphateacyltransferase2isexpressedinspermaticgermcellsandincorporatesarachidonicacidintotriacylglycerols
AT gonzalezbaromariadelrosario glycerol3phosphateacyltransferase2isexpressedinspermaticgermcellsandincorporatesarachidonicacidintotriacylglycerols
bdutipo_str Repositorios
_version_ 1764820468320174085

Ejemplares similares