Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach

α-Hemolysin (HlyA) from Escherichia coli is considered as the prototype of a family of toxins called RTX (repeat in toxin), a group of proteins that share genetic and structural features. HlyA is an important virulence factor in E. coli extraintestinal infections, such as meningitis, septicemia and...

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Detalles Bibliográficos
Autores principales: Sánchez, Susana, Bakás, Laura Susana, Gratton, Enrico, Herlax, Vanesa Silvana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Ciencias Médicas
Bioquímica
Calcio
Citotoxinas
Microscopía Fluorescente
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/29560
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021127
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-29560
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Bioquímica
Calcio
Citotoxinas
Microscopía Fluorescente
spellingShingle Ciencias Médicas
Bioquímica
Calcio
Citotoxinas
Microscopía Fluorescente
Sánchez, Susana
Bakás, Laura Susana
Gratton, Enrico
Herlax, Vanesa Silvana
Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach
topic_facet Ciencias Médicas
Bioquímica
Calcio
Citotoxinas
Microscopía Fluorescente
description α-Hemolysin (HlyA) from Escherichia coli is considered as the prototype of a family of toxins called RTX (repeat in toxin), a group of proteins that share genetic and structural features. HlyA is an important virulence factor in E. coli extraintestinal infections, such as meningitis, septicemia and urinary infections. High concentrations of the toxin cause the lysis of several cells such as erythrocytes, granulocytes, monocytes, endothelial and renal epithelial cells of different species. At low concentrations it induces the production of cytokines and apoptosis. Since many of the subcytolytic effects in other cells have been reported to be triggered by the increase of intracellular calcium, we followed the calcium concentration inside the erythrocytes while incubating with sublytic concentrations of HlyA. Calcium concentration was monitored using the calcium indicator Green 1, 2-photon excitation, and fluorescence lifetime imaging microscopy (FLIM). Data were analyzed using the phasor representation. In this report, we present evidence that, at sublytic concentrations, HlyA induces an increase of calcium concentration in rabbit erythrocytes in the first 10 s. Results are discussed in relation to the difficulties of measuring calcium concentrations in erythrocytes where hemoglobin is present, the contribution of the background and the heterogeneity of the response observed in individual cells.
format Articulo
Articulo
author Sánchez, Susana
Bakás, Laura Susana
Gratton, Enrico
Herlax, Vanesa Silvana
author_facet Sánchez, Susana
Bakás, Laura Susana
Gratton, Enrico
Herlax, Vanesa Silvana
author_sort Sánchez, Susana
title Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach
title_short Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach
title_full Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach
title_fullStr Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach
title_full_unstemmed Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach
title_sort alpha hemolysin induces an increase of erythrocytes calcium: a flim 2-photon phasor analysis approach
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/29560
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021127
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AT bakaslaurasusana alphahemolysininducesanincreaseoferythrocytescalciumaflim2photonphasoranalysisapproach
AT grattonenrico alphahemolysininducesanincreaseoferythrocytescalciumaflim2photonphasoranalysisapproach
AT herlaxvanesasilvana alphahemolysininducesanincreaseoferythrocytescalciumaflim2photonphasoranalysisapproach
bdutipo_str Repositorios
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