Evaluation and additional recommendations for preparing a whole blood control material

Objective: The assessment of an easy to prepare and low cost control material for Hematology, available for manual and automated methods. Material and Method: Aliquots of stabilized whole blood were prepared by partial fixation with aldehydes; the stability at different temperatures (4. 20 and 37 °...

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Autores principales: Fink, Nilda Esther, Fernández Alberti, María Alejandra, Crispini, Isabel, Cabutti, Norberto V., Mazziotta, Daniel
Formato: Articulo
Lenguaje:Inglés
Publicado: 1998
Materias:
Ciencias Exactas
Equipo de Laboratorio
Sangre
Control de Calidad
Hematología
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/29367
http://www.scielo.br/pdf/rsp/v32n2/p107-111.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-29367
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Equipo de Laboratorio
Sangre
Control de Calidad
Hematología
spellingShingle Ciencias Exactas
Equipo de Laboratorio
Sangre
Control de Calidad
Hematología
Fink, Nilda Esther
Fernández Alberti, María Alejandra
Crispini, Isabel
Cabutti, Norberto V.
Mazziotta, Daniel
Evaluation and additional recommendations for preparing a whole blood control material
topic_facet Ciencias Exactas
Equipo de Laboratorio
Sangre
Control de Calidad
Hematología
description Objective: The assessment of an easy to prepare and low cost control material for Hematology, available for manual and automated methods. Material and Method: Aliquots of stabilized whole blood were prepared by partial fixation with aldehydes; the stability at different temperatures (4. 20 and 37 °C) during periods of up to 8-9 weeks and aliquot variability with both methods were controlled. Results: Aliquot variability with automated methods at day 1, expressed as CV% (coefficient of variation) was: white blood cells (WBC) 2.7, red blood cells (RBC) 0.7, hemoglobin (Hb) 0.6, hematocrit (Hct) 0.7, mean cell volume (MCV) 0.3, mean cell hemoglobin (MCH) 0.6, mean cell hemoglobin concentration (MCHC) 0.7, and platelets (PLT) 4.6. The CV (coefficient of variation) percentages obtained with manual methods in one of the batches were: WBC 23, Hct 2.8, Hb 4.5, MCHC 5.9, PLT 41. Samples stored at 4ºC and 20ºC showed good stability, only a very low initial hemolysis being observed, whereas those stored at 37ºC deteriobed a rapidly (metahemoglobin formation, aggregation of WBC and platelets, as well as alteration of erythrocyte indexes). Conclusions: It was confirmed that, as long as there is no exposure to high temperatures during distribution, this material is stable, allowing assessment, both esternal and internal, for control purposes, with acceptable reproductivity, both for manual and auttomatic methods.
format Articulo
Articulo
author Fink, Nilda Esther
Fernández Alberti, María Alejandra
Crispini, Isabel
Cabutti, Norberto V.
Mazziotta, Daniel
author_facet Fink, Nilda Esther
Fernández Alberti, María Alejandra
Crispini, Isabel
Cabutti, Norberto V.
Mazziotta, Daniel
author_sort Fink, Nilda Esther
title Evaluation and additional recommendations for preparing a whole blood control material
title_short Evaluation and additional recommendations for preparing a whole blood control material
title_full Evaluation and additional recommendations for preparing a whole blood control material
title_fullStr Evaluation and additional recommendations for preparing a whole blood control material
title_full_unstemmed Evaluation and additional recommendations for preparing a whole blood control material
title_sort evaluation and additional recommendations for preparing a whole blood control material
publishDate 1998
url http://sedici.unlp.edu.ar/handle/10915/29367
http://www.scielo.br/pdf/rsp/v32n2/p107-111.pdf
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AT mazziottadaniel evaluationandadditionalrecommendationsforpreparingawholebloodcontrolmaterial
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