Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis

The aim of this study was to evaluate a wide panel of antigens of Mycobacterium avium subsp. paratuberculosis (MAP) to select candidates for the diagnosis of paratuberculosis (PTB). A total of 54 recombinant proteins were spotted onto nitrocellulose membranes and exposed to sera from animals with PT...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Mon, María Laura, Viale, Mariana, Baschetti, Guido, Alvarado Pinedo, Fiorella, Gioffre, Andrea, Travería, Gabriel Eduardo, Willemsen, Peter, Bakker, Douwe, Romano, María Isabel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Veterinarias
Mycobacterium avium
Bacterias
Paratuberculosis
Antígenos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/29177
http://www.hindawi.com/journals/vmi/2012/860362/
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-29177
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Mycobacterium avium
Bacterias
Paratuberculosis
Antígenos
spellingShingle Ciencias Veterinarias
Mycobacterium avium
Bacterias
Paratuberculosis
Antígenos
Mon, María Laura
Viale, Mariana
Baschetti, Guido
Alvarado Pinedo, Fiorella
Gioffre, Andrea
Travería, Gabriel Eduardo
Willemsen, Peter
Bakker, Douwe
Romano, María Isabel
Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
topic_facet Ciencias Veterinarias
Mycobacterium avium
Bacterias
Paratuberculosis
Antígenos
description The aim of this study was to evaluate a wide panel of antigens of Mycobacterium avium subsp. paratuberculosis (MAP) to select candidates for the diagnosis of paratuberculosis (PTB). A total of 54 recombinant proteins were spotted onto nitrocellulose membranes and exposed to sera from animals with PTB (n=25), healthy animals (n=10), and animals experimentally infected with M. bovis (n=8). This initial screening allowed us to select seven antigens: MAP 2513, MAP 1693, MAP 2020, MAP 0038, MAP 1272, MAP 0209c, and MAP 0210c, which reacted with sera from animals with PTB and showed little cross-reactivity with sera from healthy animals and animals experimentally infected with M. bovis. The second step was to evaluate the antigen cocktail of these seven antigens by ELISA. For this evaluation, we used sera from animals with PTB (n=25), healthy animals (n=26), and animals experimentally infected with M. bovis (n=17). Using ELISA, the cocktail of the seven selected MAP antigens reacted with sera from 18 of the 25 animals with PTB and did not exhibit cross-reactivity with healthy animals and only low reactivity with animals with bovine tuberculosis. The combined application of these antigens could form part of a test which may help in the diagnosis of PTB.
format Articulo
Articulo
author Mon, María Laura
Viale, Mariana
Baschetti, Guido
Alvarado Pinedo, Fiorella
Gioffre, Andrea
Travería, Gabriel Eduardo
Willemsen, Peter
Bakker, Douwe
Romano, María Isabel
author_facet Mon, María Laura
Viale, Mariana
Baschetti, Guido
Alvarado Pinedo, Fiorella
Gioffre, Andrea
Travería, Gabriel Eduardo
Willemsen, Peter
Bakker, Douwe
Romano, María Isabel
author_sort Mon, María Laura
title Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title_short Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title_full Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title_fullStr Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title_full_unstemmed Search for Mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
title_sort search for mycobacterium avium subspecies paratuberculosis antigens for the diagnosis of paratuberculosis
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/29177
http://www.hindawi.com/journals/vmi/2012/860362/
work_keys_str_mv AT monmarialaura searchformycobacteriumaviumsubspeciesparatuberculosisantigensforthediagnosisofparatuberculosis
AT vialemariana searchformycobacteriumaviumsubspeciesparatuberculosisantigensforthediagnosisofparatuberculosis
AT baschettiguido searchformycobacteriumaviumsubspeciesparatuberculosisantigensforthediagnosisofparatuberculosis
AT alvaradopinedofiorella searchformycobacteriumaviumsubspeciesparatuberculosisantigensforthediagnosisofparatuberculosis
AT gioffreandrea searchformycobacteriumaviumsubspeciesparatuberculosisantigensforthediagnosisofparatuberculosis
AT traveriagabrieleduardo searchformycobacteriumaviumsubspeciesparatuberculosisantigensforthediagnosisofparatuberculosis
AT willemsenpeter searchformycobacteriumaviumsubspeciesparatuberculosisantigensforthediagnosisofparatuberculosis
AT bakkerdouwe searchformycobacteriumaviumsubspeciesparatuberculosisantigensforthediagnosisofparatuberculosis
AT romanomariaisabel searchformycobacteriumaviumsubspeciesparatuberculosisantigensforthediagnosisofparatuberculosis
bdutipo_str Repositorios
_version_ 1764820467929055234

Ejemplares similares