Determination of tolbutamide and hydroxytolbutamide by LC–MS/MS in rat and its application to assessment of CYP2C9 activity

A sensitive and selective liquid chromatography–tandem mass spectrometry method (LC–MS/MS) for the determination of tolbutamide (TB) and its metabolite hydroxytolbutamide (HTB) in rat plasma was developed using carbamazepine as an internal standard. Chromatographic separation was performed by an Agi...

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Detalles Bibliográficos
Autores principales: Qiu, Xiangjun, Fei, Ai-li, Xu, Zhisheng, Du, Xiaoxiang, Xu, Ren-ai, Zhu, Haiyan
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
CYP
Hydroxytolbutamide
LC-MS/MS
Rat
Tolbutamide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/25744
http://www.latamjpharm.org/resumenes/31/7/LAJOP_31_7_1_11.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-25744
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
CYP
Hydroxytolbutamide
LC-MS/MS
Rat
Tolbutamide
spellingShingle Farmacia
CYP
Hydroxytolbutamide
LC-MS/MS
Rat
Tolbutamide
Qiu, Xiangjun
Fei, Ai-li
Xu, Zhisheng
Du, Xiaoxiang
Xu, Ren-ai
Zhu, Haiyan
Determination of tolbutamide and hydroxytolbutamide by LC–MS/MS in rat and its application to assessment of CYP2C9 activity
topic_facet Farmacia
CYP
Hydroxytolbutamide
LC-MS/MS
Rat
Tolbutamide
description A sensitive and selective liquid chromatography–tandem mass spectrometry method (LC–MS/MS) for the determination of tolbutamide (TB) and its metabolite hydroxytolbutamide (HTB) in rat plasma was developed using carbamazepine as an internal standard. Chromatographic separation was performed by an Agilent Zorbax SB-C18 column (150 mmx2.1 mm, 3.5 μm), using the gradient elution of 0.1 % formic acid in water and acetonitrile. Calibration plots were linear over range of 5–1000 ng/mL for TB and 10–2000 ng/mL for HTB in rat plasma. The intra- and inter-day relative standard deviations of the assay were less than 10 % for both TB and HTB. The validated method is successfully used to analyze the influence of bupropion on cytochrome P450-mediated metabolism of TB. The biotransformation rates of TB administered either separately or both simultaneously were compared in this study. The results revealed that bupropion had no significant effect on TB hydroxylation.
format Articulo
Articulo
author Qiu, Xiangjun
Fei, Ai-li
Xu, Zhisheng
Du, Xiaoxiang
Xu, Ren-ai
Zhu, Haiyan
author_facet Qiu, Xiangjun
Fei, Ai-li
Xu, Zhisheng
Du, Xiaoxiang
Xu, Ren-ai
Zhu, Haiyan
author_sort Qiu, Xiangjun
title Determination of tolbutamide and hydroxytolbutamide by LC–MS/MS in rat and its application to assessment of CYP2C9 activity
title_short Determination of tolbutamide and hydroxytolbutamide by LC–MS/MS in rat and its application to assessment of CYP2C9 activity
title_full Determination of tolbutamide and hydroxytolbutamide by LC–MS/MS in rat and its application to assessment of CYP2C9 activity
title_fullStr Determination of tolbutamide and hydroxytolbutamide by LC–MS/MS in rat and its application to assessment of CYP2C9 activity
title_full_unstemmed Determination of tolbutamide and hydroxytolbutamide by LC–MS/MS in rat and its application to assessment of CYP2C9 activity
title_sort determination of tolbutamide and hydroxytolbutamide by lc–ms/ms in rat and its application to assessment of cyp2c9 activity
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/25744
http://www.latamjpharm.org/resumenes/31/7/LAJOP_31_7_1_11.pdf
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AT xuzhisheng determinationoftolbutamideandhydroxytolbutamidebylcmsmsinratanditsapplicationtoassessmentofcyp2c9activity
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bdutipo_str Repositorios
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