Determination of dezocine in rabbit plasma by liquid chromatography-mass spectrometry and its application

A sensitive and selective liquid chromatography-mass spectrometry (LC–MS) method for determination of dezocine in rabbit plasma was developed and validated. After addition of diazepam as internal standard (IS), liquid–liquid extraction (LLE) was used for sample preparation, and chromatography involv...

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Autores principales: Zhang, Yuan, Zheng, Yuancai, Chen, Tongke, Zhu, Jiayin, Zhao, Huiling, Lin, Guanyang
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
LC-MS
dezocine
pharmacokinetics
rabbit plasma
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/25731
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_2_6.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-25731
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
LC-MS
dezocine
pharmacokinetics
rabbit plasma
spellingShingle Farmacia
LC-MS
dezocine
pharmacokinetics
rabbit plasma
Zhang, Yuan
Zheng, Yuancai
Chen, Tongke
Zhu, Jiayin
Zhao, Huiling
Lin, Guanyang
Determination of dezocine in rabbit plasma by liquid chromatography-mass spectrometry and its application
topic_facet Farmacia
LC-MS
dezocine
pharmacokinetics
rabbit plasma
description A sensitive and selective liquid chromatography-mass spectrometry (LC–MS) method for determination of dezocine in rabbit plasma was developed and validated. After addition of diazepam as internal standard (IS), liquid–liquid extraction (LLE) was used for sample preparation, and chromatography involved Agilent SB-C18 column (2.1 mmx50 mm, 3.5 um) using 0.1 % formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragment ions m/z 245.8 for dezocine and m/z 284.8 for diazepam (internal standard, IS). The assay was linear over the range of 5–500 ng/mL for dezocine, with a lower limit of quantitation (LLOQ) of 5 ng/mL for dezocine. Intra- and inter-day precisions were less than 13 % and the accuracies were in the range of 93.1-105.2 % for dezocine. This developed method was successfully applied for the determination of dezocine in rabbit plasma for pharmacokinetic study.
format Articulo
Comunicacion
author Zhang, Yuan
Zheng, Yuancai
Chen, Tongke
Zhu, Jiayin
Zhao, Huiling
Lin, Guanyang
author_facet Zhang, Yuan
Zheng, Yuancai
Chen, Tongke
Zhu, Jiayin
Zhao, Huiling
Lin, Guanyang
author_sort Zhang, Yuan
title Determination of dezocine in rabbit plasma by liquid chromatography-mass spectrometry and its application
title_short Determination of dezocine in rabbit plasma by liquid chromatography-mass spectrometry and its application
title_full Determination of dezocine in rabbit plasma by liquid chromatography-mass spectrometry and its application
title_fullStr Determination of dezocine in rabbit plasma by liquid chromatography-mass spectrometry and its application
title_full_unstemmed Determination of dezocine in rabbit plasma by liquid chromatography-mass spectrometry and its application
title_sort determination of dezocine in rabbit plasma by liquid chromatography-mass spectrometry and its application
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/25731
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_2_6.pdf
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