A simple HPLC method for the determination of pentyl gallate and identification of an alkaline degradant
Pentyl gallate (PG) is a gallic acid n-alkyl ester with a promising antiherpetic topical activity. No method for PG quantification is described in the literature. Therefore, the objective of this investigation is to develop and validate a simple method for determination of PG by High performance li...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2012
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/25729 http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_2_4.pdf |
| Aporte de: |
| Sumario: | Pentyl gallate (PG) is a gallic acid n-alkyl ester with a promising antiherpetic topical activity.
No method for PG quantification is described in the literature. Therefore, the objective of this investigation is to develop and validate a simple method for determination of PG by High performance liquid chromatography (HPLC) considering its future application in therapy. A nanoemulsion was proposed as PG delivery system due to its low aqueous solubility that limits its delivery through the skin. Chromatographic separation was achieved on a C18 column with 50:49:1 (%, v/v) methanol:water:trifluoroacetic acid (TFA) as mobile phase at a flow rate of 1.2 mL/min; detection at 275 nm. The method was validated for linearity, precision, accuracy, robustness and specificity. The degradant detected under alkaline condition was structurally characterized by time-of-flight mass spectrometry (TOF-MS). All results indicate the method is adequate for PG analysis. |
|---|