A simple HPLC method for the determination of pentyl gallate and identification of an alkaline degradant

Pentyl gallate (PG) is a gallic acid n-alkyl ester with a promising antiherpetic topical activity. No method for PG quantification is described in the literature. Therefore, the objective of this investigation is to develop and validate a simple method for determination of PG by High performance li...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Dias, Daiane O., Nunes, Ricardo José, Teixeira, Helder, Bassani, Valquiria L., Simões, Cláudia Maria Oliveira, Koester, Letícia S., Kelmann, Regina G.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
HPLC
Nanoemulsion
Pentyl gallate
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/25729
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_2_4.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-25729
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
HPLC
Nanoemulsion
Pentyl gallate
spellingShingle Farmacia
HPLC
Nanoemulsion
Pentyl gallate
Dias, Daiane O.
Nunes, Ricardo José
Teixeira, Helder
Bassani, Valquiria L.
Simões, Cláudia Maria Oliveira
Koester, Letícia S.
Kelmann, Regina G.
A simple HPLC method for the determination of pentyl gallate and identification of an alkaline degradant
topic_facet Farmacia
HPLC
Nanoemulsion
Pentyl gallate
description Pentyl gallate (PG) is a gallic acid n-alkyl ester with a promising antiherpetic topical activity. No method for PG quantification is described in the literature. Therefore, the objective of this investigation is to develop and validate a simple method for determination of PG by High performance liquid chromatography (HPLC) considering its future application in therapy. A nanoemulsion was proposed as PG delivery system due to its low aqueous solubility that limits its delivery through the skin. Chromatographic separation was achieved on a C18 column with 50:49:1 (%, v/v) methanol:water:trifluoroacetic acid (TFA) as mobile phase at a flow rate of 1.2 mL/min; detection at 275 nm. The method was validated for linearity, precision, accuracy, robustness and specificity. The degradant detected under alkaline condition was structurally characterized by time-of-flight mass spectrometry (TOF-MS). All results indicate the method is adequate for PG analysis.
format Articulo
Comunicacion
author Dias, Daiane O.
Nunes, Ricardo José
Teixeira, Helder
Bassani, Valquiria L.
Simões, Cláudia Maria Oliveira
Koester, Letícia S.
Kelmann, Regina G.
author_facet Dias, Daiane O.
Nunes, Ricardo José
Teixeira, Helder
Bassani, Valquiria L.
Simões, Cláudia Maria Oliveira
Koester, Letícia S.
Kelmann, Regina G.
author_sort Dias, Daiane O.
title A simple HPLC method for the determination of pentyl gallate and identification of an alkaline degradant
title_short A simple HPLC method for the determination of pentyl gallate and identification of an alkaline degradant
title_full A simple HPLC method for the determination of pentyl gallate and identification of an alkaline degradant
title_fullStr A simple HPLC method for the determination of pentyl gallate and identification of an alkaline degradant
title_full_unstemmed A simple HPLC method for the determination of pentyl gallate and identification of an alkaline degradant
title_sort simple hplc method for the determination of pentyl gallate and identification of an alkaline degradant
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/25729
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_2_4.pdf
work_keys_str_mv AT diasdaianeo asimplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT nunesricardojose asimplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT teixeirahelder asimplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT bassanivalquirial asimplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT simoesclaudiamariaoliveira asimplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT koesterleticias asimplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT kelmannreginag asimplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT diasdaianeo simplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT nunesricardojose simplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT teixeirahelder simplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT bassanivalquirial simplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT simoesclaudiamariaoliveira simplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT koesterleticias simplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
AT kelmannreginag simplehplcmethodforthedeterminationofpentylgallateandidentificationofanalkalinedegradant
bdutipo_str Repositorios
_version_ 1764820466847973376

Ejemplares similares