Pharmacokinetics of andrographolide dripping pills, a modern chinese herb medicine, by LC-MS/MS method in beagle dogs

A rapid and sensitive method for the analysis of andrographolide in dog plasma using liquid chromatography coupled to tandem electrospray ionization mass spectrometry (LC-MS/MS) was developed and validated. The analyte and internal standard (IS), warfarin, were extracted from plasma with ethyl aceta...

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Detalles Bibliográficos
Autores principales: Chu, Yang, Bai, Xiaolin, Zhang, Shunnan, Li, Wei, Wang, Xiangyang, Guo, Jiahua, Ma, Xiaohui, Zhu, Yonghong
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Andrographolide
Beagle dogs
Dripping pills
LC-MS/MS
Pharmacokinetics
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/25726
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_2_1.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-25726
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Andrographolide
Beagle dogs
Dripping pills
LC-MS/MS
Pharmacokinetics
spellingShingle Farmacia
Andrographolide
Beagle dogs
Dripping pills
LC-MS/MS
Pharmacokinetics
Chu, Yang
Bai, Xiaolin
Zhang, Shunnan
Li, Wei
Wang, Xiangyang
Guo, Jiahua
Ma, Xiaohui
Zhu, Yonghong
Pharmacokinetics of andrographolide dripping pills, a modern chinese herb medicine, by LC-MS/MS method in beagle dogs
topic_facet Farmacia
Andrographolide
Beagle dogs
Dripping pills
LC-MS/MS
Pharmacokinetics
description A rapid and sensitive method for the analysis of andrographolide in dog plasma using liquid chromatography coupled to tandem electrospray ionization mass spectrometry (LC-MS/MS) was developed and validated. The analyte and internal standard (IS), warfarin, were extracted from plasma with ethyl acetate and then separated by RP-HPLC. Detection was performed by negative ion electrospray ionization in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 349.1→287.2 and m/z 307.0→116.9, for quantification of andrographolide and IS, respectively. Excellent linearity was found to be from 2.5 to 1000 ng/mL with a lowest limit of quantitation (LLOQ) of 2.5 ng/mL. The inter-day accuracy of the drug containing plasma samples was 100.4-109.7 %, with a precision of 3.72-6.94 %. The intraday accuracy was 95.1-102.5 %, with a precision of 6.04-9.87 %. The developed method was successfully applied to the pharmacokinetic study of andrographolide dripping pills in Beagle dogs for the first time.
format Articulo
Comunicacion
author Chu, Yang
Bai, Xiaolin
Zhang, Shunnan
Li, Wei
Wang, Xiangyang
Guo, Jiahua
Ma, Xiaohui
Zhu, Yonghong
author_facet Chu, Yang
Bai, Xiaolin
Zhang, Shunnan
Li, Wei
Wang, Xiangyang
Guo, Jiahua
Ma, Xiaohui
Zhu, Yonghong
author_sort Chu, Yang
title Pharmacokinetics of andrographolide dripping pills, a modern chinese herb medicine, by LC-MS/MS method in beagle dogs
title_short Pharmacokinetics of andrographolide dripping pills, a modern chinese herb medicine, by LC-MS/MS method in beagle dogs
title_full Pharmacokinetics of andrographolide dripping pills, a modern chinese herb medicine, by LC-MS/MS method in beagle dogs
title_fullStr Pharmacokinetics of andrographolide dripping pills, a modern chinese herb medicine, by LC-MS/MS method in beagle dogs
title_full_unstemmed Pharmacokinetics of andrographolide dripping pills, a modern chinese herb medicine, by LC-MS/MS method in beagle dogs
title_sort pharmacokinetics of andrographolide dripping pills, a modern chinese herb medicine, by lc-ms/ms method in beagle dogs
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/25726
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_2_1.pdf
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