Simultaneous determination of four cytochrome p450 probe drugs in rat plasma by a simple liquid chromatography–mass spectrometry method

A sensitive and simple liquid chromatography- mass spectrometry (LC-MS) method was developed and validated for determination of four cytochrome P450 probe drugs (phenacetin (CYP1A2), tolbutamide (CYP2C9), bupropion (CYP2B6) and omeprazole (CYP2C19) in rat plasma. Four cytochrome P450 probe drugs ext...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Hu, Lufeng, Xu, Ren-ai, Wang, Zhe, Ma, Jianshe, Pan, Jianchun, Wang, Xianqin
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Bupropion
LC-MS
Omeprazole
Phenacetin
Rat plasma
Tolbutamide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/25724
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_1_15.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-25724
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Bupropion
LC-MS
Omeprazole
Phenacetin
Rat plasma
Tolbutamide
spellingShingle Farmacia
Bupropion
LC-MS
Omeprazole
Phenacetin
Rat plasma
Tolbutamide
Hu, Lufeng
Xu, Ren-ai
Wang, Zhe
Ma, Jianshe
Pan, Jianchun
Wang, Xianqin
Simultaneous determination of four cytochrome p450 probe drugs in rat plasma by a simple liquid chromatography–mass spectrometry method
topic_facet Farmacia
Bupropion
LC-MS
Omeprazole
Phenacetin
Rat plasma
Tolbutamide
description A sensitive and simple liquid chromatography- mass spectrometry (LC-MS) method was developed and validated for determination of four cytochrome P450 probe drugs (phenacetin (CYP1A2), tolbutamide (CYP2C9), bupropion (CYP2B6) and omeprazole (CYP2C19) in rat plasma. Four cytochrome P450 probe drugs extracted from plasma samples by protein precipitation with acetonitrile and separation were carried out on Agilent Zorbax SB-C18 column (2.1 mm x 150 mm, 5 μm) at 30 °C, acetonitrile –0.1 % formic acid in water used as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode, and selective ion monitoring (SIM) mode was used to quantify cytochrome P450 probe drugs. The method showed excellent intra-assay and inter-assay precision (relative standard deviation (RSD) and bias <15 %) for quality control (QC) samples spiked at a concentration of 10, 400, and 1600 ng/mL and the r2 > 0.99 over the range investigated (5-2000 ng/mL). Lower limits of quantification (LLOQs) were estimated to be 5 ng/mL. The method was successfully applied to determinate cytochrome P450 probe drugs in a pharmacokinetic study.
format Articulo
Articulo
author Hu, Lufeng
Xu, Ren-ai
Wang, Zhe
Ma, Jianshe
Pan, Jianchun
Wang, Xianqin
author_facet Hu, Lufeng
Xu, Ren-ai
Wang, Zhe
Ma, Jianshe
Pan, Jianchun
Wang, Xianqin
author_sort Hu, Lufeng
title Simultaneous determination of four cytochrome p450 probe drugs in rat plasma by a simple liquid chromatography–mass spectrometry method
title_short Simultaneous determination of four cytochrome p450 probe drugs in rat plasma by a simple liquid chromatography–mass spectrometry method
title_full Simultaneous determination of four cytochrome p450 probe drugs in rat plasma by a simple liquid chromatography–mass spectrometry method
title_fullStr Simultaneous determination of four cytochrome p450 probe drugs in rat plasma by a simple liquid chromatography–mass spectrometry method
title_full_unstemmed Simultaneous determination of four cytochrome p450 probe drugs in rat plasma by a simple liquid chromatography–mass spectrometry method
title_sort simultaneous determination of four cytochrome p450 probe drugs in rat plasma by a simple liquid chromatography–mass spectrometry method
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/25724
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_1_15.pdf
work_keys_str_mv AT hulufeng simultaneousdeterminationoffourcytochromep450probedrugsinratplasmabyasimpleliquidchromatographymassspectrometrymethod
AT xurenai simultaneousdeterminationoffourcytochromep450probedrugsinratplasmabyasimpleliquidchromatographymassspectrometrymethod
AT wangzhe simultaneousdeterminationoffourcytochromep450probedrugsinratplasmabyasimpleliquidchromatographymassspectrometrymethod
AT majianshe simultaneousdeterminationoffourcytochromep450probedrugsinratplasmabyasimpleliquidchromatographymassspectrometrymethod
AT panjianchun simultaneousdeterminationoffourcytochromep450probedrugsinratplasmabyasimpleliquidchromatographymassspectrometrymethod
AT wangxianqin simultaneousdeterminationoffourcytochromep450probedrugsinratplasmabyasimpleliquidchromatographymassspectrometrymethod
bdutipo_str Repositorios
_version_ 1764820466841681921

Ejemplares similares