Determination of free cyclosporine A with a LC-MS/MS method: Application to C2 monitoring in rabbits

Cyclosporine A (CsA) is a cyclic peptide widely used as an immunosuppressant. Therapeutic drug monitoring (TDM) of CsA is becoming mandatory for transplant patients who received CsA therapy in the routine clinical practice because of large individual variability, dose-related toxicity and the risk o...

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Autores principales: Xu, Ren-ai, Zhang, Chunhong, Pan, Xiaofeng, Shi, Da-wei, Hu, Lufeng, Ye, Xiao-lan, Liu, Yun-jie, Zhang, Xiuhua
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Cyclosporine A
LC-MS/MS
Therapeutic drug monitoring
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/25723
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_1_14.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-25723
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Cyclosporine A
LC-MS/MS
Therapeutic drug monitoring
spellingShingle Farmacia
Cyclosporine A
LC-MS/MS
Therapeutic drug monitoring
Xu, Ren-ai
Zhang, Chunhong
Pan, Xiaofeng
Shi, Da-wei
Hu, Lufeng
Ye, Xiao-lan
Liu, Yun-jie
Zhang, Xiuhua
Determination of free cyclosporine A with a LC-MS/MS method: Application to C2 monitoring in rabbits
topic_facet Farmacia
Cyclosporine A
LC-MS/MS
Therapeutic drug monitoring
description Cyclosporine A (CsA) is a cyclic peptide widely used as an immunosuppressant. Therapeutic drug monitoring (TDM) of CsA is becoming mandatory for transplant patients who received CsA therapy in the routine clinical practice because of large individual variability, dose-related toxicity and the risk of acute rejection. In this study, a rapid, sensitive, and selective LC-MS/MS method was developed and validated for the quantitative analysis of free CsA (fCsA), a better indicator for the prediction of efficacy and safety of CsA-based therapy. Following ultrafiltration for fCsA, chromatographic separation was performed on an Agilent Zorbax SB-C18 column (100 mm x 2.1 mm, 3.5 μm ) with acetonitrile and 0.1 % ammonium hydroxide in water (85:15, v/v) as the mobile phases. The compounds were quantified by positive electrospray ionization tandem mass spectrometry. Selectivity, linearity, accuracy, precision, recovery, and stability were evaluated during method validation. The validated method was applied to a single blood concentration measurement 2 h after CsA administration (C2) measurement study of fCsA after an oral administration of a single 15 mg/kg intravenous dose of CsA to six rabbits.
format Articulo
Articulo
author Xu, Ren-ai
Zhang, Chunhong
Pan, Xiaofeng
Shi, Da-wei
Hu, Lufeng
Ye, Xiao-lan
Liu, Yun-jie
Zhang, Xiuhua
author_facet Xu, Ren-ai
Zhang, Chunhong
Pan, Xiaofeng
Shi, Da-wei
Hu, Lufeng
Ye, Xiao-lan
Liu, Yun-jie
Zhang, Xiuhua
author_sort Xu, Ren-ai
title Determination of free cyclosporine A with a LC-MS/MS method: Application to C2 monitoring in rabbits
title_short Determination of free cyclosporine A with a LC-MS/MS method: Application to C2 monitoring in rabbits
title_full Determination of free cyclosporine A with a LC-MS/MS method: Application to C2 monitoring in rabbits
title_fullStr Determination of free cyclosporine A with a LC-MS/MS method: Application to C2 monitoring in rabbits
title_full_unstemmed Determination of free cyclosporine A with a LC-MS/MS method: Application to C2 monitoring in rabbits
title_sort determination of free cyclosporine a with a lc-ms/ms method: application to c2 monitoring in rabbits
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/25723
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_1_14.pdf
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AT zhangchunhong determinationoffreecyclosporineawithalcmsmsmethodapplicationtoc2monitoringinrabbits
AT panxiaofeng determinationoffreecyclosporineawithalcmsmsmethodapplicationtoc2monitoringinrabbits
AT shidawei determinationoffreecyclosporineawithalcmsmsmethodapplicationtoc2monitoringinrabbits
AT hulufeng determinationoffreecyclosporineawithalcmsmsmethodapplicationtoc2monitoringinrabbits
AT yexiaolan determinationoffreecyclosporineawithalcmsmsmethodapplicationtoc2monitoringinrabbits
AT liuyunjie determinationoffreecyclosporineawithalcmsmsmethodapplicationtoc2monitoringinrabbits
AT zhangxiuhua determinationoffreecyclosporineawithalcmsmsmethodapplicationtoc2monitoringinrabbits
bdutipo_str Repositorios
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