Interaction between Nobiliside-a and lipid bilayers

Nobiliside A (Nob) is a new triterpenoid saponin first discovered and isolated from the Holothuria nobilis with chemical molecular structure of C54H87O26 SNa. Extracorporeal antitumor test showed that Nob may be a new category of effective anticancer medicine which had excellent cytotoxicity as well...

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Autores principales: Xiong, Liyan, Chen, Jianming, Guo, Dan
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Rastreo Diferencial de Calorimetría
Espectroscopía Infrarroja Transformadora de Fourier
Liposomas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/25637
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_1_2.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-25637
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Rastreo Diferencial de Calorimetría
Espectroscopía Infrarroja Transformadora de Fourier
Liposomas
spellingShingle Farmacia
Rastreo Diferencial de Calorimetría
Espectroscopía Infrarroja Transformadora de Fourier
Liposomas
Xiong, Liyan
Chen, Jianming
Guo, Dan
Interaction between Nobiliside-a and lipid bilayers
topic_facet Farmacia
Rastreo Diferencial de Calorimetría
Espectroscopía Infrarroja Transformadora de Fourier
Liposomas
description Nobiliside A (Nob) is a new triterpenoid saponin first discovered and isolated from the Holothuria nobilis with chemical molecular structure of C54H87O26 SNa. Extracorporeal antitumor test showed that Nob may be a new category of effective anticancer medicine which had excellent cytotoxicity as well as inhibited vascular endothelial cell (VEC) proliferation and migration in vitro and chicken chorioallantoic membrane (CAM) angiogenesis in vivo at a lower dose. Unfortunately, the clinical application of Nob was severely limited by the low bioavailability of Nob after oral administration, and highly toxic especially heart toxicity and the ability causing hemolysis of blood cells after intravenous injection. To reduce the hemolysis and toxicity of Nob after intravenous injection, liposomes were used as its carriers and good effect was acquired in our previous study. During the preparation and study of Nob liposomes, we found that Nob liposomes had high encapsulation efficiency (EE), which nearly 100 % and good stability. It was proposed that there would be strong interaction between Nob and lipid bilayers, which would affect the EE, the stability, pharmacokinetics, pharmacodynamics and even the toxicity of the drug. Thus, fourier transformer infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), fluoroscense spectroscopy were used to study the interaction between Nob and lipid bilayers. The results showed that there was a strong interaction between Nob and both phospholipids (PL) and cholesterol (CH) in lipid bilayers, and the interaction between Nob and CH was stronger than that between Nob and PL. There was also interaction between PL and CH, which would be decreased when Nob existed. Thus, the reason of Nob liposomes having high EE and good stability could be infered from the study. In fluoroscense spectroscopy study it was found that Nob could destroy calcein liposomes and lead release of the content, while Nob encapsuled in liposomes could not cause the destruction of calcein liposomes. These phenomena were different with Nob liposomes leading to the content release from red blood cells, so the mechanism of Nob liposomes decreasing the toxicity to mice and hemolysis in vitro should be further studied.
format Articulo
Articulo
author Xiong, Liyan
Chen, Jianming
Guo, Dan
author_facet Xiong, Liyan
Chen, Jianming
Guo, Dan
author_sort Xiong, Liyan
title Interaction between Nobiliside-a and lipid bilayers
title_short Interaction between Nobiliside-a and lipid bilayers
title_full Interaction between Nobiliside-a and lipid bilayers
title_fullStr Interaction between Nobiliside-a and lipid bilayers
title_full_unstemmed Interaction between Nobiliside-a and lipid bilayers
title_sort interaction between nobiliside-a and lipid bilayers
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/25637
http://www.latamjpharm.org/resumenes/31/6/LAJOP_31_6_1_2.pdf
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AT chenjianming interactionbetweennobilisideaandlipidbilayers
AT guodan interactionbetweennobilisideaandlipidbilayers
bdutipo_str Repositorios
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