Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes

This study was performed to screen α-glucosidase inhibitors from the actinomycete metabolites library by high throughput screening. Twelve strains of actinomycete were considered to be α-glucosidase inhibitors producing strains; then effective inhibitory strain PW409 was fermented and separated by b...

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Detalles Bibliográficos
Autores principales: Wang, Liqiang, Hou, Yuan-Yuan, Peng, Jiamin, Qi, Xizhen, Zhang, Qi, Bai, Fang, Bai, Gang
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Actinomycetes
Bioactivity-based HPLC
α-glucosidase inhibitor
Identification
Quantification
Screening
alfa-Glucosidasas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/20220
http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_1_8.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-20220
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Actinomycetes
Bioactivity-based HPLC
α-glucosidase inhibitor
Identification
Quantification
Screening
alfa-Glucosidasas
spellingShingle Farmacia
Actinomycetes
Bioactivity-based HPLC
α-glucosidase inhibitor
Identification
Quantification
Screening
alfa-Glucosidasas
Wang, Liqiang
Hou, Yuan-Yuan
Peng, Jiamin
Qi, Xizhen
Zhang, Qi
Bai, Fang
Bai, Gang
Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
topic_facet Farmacia
Actinomycetes
Bioactivity-based HPLC
α-glucosidase inhibitor
Identification
Quantification
Screening
alfa-Glucosidasas
description This study was performed to screen α-glucosidase inhibitors from the actinomycete metabolites library by high throughput screening. Twelve strains of actinomycete were considered to be α-glucosidase inhibitors producing strains; then effective inhibitory strain PW409 was fermented and separated by bioactivity based HPLC, two fractions showing remarkable inhibitory activities; the two compounds were identified as 1-deoxynojirimycin (DNJ) and miglitol by mass spectrometry, comparing with authentic standards, and relevant literature. The quantification analysis of DNJ and miglitol by HPLC-MS/MS showed that the average concentrations of DNJ and miglitol in broth of strain PW409 were 11.2 and 95.8 mg/L, respectively. This is the first report about Streptomyces sp. products α-glucosidase inhibitor miglitol. The strain PW409 has potential application in biosynthesis and biotransformation of antidiabetes drug miglitol. The method can be utilized for new α-glucosidase inhibitors discovering and development from other inhibitory activity strains.
format Articulo
Articulo
author Wang, Liqiang
Hou, Yuan-Yuan
Peng, Jiamin
Qi, Xizhen
Zhang, Qi
Bai, Fang
Bai, Gang
author_facet Wang, Liqiang
Hou, Yuan-Yuan
Peng, Jiamin
Qi, Xizhen
Zhang, Qi
Bai, Fang
Bai, Gang
author_sort Wang, Liqiang
title Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_short Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_full Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_fullStr Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_full_unstemmed Bioactivity-based HPLC tandem Q/TOF for alpha-glucosidase inhibitors : Screening, identification, and quantification from actinomycetes
title_sort bioactivity-based hplc tandem q/tof for alpha-glucosidase inhibitors : screening, identification, and quantification from actinomycetes
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/20220
http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_1_8.pdf
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bdutipo_str Repositorios
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