Determination of berberine and berberrubine in rabbit plasma by capillary electrophoresis with amperometric detection and its application to pharmacokinetic study
A simple and selective method based on capillary electrophoresis with amperometric detection has been developed for the separation and determination of berberine (BB) and berberrubine (BBR) in rabbit plasma. The working electrode was a 150 μm diameter platinum microelectrode at a detection potential...
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| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/20216 http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_1_4.pdf |
| Aporte de: |
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I19-R120-10915-20216 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia Berberina Conejos Electroforesis Farmacocinética Amperometric detection Berberine Berberrubine Capillary electrophoresis |
| spellingShingle |
Farmacia Berberina Conejos Electroforesis Farmacocinética Amperometric detection Berberine Berberrubine Capillary electrophoresis Deng, Guang-Hui Wang, Hui Gao, Jin Wang, Shiwei Determination of berberine and berberrubine in rabbit plasma by capillary electrophoresis with amperometric detection and its application to pharmacokinetic study |
| topic_facet |
Farmacia Berberina Conejos Electroforesis Farmacocinética Amperometric detection Berberine Berberrubine Capillary electrophoresis |
| description |
A simple and selective method based on capillary electrophoresis with amperometric detection has been developed for the separation and determination of berberine (BB) and berberrubine (BBR) in rabbit plasma. The working electrode was a 150 μm diameter platinum microelectrode at a detection potential of +1.1 V (versus Ag/AgCl). The analytes could be well separated within 6 min in a 50 cm length fused-silica capillary at a separation voltage of 15 kV in a 50 mmol/L phosphate buffer (pH 6.0). Calibration curve was linear over the range of 0.002-20 μg/mL. The detection limits (S/N = 3) were 1.0 and 0.8 ng/mL for BB and BBR, respectively. Relative standard deviations (R.S.D.) of not more than 6.0 % were obtained for both migration times and electrophoretic peak area. The proposed method was successfully used to analyze the drugs in samples of rabbit plasma for pharmacokinetic study. |
| format |
Articulo Articulo |
| author |
Deng, Guang-Hui Wang, Hui Gao, Jin Wang, Shiwei |
| author_facet |
Deng, Guang-Hui Wang, Hui Gao, Jin Wang, Shiwei |
| author_sort |
Deng, Guang-Hui |
| title |
Determination of berberine and berberrubine in rabbit plasma by capillary electrophoresis with amperometric detection and its application to pharmacokinetic study |
| title_short |
Determination of berberine and berberrubine in rabbit plasma by capillary electrophoresis with amperometric detection and its application to pharmacokinetic study |
| title_full |
Determination of berberine and berberrubine in rabbit plasma by capillary electrophoresis with amperometric detection and its application to pharmacokinetic study |
| title_fullStr |
Determination of berberine and berberrubine in rabbit plasma by capillary electrophoresis with amperometric detection and its application to pharmacokinetic study |
| title_full_unstemmed |
Determination of berberine and berberrubine in rabbit plasma by capillary electrophoresis with amperometric detection and its application to pharmacokinetic study |
| title_sort |
determination of berberine and berberrubine in rabbit plasma by capillary electrophoresis with amperometric detection and its application to pharmacokinetic study |
| publishDate |
2012 |
| url |
http://sedici.unlp.edu.ar/handle/10915/20216 http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_1_4.pdf |
| work_keys_str_mv |
AT dengguanghui determinationofberberineandberberrubineinrabbitplasmabycapillaryelectrophoresiswithamperometricdetectionanditsapplicationtopharmacokineticstudy AT wanghui determinationofberberineandberberrubineinrabbitplasmabycapillaryelectrophoresiswithamperometricdetectionanditsapplicationtopharmacokineticstudy AT gaojin determinationofberberineandberberrubineinrabbitplasmabycapillaryelectrophoresiswithamperometricdetectionanditsapplicationtopharmacokineticstudy AT wangshiwei determinationofberberineandberberrubineinrabbitplasmabycapillaryelectrophoresiswithamperometricdetectionanditsapplicationtopharmacokineticstudy |
| bdutipo_str |
Repositorios |
| _version_ |
1764820465478533121 |