Determination of pantoprazole in rat plasma by LC–MS/MS and its application to pharmacokinetics
A highly sensitive liquid chromatographic mass spectrometric (LC-MS/MS) method for determination of pantoprazole in rat plasma using omeprazole as the internal standard (IS) was developed. Plasma samples were precipitated by acetonitrile and separated on a Zorbax SB-C18 column with gradient profile...
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| Autores principales: | , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/20214 http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_1_2.pdf |
| Aporte de: |
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I19-R120-10915-20214 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia LC-MS/MS Pantoprazole Pharmacokinetics Rat plasma Ratas Farmacocinética |
| spellingShingle |
Farmacia LC-MS/MS Pantoprazole Pharmacokinetics Rat plasma Ratas Farmacocinética Qiu, Xiangjun Xu, Ren-ai Zheng, Yuancai Zhan, Haichao Wang, Zhe Hu, Lufeng Determination of pantoprazole in rat plasma by LC–MS/MS and its application to pharmacokinetics |
| topic_facet |
Farmacia LC-MS/MS Pantoprazole Pharmacokinetics Rat plasma Ratas Farmacocinética |
| description |
A highly sensitive liquid chromatographic mass spectrometric (LC-MS/MS) method for determination of pantoprazole in rat plasma using omeprazole as the internal standard (IS) was developed.
Plasma samples were precipitated by acetonitrile and separated on a Zorbax SB-C18 column with gradient profile at a flow of 0.4 mL/min. Detection was carried out by SIM mode on an ion-trap LC-MS/MS system with an electrospray ionization interface. The lower limit of quantification (LLOQ) was 5 ng/mL.
Calibration curve was linear over the range from 5 to 5000 ng/mL. The intra- and inter-run relative standard deviations of the assay were less than 7 %. The mean absolute recoveries determined at the concentrations of 25, 400, and 4000 ng/mL were 87.40 ± 4.40 %, 87.77 ± 3.30 %, and 92.78 ± 5.02 %, respectively.
The method was applied to the pharmacokinetic of 15 mg/kg of pantoprazole in six rats. |
| format |
Articulo Articulo |
| author |
Qiu, Xiangjun Xu, Ren-ai Zheng, Yuancai Zhan, Haichao Wang, Zhe Hu, Lufeng |
| author_facet |
Qiu, Xiangjun Xu, Ren-ai Zheng, Yuancai Zhan, Haichao Wang, Zhe Hu, Lufeng |
| author_sort |
Qiu, Xiangjun |
| title |
Determination of pantoprazole in rat plasma by LC–MS/MS and its application to pharmacokinetics |
| title_short |
Determination of pantoprazole in rat plasma by LC–MS/MS and its application to pharmacokinetics |
| title_full |
Determination of pantoprazole in rat plasma by LC–MS/MS and its application to pharmacokinetics |
| title_fullStr |
Determination of pantoprazole in rat plasma by LC–MS/MS and its application to pharmacokinetics |
| title_full_unstemmed |
Determination of pantoprazole in rat plasma by LC–MS/MS and its application to pharmacokinetics |
| title_sort |
determination of pantoprazole in rat plasma by lc–ms/ms and its application to pharmacokinetics |
| publishDate |
2012 |
| url |
http://sedici.unlp.edu.ar/handle/10915/20214 http://www.latamjpharm.org/resumenes/31/5/LAJOP_31_5_1_2.pdf |
| work_keys_str_mv |
AT qiuxiangjun determinationofpantoprazoleinratplasmabylcmsmsanditsapplicationtopharmacokinetics AT xurenai determinationofpantoprazoleinratplasmabylcmsmsanditsapplicationtopharmacokinetics AT zhengyuancai determinationofpantoprazoleinratplasmabylcmsmsanditsapplicationtopharmacokinetics AT zhanhaichao determinationofpantoprazoleinratplasmabylcmsmsanditsapplicationtopharmacokinetics AT wangzhe determinationofpantoprazoleinratplasmabylcmsmsanditsapplicationtopharmacokinetics AT hulufeng determinationofpantoprazoleinratplasmabylcmsmsanditsapplicationtopharmacokinetics |
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Repositorios |
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1764820465476435970 |