Quantitative determination and pharmacokinetics of salvianolic acid l, a novel phenolic acid constituent from salvia miltiorrhiza, in rat plasma by high-performance liquid chromatography
A simple, rapid and selective HPLC method was developed for the determination of a novel phenolic acid constituent in rat plasma, salvianolic acid L (SAL), extracted from the dried root of Salvia miltiorrhiza (Danshen). Plasma samples were extracted by ethyl acetate after addition of the internal st...
Guardado en:
| Autores principales: | , , , , , , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/18278 http://www.latamjpharm.org/resumenes/31/3/LAJOP_31_3_2_3.pdf |
| Aporte de: |
| id |
I19-R120-10915-18278 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia Farmacología Farmacocinética danshen; HPLC; pharmacokinetics; salvia miltiorrhiza; salvianolic acid L |
| spellingShingle |
Farmacia Farmacología Farmacocinética danshen; HPLC; pharmacokinetics; salvia miltiorrhiza; salvianolic acid L Zhao, Yongyue Chu, Yang Li, Wei Guo, Jiahua Wang, Xiangyang Ma, Xiaohui Jin, Yuanpeng Aa, Jiye Wang, Guangji Quantitative determination and pharmacokinetics of salvianolic acid l, a novel phenolic acid constituent from salvia miltiorrhiza, in rat plasma by high-performance liquid chromatography |
| topic_facet |
Farmacia Farmacología Farmacocinética danshen; HPLC; pharmacokinetics; salvia miltiorrhiza; salvianolic acid L |
| description |
A simple, rapid and selective HPLC method was developed for the determination of a novel phenolic acid constituent in rat plasma, salvianolic acid L (SAL), extracted from the dried root of Salvia miltiorrhiza (Danshen). Plasma samples were extracted by ethyl acetate after addition of the internal standard tinidazole. The appropriate separations were achieved using a C18 column with the mobile phase composed of a mixture of acetonitrile/water/formic acid (35:65:0.1, v/v/v) at the flow rate of 0.8 mL/min, and the wavelength of determination by diode-array detector (DAD) detection was 327 nm. Good linearity (r = 0.9996) was obtained within the concentration of 0.05-50 μg/mL. The intra- and inter-day assay precisions ranged from 0.60 to 5.91% and 3.52 to 7.00%, respectively. The accuracy was between 95.8 to 103.8%. In addition, the stability and extraction recovery involved in the method were also validated. This method was successfully applied to investigate the pharmacokinetic study of SAL in rats after a single intravenous administration dose of 2.0, 4.0, and 8.0 mg/kg, respectively. |
| format |
Articulo Comunicacion |
| author |
Zhao, Yongyue Chu, Yang Li, Wei Guo, Jiahua Wang, Xiangyang Ma, Xiaohui Jin, Yuanpeng Aa, Jiye Wang, Guangji |
| author_facet |
Zhao, Yongyue Chu, Yang Li, Wei Guo, Jiahua Wang, Xiangyang Ma, Xiaohui Jin, Yuanpeng Aa, Jiye Wang, Guangji |
| author_sort |
Zhao, Yongyue |
| title |
Quantitative determination and pharmacokinetics of salvianolic acid l, a novel phenolic acid constituent from salvia miltiorrhiza, in rat plasma by high-performance liquid chromatography |
| title_short |
Quantitative determination and pharmacokinetics of salvianolic acid l, a novel phenolic acid constituent from salvia miltiorrhiza, in rat plasma by high-performance liquid chromatography |
| title_full |
Quantitative determination and pharmacokinetics of salvianolic acid l, a novel phenolic acid constituent from salvia miltiorrhiza, in rat plasma by high-performance liquid chromatography |
| title_fullStr |
Quantitative determination and pharmacokinetics of salvianolic acid l, a novel phenolic acid constituent from salvia miltiorrhiza, in rat plasma by high-performance liquid chromatography |
| title_full_unstemmed |
Quantitative determination and pharmacokinetics of salvianolic acid l, a novel phenolic acid constituent from salvia miltiorrhiza, in rat plasma by high-performance liquid chromatography |
| title_sort |
quantitative determination and pharmacokinetics of salvianolic acid l, a novel phenolic acid constituent from salvia miltiorrhiza, in rat plasma by high-performance liquid chromatography |
| publishDate |
2012 |
| url |
http://sedici.unlp.edu.ar/handle/10915/18278 http://www.latamjpharm.org/resumenes/31/3/LAJOP_31_3_2_3.pdf |
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