Protective Activities of Cistanoside A on CCl<sub>4</sub> Induced Hepatotoxicity in Mice

To evaluate the protective efficacy of Cistanoside A (C.A), a phenylethanol glycoside isolated from Cistanche deserticola, on CCl4 induced hepatotoxicity in mice, 50 animals were divided into five different protocols, and hepatic functional index were detected by diagnostic kits. Histological change...

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Autores principales: Luo, Huiying, Wang, Lijuan, Li, Juan, Zhu, Lijuan
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Farmacología
cistanoside A; CCl4; free radical; hepatoprotection; respiratory chain
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/18256
http://www.latamjpharm.org/resumenes/31/3/LAJOP_31_3_1_9.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-18256
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Farmacología
cistanoside A; CCl4; free radical; hepatoprotection; respiratory chain
spellingShingle Farmacia
Farmacología
cistanoside A; CCl4; free radical; hepatoprotection; respiratory chain
Luo, Huiying
Wang, Lijuan
Li, Juan
Zhu, Lijuan
Protective Activities of Cistanoside A on CCl<sub>4</sub> Induced Hepatotoxicity in Mice
topic_facet Farmacia
Farmacología
cistanoside A; CCl4; free radical; hepatoprotection; respiratory chain
description To evaluate the protective efficacy of Cistanoside A (C.A), a phenylethanol glycoside isolated from Cistanche deserticola, on CCl4 induced hepatotoxicity in mice, 50 animals were divided into five different protocols, and hepatic functional index were detected by diagnostic kits. Histological changes were compared by H&E stain. Activities of mitochondrial antioxidant enzymes (GST, SOD, and CAT) and respiratory marker enzymes (MDH, SDH, NADH dehydrogenase, and cytochrome c oxidases) were measured. To confirm the effect of C.A on free radical, tests on the free radical scavenging activities were also carried out in vitro. We found treatment with C.A (10, 20 mg/kg o.p. for 7 days) could significantly ameliorated the levels of hepatic function indices (AST, ALT, ALP and LDH) (P < 0.05). The biochemical results were also confirmed by histopathological examination. C.A treatment decreased the ballooning degeneration, moderated the hepatocytes apoptosis, and alleviated centrilobular and bridging necrosis which were observed in the CCl4 control group. Following experiments revealed that C.A could increase the activities of mitochondrial antioxidant enzymes (GST, SOD, and CAT) and respiratory marker enzymes (MDH, SDH, NADH dehydrogenase, and cytochrome c oxidases) (P < 0.05). In vitro, C.A exhibited strong scavenging activities for DPPH radical and superoxide anion radical. Our results revealed that C.A possess protective activities on CCl4 induced hepatotoxicity in mice, which was involved with increasing free radicals clearing activities, alleviating lipid-overoxidation damage, and improving respiratory chain function in mitochondria.
format Articulo
Articulo
author Luo, Huiying
Wang, Lijuan
Li, Juan
Zhu, Lijuan
author_facet Luo, Huiying
Wang, Lijuan
Li, Juan
Zhu, Lijuan
author_sort Luo, Huiying
title Protective Activities of Cistanoside A on CCl<sub>4</sub> Induced Hepatotoxicity in Mice
title_short Protective Activities of Cistanoside A on CCl<sub>4</sub> Induced Hepatotoxicity in Mice
title_full Protective Activities of Cistanoside A on CCl<sub>4</sub> Induced Hepatotoxicity in Mice
title_fullStr Protective Activities of Cistanoside A on CCl<sub>4</sub> Induced Hepatotoxicity in Mice
title_full_unstemmed Protective Activities of Cistanoside A on CCl<sub>4</sub> Induced Hepatotoxicity in Mice
title_sort protective activities of cistanoside a on ccl<sub>4</sub> induced hepatotoxicity in mice
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/18256
http://www.latamjpharm.org/resumenes/31/3/LAJOP_31_3_1_9.pdf
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AT lijuan protectiveactivitiesofcistanosideaoncclsub4subinducedhepatotoxicityinmice
AT zhulijuan protectiveactivitiesofcistanosideaoncclsub4subinducedhepatotoxicityinmice
bdutipo_str Repositorios
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