Development and Validation of Liquid Chromatography-Mass Spectrometry Method for Determination of Febuxostat in Rat Plasma and its Application
A selective liquid chromatography-mass spectrometry (LC–MS) method for determination of febuxostat in rat plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agile...
Guardado en:
| Autores principales: | , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/18144 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_4.pdf |
| Aporte de: |
| id |
I19-R120-10915-18144 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia LC-MS; febuxostat; pharmacokinetics;rat plasma Farmacología Farmacocinética |
| spellingShingle |
Farmacia LC-MS; febuxostat; pharmacokinetics;rat plasma Farmacología Farmacocinética Ding, Xitao Zhang, Qingwei Wang, Zhiyi Lin, Guanyang Development and Validation of Liquid Chromatography-Mass Spectrometry Method for Determination of Febuxostat in Rat Plasma and its Application |
| topic_facet |
Farmacia LC-MS; febuxostat; pharmacokinetics;rat plasma Farmacología Farmacocinética |
| description |
A selective liquid chromatography-mass spectrometry (LC–MS) method for determination of febuxostat in rat plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agilent SB-C18 column (2.1 x150 mm, 5 μm) using 0.1% formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragment ions m/z 317 for febuxostat and m/z 326 for midazolam (internal standard, IS). The assay was linear over the range of 10-2000 ng/mL for febuxostat, with a lower limit of quantitation (LLOQ) of 10 ng/mL for febuxostat. Intra- and inter-day RSDs were less than 15% and the accuracies were in the range of 93.8-111.9% for febuxostat. This developed method was successfully applied to determinate of febuxostat in rat plasma for pharmacokinetic study. |
| format |
Articulo Comunicacion |
| author |
Ding, Xitao Zhang, Qingwei Wang, Zhiyi Lin, Guanyang |
| author_facet |
Ding, Xitao Zhang, Qingwei Wang, Zhiyi Lin, Guanyang |
| author_sort |
Ding, Xitao |
| title |
Development and Validation of Liquid Chromatography-Mass Spectrometry Method for Determination of Febuxostat in Rat Plasma and its Application |
| title_short |
Development and Validation of Liquid Chromatography-Mass Spectrometry Method for Determination of Febuxostat in Rat Plasma and its Application |
| title_full |
Development and Validation of Liquid Chromatography-Mass Spectrometry Method for Determination of Febuxostat in Rat Plasma and its Application |
| title_fullStr |
Development and Validation of Liquid Chromatography-Mass Spectrometry Method for Determination of Febuxostat in Rat Plasma and its Application |
| title_full_unstemmed |
Development and Validation of Liquid Chromatography-Mass Spectrometry Method for Determination of Febuxostat in Rat Plasma and its Application |
| title_sort |
development and validation of liquid chromatography-mass spectrometry method for determination of febuxostat in rat plasma and its application |
| publishDate |
2012 |
| url |
http://sedici.unlp.edu.ar/handle/10915/18144 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_4.pdf |
| work_keys_str_mv |
AT dingxitao developmentandvalidationofliquidchromatographymassspectrometrymethodfordeterminationoffebuxostatinratplasmaanditsapplication AT zhangqingwei developmentandvalidationofliquidchromatographymassspectrometrymethodfordeterminationoffebuxostatinratplasmaanditsapplication AT wangzhiyi developmentandvalidationofliquidchromatographymassspectrometrymethodfordeterminationoffebuxostatinratplasmaanditsapplication AT linguanyang developmentandvalidationofliquidchromatographymassspectrometrymethodfordeterminationoffebuxostatinratplasmaanditsapplication |
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Repositorios |
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1764820463862677505 |