Determination of Escitalopram in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry

A rapid (3.0 min) and sensitive (LLOQ 0.5 ng/mL) analytical method for the quantitation of Escitalopram (ETP) in human plasma is described. The method is based on High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) using paroxetine as internal standard (I.S.). Sample prepar...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Sousa, Carlos E.M., Bonifácio, F. N., Bedor, Danilo C. G., Silva, T. M., Ramos, Virna L.S., Souza Filho, J. P., Leal, Leila B., Oliveira, Amanda T. C., Santana, Davi P.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
brazilian volunteers; escitalopram; LC-MS/MS; pharmacokinetics; validation
Farmacología
Farmacocinética
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/18130
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_2.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-18130
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
brazilian volunteers; escitalopram; LC-MS/MS; pharmacokinetics; validation
Farmacología
Farmacocinética
spellingShingle Farmacia
brazilian volunteers; escitalopram; LC-MS/MS; pharmacokinetics; validation
Farmacología
Farmacocinética
Sousa, Carlos E.M.
Bonifácio, F. N.
Bedor, Danilo C. G.
Silva, T. M.
Ramos, Virna L.S.
Souza Filho, J. P.
Leal, Leila B.
Oliveira, Amanda T. C.
Santana, Davi P.
Determination of Escitalopram in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry
topic_facet Farmacia
brazilian volunteers; escitalopram; LC-MS/MS; pharmacokinetics; validation
Farmacología
Farmacocinética
description A rapid (3.0 min) and sensitive (LLOQ 0.5 ng/mL) analytical method for the quantitation of Escitalopram (ETP) in human plasma is described. The method is based on High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) using paroxetine as internal standard (I.S.). Sample preparation involved precipitation extraction with acetonitrile. The chromatographic separation was achieved on a ACE C18 (125 x 4,6 mm) reversed-phase column and a mobile phase containing acetonitrile/water (60:50 v/v, add 0.2 % formic acid), in isocratic conditions. The target analytes were transferred into a triple quadrupole mass spectrometer equipped with an electrospray ionization source for mass detection. The ion transitions selected for MRM detection were: m/z 325.2 > 109.2 and 330.0 > 192.0 for ETP and I.S., respectively. The assay was linear in the concentration range of 0.5-50 ng/mL. The mean recovery for ETP was 97.69 %. Intra- and inter-day precision (R.S.D.) were < 10.5 % and <8.2 %, respectively and the accuracy (R.E.) was in the range ± 12.23 %. The method was successfully applied to a single oral dose pharmacokinetics study in 28 healthy Brazilian human volunteers.
format Articulo
Comunicacion
author Sousa, Carlos E.M.
Bonifácio, F. N.
Bedor, Danilo C. G.
Silva, T. M.
Ramos, Virna L.S.
Souza Filho, J. P.
Leal, Leila B.
Oliveira, Amanda T. C.
Santana, Davi P.
author_facet Sousa, Carlos E.M.
Bonifácio, F. N.
Bedor, Danilo C. G.
Silva, T. M.
Ramos, Virna L.S.
Souza Filho, J. P.
Leal, Leila B.
Oliveira, Amanda T. C.
Santana, Davi P.
author_sort Sousa, Carlos E.M.
title Determination of Escitalopram in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry
title_short Determination of Escitalopram in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry
title_full Determination of Escitalopram in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry
title_fullStr Determination of Escitalopram in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry
title_full_unstemmed Determination of Escitalopram in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry
title_sort determination of escitalopram in human plasma by high performance liquid chromatography-tandem mass spectrometry
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/18130
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_2.pdf
work_keys_str_mv AT sousacarlosem determinationofescitalopraminhumanplasmabyhighperformanceliquidchromatographytandemmassspectrometry
AT bonifaciofn determinationofescitalopraminhumanplasmabyhighperformanceliquidchromatographytandemmassspectrometry
AT bedordanilocg determinationofescitalopraminhumanplasmabyhighperformanceliquidchromatographytandemmassspectrometry
AT silvatm determinationofescitalopraminhumanplasmabyhighperformanceliquidchromatographytandemmassspectrometry
AT ramosvirnals determinationofescitalopraminhumanplasmabyhighperformanceliquidchromatographytandemmassspectrometry
AT souzafilhojp determinationofescitalopraminhumanplasmabyhighperformanceliquidchromatographytandemmassspectrometry
AT lealleilab determinationofescitalopraminhumanplasmabyhighperformanceliquidchromatographytandemmassspectrometry
AT oliveiraamandatc determinationofescitalopraminhumanplasmabyhighperformanceliquidchromatographytandemmassspectrometry
AT santanadavip determinationofescitalopraminhumanplasmabyhighperformanceliquidchromatographytandemmassspectrometry
bdutipo_str Repositorios
_version_ 1764820463851143168

Ejemplares similares