Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application

A selective and sensitive liquid chromatography-mass spectrometry (LC–MS) method for determination of ondansetron in rat plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography...

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Autores principales: Pang, Lingxia, Wang, Qing, Wang, Youpei, Zheng, Meiqin, Wu, Haiya, Wang, Zhiyi
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2012
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/18129
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_1.pdf
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id I19-R120-10915-18129
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Farmacología
LC-MS; ondansetron; pharmacokinetics; rat plasma
Farmacocinética
spellingShingle Farmacia
Farmacología
LC-MS; ondansetron; pharmacokinetics; rat plasma
Farmacocinética
Pang, Lingxia
Wang, Qing
Wang, Youpei
Zheng, Meiqin
Wu, Haiya
Wang, Zhiyi
Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application
topic_facet Farmacia
Farmacología
LC-MS; ondansetron; pharmacokinetics; rat plasma
Farmacocinética
description A selective and sensitive liquid chromatography-mass spectrometry (LC–MS) method for determination of ondansetron in rat plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agilent SB-C18 column (2.1 x 150 mm, 5 μm) using 0.1 % formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and multiple reaction monitoring (MRM) mode was used for quantification of target fragment ions m/z 294.0→169.7 for ondansetron and m/z 326.0→291.0 for midazolam (internal standard, IS). The assay was linear over the range of 5–1000 ng/mL for ondansetron, with a lower limit of quantitation (LLOQ) of 5 ng/mL for ondansetron. Intra- and inter-day precisions were less than 14 % and the accuracies were in the range of 94.7-113.5 % for ondansetron. This developed method was successfully applied for the determination of ondansetron in rat plasma for pharmacokinetic study.
format Articulo
Comunicacion
author Pang, Lingxia
Wang, Qing
Wang, Youpei
Zheng, Meiqin
Wu, Haiya
Wang, Zhiyi
author_facet Pang, Lingxia
Wang, Qing
Wang, Youpei
Zheng, Meiqin
Wu, Haiya
Wang, Zhiyi
author_sort Pang, Lingxia
title Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application
title_short Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application
title_full Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application
title_fullStr Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application
title_full_unstemmed Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application
title_sort development and validation of lc-ms/ms method for determination of ondansetron in rat plasma and its application
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/18129
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_1.pdf
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