Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application
A selective and sensitive liquid chromatography-mass spectrometry (LC–MS) method for determination of ondansetron in rat plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography...
Guardado en:
| Autores principales: | , , , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/18129 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_1.pdf |
| Aporte de: |
| id |
I19-R120-10915-18129 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia Farmacología LC-MS; ondansetron; pharmacokinetics; rat plasma Farmacocinética |
| spellingShingle |
Farmacia Farmacología LC-MS; ondansetron; pharmacokinetics; rat plasma Farmacocinética Pang, Lingxia Wang, Qing Wang, Youpei Zheng, Meiqin Wu, Haiya Wang, Zhiyi Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application |
| topic_facet |
Farmacia Farmacología LC-MS; ondansetron; pharmacokinetics; rat plasma Farmacocinética |
| description |
A selective and sensitive liquid chromatography-mass spectrometry (LC–MS) method for determination of ondansetron in rat plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agilent SB-C18 column (2.1 x 150 mm, 5 μm) using 0.1 % formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and multiple reaction monitoring (MRM) mode was used for quantification of target fragment
ions m/z 294.0→169.7 for ondansetron and m/z 326.0→291.0 for midazolam (internal standard, IS). The
assay was linear over the range of 5–1000 ng/mL for ondansetron, with a lower limit of quantitation
(LLOQ) of 5 ng/mL for ondansetron. Intra- and inter-day precisions were less than 14 % and the accuracies were in the range of 94.7-113.5 % for ondansetron. This developed method was successfully applied
for the determination of ondansetron in rat plasma for pharmacokinetic study. |
| format |
Articulo Comunicacion |
| author |
Pang, Lingxia Wang, Qing Wang, Youpei Zheng, Meiqin Wu, Haiya Wang, Zhiyi |
| author_facet |
Pang, Lingxia Wang, Qing Wang, Youpei Zheng, Meiqin Wu, Haiya Wang, Zhiyi |
| author_sort |
Pang, Lingxia |
| title |
Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application |
| title_short |
Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application |
| title_full |
Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application |
| title_fullStr |
Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application |
| title_full_unstemmed |
Development and Validation of LC-MS/MS Method for Determination of Ondansetron in rat Plasma and its Application |
| title_sort |
development and validation of lc-ms/ms method for determination of ondansetron in rat plasma and its application |
| publishDate |
2012 |
| url |
http://sedici.unlp.edu.ar/handle/10915/18129 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_2_1.pdf |
| work_keys_str_mv |
AT panglingxia developmentandvalidationoflcmsmsmethodfordeterminationofondansetroninratplasmaanditsapplication AT wangqing developmentandvalidationoflcmsmsmethodfordeterminationofondansetroninratplasmaanditsapplication AT wangyoupei developmentandvalidationoflcmsmsmethodfordeterminationofondansetroninratplasmaanditsapplication AT zhengmeiqin developmentandvalidationoflcmsmsmethodfordeterminationofondansetroninratplasmaanditsapplication AT wuhaiya developmentandvalidationoflcmsmsmethodfordeterminationofondansetroninratplasmaanditsapplication AT wangzhiyi developmentandvalidationoflcmsmsmethodfordeterminationofondansetroninratplasmaanditsapplication |
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Repositorios |
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