New alternative analytical to quantify the anti-leprosy drug dapsone by UV spectrophotometry

Dapsone is the drug of choice for leprosy treatment. Despite the existence of previous analytical methods, this study aimed to develop a new analytical alternative for dapsone quantification by UV spectrophotometry. Since the pharmacopeial method uses methanol, a toxic solvent, the method was develo...

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Autores principales: Araujo, Rian F. M., Coelho, Mayara L., Soares, Mônica F.L.R., Rolim, Larissa A., Neto, Pedro, Soares Sobrinho, José L.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/18101
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_14.pdf
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id I19-R120-10915-18101
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
analytical method; dapsone; UV spectrophotometry.
Infecciones Bacterianas y Micosis
Infecciones por Mycobacterium
Dapsona
Lepra
spellingShingle Farmacia
analytical method; dapsone; UV spectrophotometry.
Infecciones Bacterianas y Micosis
Infecciones por Mycobacterium
Dapsona
Lepra
Araujo, Rian F. M.
Coelho, Mayara L.
Soares, Mônica F.L.R.
Rolim, Larissa A.
Neto, Pedro
Soares Sobrinho, José L.
New alternative analytical to quantify the anti-leprosy drug dapsone by UV spectrophotometry
topic_facet Farmacia
analytical method; dapsone; UV spectrophotometry.
Infecciones Bacterianas y Micosis
Infecciones por Mycobacterium
Dapsona
Lepra
description Dapsone is the drug of choice for leprosy treatment. Despite the existence of previous analytical methods, this study aimed to develop a new analytical alternative for dapsone quantification by UV spectrophotometry. Since the pharmacopeial method uses methanol, a toxic solvent, the method was developed using first dilution in ethanol (500 μg/mL) and second dilution in water (5 μg/mL), with quantification in 295 nm. For validation, the specificity was confirmed by quantification of degraded samples using the developed method in comparison with a second analytical technique (HPLC-DAD). The proposed method proved to be specific, linear, precise, accurate and robust, with analytical low cost and toxicity, besides presenting operational ease.
format Articulo
Articulo
author Araujo, Rian F. M.
Coelho, Mayara L.
Soares, Mônica F.L.R.
Rolim, Larissa A.
Neto, Pedro
Soares Sobrinho, José L.
author_facet Araujo, Rian F. M.
Coelho, Mayara L.
Soares, Mônica F.L.R.
Rolim, Larissa A.
Neto, Pedro
Soares Sobrinho, José L.
author_sort Araujo, Rian F. M.
title New alternative analytical to quantify the anti-leprosy drug dapsone by UV spectrophotometry
title_short New alternative analytical to quantify the anti-leprosy drug dapsone by UV spectrophotometry
title_full New alternative analytical to quantify the anti-leprosy drug dapsone by UV spectrophotometry
title_fullStr New alternative analytical to quantify the anti-leprosy drug dapsone by UV spectrophotometry
title_full_unstemmed New alternative analytical to quantify the anti-leprosy drug dapsone by UV spectrophotometry
title_sort new alternative analytical to quantify the anti-leprosy drug dapsone by uv spectrophotometry
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/18101
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_14.pdf
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