Peroxynitrite-Induced Apoptosis in FaDu Cells is Correlated with the Up-Regulation of PDCD4 Gene

Peroxynitrite (ONOO-) is a highly reactive species that attacks a range of biological targets. The present study was designed to investigate the effect of ONOOon FaDu cells, a human hypopharyngeal cancer cell line, with special attention given to the PDCD4 gene expression in response to this oxidati...

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Detalles Bibliográficos
Autores principales: Liu, Wenwen, Bai, Xiaohui, Wang, Haibo, Li, Jianfeng, Song, Shasha, He, Mingqiang, Zhang, Guodong, Li, Lin, Yue, Zhiyong
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Farmacología
Antibacterial activity; Carboxamide; Oxazolopyrimidine; QSAR; Thiazolopyrimidine
Agentes Antibacterianos
Agentes Antiinfecciosos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/18092
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_7.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-18092
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Farmacología
Antibacterial activity; Carboxamide; Oxazolopyrimidine; QSAR; Thiazolopyrimidine
Agentes Antibacterianos
Agentes Antiinfecciosos
spellingShingle Farmacia
Farmacología
Antibacterial activity; Carboxamide; Oxazolopyrimidine; QSAR; Thiazolopyrimidine
Agentes Antibacterianos
Agentes Antiinfecciosos
Liu, Wenwen
Bai, Xiaohui
Wang, Haibo
Li, Jianfeng
Song, Shasha
He, Mingqiang
Zhang, Guodong
Li, Lin
Yue, Zhiyong
Peroxynitrite-Induced Apoptosis in FaDu Cells is Correlated with the Up-Regulation of PDCD4 Gene
topic_facet Farmacia
Farmacología
Antibacterial activity; Carboxamide; Oxazolopyrimidine; QSAR; Thiazolopyrimidine
Agentes Antibacterianos
Agentes Antiinfecciosos
description Peroxynitrite (ONOO-) is a highly reactive species that attacks a range of biological targets. The present study was designed to investigate the effect of ONOOon FaDu cells, a human hypopharyngeal cancer cell line, with special attention given to the PDCD4 gene expression in response to this oxidative stress. The in vitro cultured FaDu cells were subjected to various concentrations of ONOO-, then, the cell viability and morphological changes were examined by MTT assay and acridine orange staining, respectively. The protein expressions of Caspase-9, Caspase-3, and PDCD4 were determined by western blot and the mRNA expression of PDCD4 was analyzed by RT-PCR. This work demonstrated that ONOOcould inhibit the proliferation and induce apoptosis of FaDu cells. The protein expressions of Caspase-9, Caspase-3, and PDCD4 were up-regulated and, meanwhile, the mRNA expression of PDCD4 was increased, in response to ONOO-. These data suggest that ONOOcan effectively suppress proliferation of FaDu cells via triggering the apoptotic pathway. PDCD4 gene may play an important role in ONOO--induced apoptosis in FaDu cells, which may offer a new target for the treatment of hypopharyngeal carcinoma.
format Articulo
Articulo
author Liu, Wenwen
Bai, Xiaohui
Wang, Haibo
Li, Jianfeng
Song, Shasha
He, Mingqiang
Zhang, Guodong
Li, Lin
Yue, Zhiyong
author_facet Liu, Wenwen
Bai, Xiaohui
Wang, Haibo
Li, Jianfeng
Song, Shasha
He, Mingqiang
Zhang, Guodong
Li, Lin
Yue, Zhiyong
author_sort Liu, Wenwen
title Peroxynitrite-Induced Apoptosis in FaDu Cells is Correlated with the Up-Regulation of PDCD4 Gene
title_short Peroxynitrite-Induced Apoptosis in FaDu Cells is Correlated with the Up-Regulation of PDCD4 Gene
title_full Peroxynitrite-Induced Apoptosis in FaDu Cells is Correlated with the Up-Regulation of PDCD4 Gene
title_fullStr Peroxynitrite-Induced Apoptosis in FaDu Cells is Correlated with the Up-Regulation of PDCD4 Gene
title_full_unstemmed Peroxynitrite-Induced Apoptosis in FaDu Cells is Correlated with the Up-Regulation of PDCD4 Gene
title_sort peroxynitrite-induced apoptosis in fadu cells is correlated with the up-regulation of pdcd4 gene
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/18092
http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_7.pdf
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AT baixiaohui peroxynitriteinducedapoptosisinfaducellsiscorrelatedwiththeupregulationofpdcd4gene
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bdutipo_str Repositorios
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