Determination of phillyrin in rat plasma by high performance liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study
A simple, fast and sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC–MS) method was developed and validated to quantitatively determinate phillyrin in rat plasma using reserpine as internal standard (IS). The sample preparation method was simple only using 0.6 mL aceton...
Guardado en:
| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/18081 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_5.pdf |
| Aporte de: |
| id |
I19-R120-10915-18081 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia HPLC–MS; pharmacokinetic study; phillyrin Cromatografía Líquida de Alta Presión Farmacocinética |
| spellingShingle |
Farmacia HPLC–MS; pharmacokinetic study; phillyrin Cromatografía Líquida de Alta Presión Farmacocinética Cao, Huihui Zhang, Lei Peng, Jingdong Determination of phillyrin in rat plasma by high performance liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study |
| topic_facet |
Farmacia HPLC–MS; pharmacokinetic study; phillyrin Cromatografía Líquida de Alta Presión Farmacocinética |
| description |
A simple, fast and sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC–MS) method was developed and validated to quantitatively determinate phillyrin in rat plasma using reserpine as internal standard (IS). The sample preparation method was simple only using 0.6 mL acetonitrile as protein precipitant, the supernatant was dried at 50 ºC in a vacuum drying oven and then dissolved in 1.0 mL of organic phase consisted with methanol and acetonitrile (1:9, v/v). The analytical samples were separated on a reversed-phase C18 column (150 × 4.6 mm, 5 μm Phenomenex, CA,
USA) at 30 °C, using 0.05 % acetic acid aqueous solution and acetonitrile (56:44, v/v) as mobile phase, at a flow rate of 0.3 mL/min. The detection was performed by electrospray ionization (ESI) mass spectrometry using extracted ion chromatogram (EIC) detection in positive ion mode while monitoring target molecular ions at [M+Na]+ m/z 557.57 for phillyrin and [M+H]+ m/z 609.45 for the I.S. Good linearity was generated over the concentration range of 0.069-8.97 μg/mL (r = 0.9995) with the limit of detection and quantification of 7.04 and 21.12 ng/mL, respectively. Intra-day and inter-day precisions were less than 6 % and the mean recoveries at three tested concentrations ranged from 96.27 to 100.63 %. Those results indicated that the proposed method was fast, sensitive and feasible enough to successfully apply to the pharmacokinetic study in rats after intravenous injection of phillyrin. |
| format |
Articulo Articulo |
| author |
Cao, Huihui Zhang, Lei Peng, Jingdong |
| author_facet |
Cao, Huihui Zhang, Lei Peng, Jingdong |
| author_sort |
Cao, Huihui |
| title |
Determination of phillyrin in rat plasma by high performance liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study |
| title_short |
Determination of phillyrin in rat plasma by high performance liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study |
| title_full |
Determination of phillyrin in rat plasma by high performance liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study |
| title_fullStr |
Determination of phillyrin in rat plasma by high performance liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study |
| title_full_unstemmed |
Determination of phillyrin in rat plasma by high performance liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study |
| title_sort |
determination of phillyrin in rat plasma by high performance liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study |
| publishDate |
2012 |
| url |
http://sedici.unlp.edu.ar/handle/10915/18081 http://www.latamjpharm.org/resumenes/31/2/LAJOP_31_2_1_5.pdf |
| work_keys_str_mv |
AT caohuihui determinationofphillyrininratplasmabyhighperformanceliquidchromatographytandemmassspectrometryanditsapplicationtoapharmacokineticstudy AT zhanglei determinationofphillyrininratplasmabyhighperformanceliquidchromatographytandemmassspectrometryanditsapplicationtoapharmacokineticstudy AT pengjingdong determinationofphillyrininratplasmabyhighperformanceliquidchromatographytandemmassspectrometryanditsapplicationtoapharmacokineticstudy |
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Repositorios |
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