Ctrl-X, Ctrl-C and Ctrl-V in Veterinary Dermatology: skin microbiota transplantation as a promising approach for dogs with cutaneous adverse food reactions

The probability of influencing the skin microbiome for addressing skin disorders opens a novel aisle of therapy. This study aimed to evaluate the efficacy of skin microbiota transplantation (sMt) for cutaneous adverse food reactions (caFr) in dogs. Ten client-owned dogs with caFr were included in th...

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Autores principales: Ural, Kerem, Erdoğan, Hasan, Erdoğan, Songül
Formato: Articulo
Lenguaje:Inglés
Publicado: 2024
Materias:
Ciencias Veterinarias
atopic dermatitis,
food intolerance
microbiota
skin
transfer
dermatitis atópica
intolerancia alimentaria
piel
transferencia
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/178267
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-178267
record_format dspace
spelling I19-R120-10915-1782672025-04-16T20:05:13Z http://sedici.unlp.edu.ar/handle/10915/178267 Ctrl-X, Ctrl-C and Ctrl-V in Veterinary Dermatology: skin microbiota transplantation as a promising approach for dogs with cutaneous adverse food reactions Ctrl-X, Ctrl-C y Ctrl-V en Dermatología Veterinaria: el trasplante de microbiota cutánea como un enfoque prometedor para perros con reacciones cutáneas adversas a los alimentos Ural, Kerem Erdoğan, Hasan Erdoğan, Songül 2024-04-11 2025-04-16T17:53:54Z en Ciencias Veterinarias atopic dermatitis, food intolerance microbiota skin transfer dermatitis atópica intolerancia alimentaria piel transferencia The probability of influencing the skin microbiome for addressing skin disorders opens a novel aisle of therapy. This study aimed to evaluate the efficacy of skin microbiota transplantation (sMt) for cutaneous adverse food reactions (caFr) in dogs. Ten client-owned dogs with caFr were included in the study. Unenriched heterologous sMt was performed using Nivea Skin Refining Clear-Up Strips (N-cUs). The bacterial microbiota of skin samples was analysed by next-generation sequencing of the 16S rRNA gene. Other relevant biomarkers were involved in VAS (visual analogue scale of pruritus score), CADESI-04 (canine atopic dermatitis extent and severity index) and epidermal corneometric analysis. Increased Faecalibacterium (0 to 1.9 %), Peptoclostridium (5.49 % to 9.11 %) and Collinsella (0.65 % to 8.91 %), and decreased Fusobacterium (19.16 % to 9.06 %), Porphyromona (8.75 % to 0.13 %), Streptococcus (1.63 % to 0.14 %) and Staphylococcus (1.09 % to 0.49 %) was evidenced before and after sMt, respectively. Treatment with sMt effectively controlled clinical signs and drastically reduced median VAS pruritus (6.5 vs. 2) and CADESI-04 scores (74.50±22.62 to 19.30±11.30) (p<0,001). In addition, skin pH and hydration values were improved (p<0,001) after sMt. The heterologous and unenriched sMt with N-cUs could be responsible for the clinical recovery observed in this study. La posibilidad de influir en el microbioma cutáneo para tratar trastornos de la piel abre una nueva vía de terapéutica. El objetivo del presente trabajo fue evaluar la eficacia del trasplante de microbiota cutánea (sMt) para establecer las reacciones cutáneas adversas a los alimentos (caFr) en perros. Se realizó un sMt heterólogo no enriquecido utilizando tiras Nivea Skin Refining Clear-Up Strips (N-cUs). La microbiota bacteriana de las muestras de piel fue analizada mediante secuenciación de próxima generación del gen 16S rRNA. Otros biomarcadores relevantes permitieron establecer la puntuación del prurito (VAS), CADESI-04 y el análisis corneométrico epidérmico (hyidratación y pH). El gen 16S rRNA clasificó con éxito la presencia de un aumento de Faecalibacterium (0 a 1,9%), Peptoclostridium (5,49 % a 9,11 %) y Collinsella (0,65 % a 8,91 %), mientras que se observó una disminución de Fusobacterium (19,16 % a 9,06 %), Porphyromonas (8,75 % a 0,13 %), Streptococcus (1,63 % a 0,14 %) y Staphylococcus (1,09 % a 0,49 %), antes y después del sMt. El tratamiento con sMt controló eficazmente los signos clínicos y redujo significativamente la mediana del prurito VAS (6,5 vs. 2) y las puntuaciones CADESI-04 (74,50±22,62 a 19,30±11,30) (p<0,001). Además, los valores de pH e hidratación de la piel mejoraron (p<0,001) después de la sMt. La sMt heteróloga y no enriquecida con N-cUs podría ser responsable de la recuperación clínica observada en este estudio. Facultad de Ciencias Veterinarias Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
atopic dermatitis,
food intolerance
microbiota
skin
transfer
dermatitis atópica
intolerancia alimentaria
piel
transferencia
spellingShingle Ciencias Veterinarias
atopic dermatitis,
food intolerance
microbiota
skin
transfer
dermatitis atópica
intolerancia alimentaria
piel
transferencia
Ural, Kerem
Erdoğan, Hasan
Erdoğan, Songül
Ctrl-X, Ctrl-C and Ctrl-V in Veterinary Dermatology: skin microbiota transplantation as a promising approach for dogs with cutaneous adverse food reactions
topic_facet Ciencias Veterinarias
atopic dermatitis,
food intolerance
microbiota
skin
transfer
dermatitis atópica
intolerancia alimentaria
piel
transferencia
description The probability of influencing the skin microbiome for addressing skin disorders opens a novel aisle of therapy. This study aimed to evaluate the efficacy of skin microbiota transplantation (sMt) for cutaneous adverse food reactions (caFr) in dogs. Ten client-owned dogs with caFr were included in the study. Unenriched heterologous sMt was performed using Nivea Skin Refining Clear-Up Strips (N-cUs). The bacterial microbiota of skin samples was analysed by next-generation sequencing of the 16S rRNA gene. Other relevant biomarkers were involved in VAS (visual analogue scale of pruritus score), CADESI-04 (canine atopic dermatitis extent and severity index) and epidermal corneometric analysis. Increased Faecalibacterium (0 to 1.9 %), Peptoclostridium (5.49 % to 9.11 %) and Collinsella (0.65 % to 8.91 %), and decreased Fusobacterium (19.16 % to 9.06 %), Porphyromona (8.75 % to 0.13 %), Streptococcus (1.63 % to 0.14 %) and Staphylococcus (1.09 % to 0.49 %) was evidenced before and after sMt, respectively. Treatment with sMt effectively controlled clinical signs and drastically reduced median VAS pruritus (6.5 vs. 2) and CADESI-04 scores (74.50±22.62 to 19.30±11.30) (p<0,001). In addition, skin pH and hydration values were improved (p<0,001) after sMt. The heterologous and unenriched sMt with N-cUs could be responsible for the clinical recovery observed in this study.
format Articulo
Articulo
author Ural, Kerem
Erdoğan, Hasan
Erdoğan, Songül
author_facet Ural, Kerem
Erdoğan, Hasan
Erdoğan, Songül
author_sort Ural, Kerem
title Ctrl-X, Ctrl-C and Ctrl-V in Veterinary Dermatology: skin microbiota transplantation as a promising approach for dogs with cutaneous adverse food reactions
title_short Ctrl-X, Ctrl-C and Ctrl-V in Veterinary Dermatology: skin microbiota transplantation as a promising approach for dogs with cutaneous adverse food reactions
title_full Ctrl-X, Ctrl-C and Ctrl-V in Veterinary Dermatology: skin microbiota transplantation as a promising approach for dogs with cutaneous adverse food reactions
title_fullStr Ctrl-X, Ctrl-C and Ctrl-V in Veterinary Dermatology: skin microbiota transplantation as a promising approach for dogs with cutaneous adverse food reactions
title_full_unstemmed Ctrl-X, Ctrl-C and Ctrl-V in Veterinary Dermatology: skin microbiota transplantation as a promising approach for dogs with cutaneous adverse food reactions
title_sort ctrl-x, ctrl-c and ctrl-v in veterinary dermatology: skin microbiota transplantation as a promising approach for dogs with cutaneous adverse food reactions
publishDate 2024
url http://sedici.unlp.edu.ar/handle/10915/178267
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