Insulin-like growth factors in cat placenta
Placental development involves maternal-fetal signalling events, and it is an under-researched topic in feline reproduction. Few studies of endotheliochorial placentas deal with insulin-like growth factors (IGFs), which are among the principal developmental regulators. While placental expression of...
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2024
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/178262 |
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I19-R120-10915-1782622025-04-16T20:05:16Z http://sedici.unlp.edu.ar/handle/10915/178262 Insulin-like growth factors in cat placenta Factores de crecimiento similares a insulina en la placenta de la gata Hernández, Rocío Gomez Castro, María Gimena Rodriguez, Fernanda Casas, Luciano Agustín Portiansky, Enrique Leo Rey, Florencia Barbeito, Claudio Gustavo Diessler, Mónica Elizabeth 2024-04-11 2025-04-16T17:34:58Z en Ciencias Veterinarias gata placenta factores de crecimiento similares a la insulina células trofoblásticas inmunohostoquímica cat placenta insulin-like growth factors trophoblastic cells immunohistochemistry Placental development involves maternal-fetal signalling events, and it is an under-researched topic in feline reproduction. Few studies of endotheliochorial placentas deal with insulin-like growth factors (IGFs), which are among the principal developmental regulators. While placental expression of IGFs and type 1 IGF receptor (IGF1R) has already been reported in bitches, data regarding the IGF system in queens are limited to uterine tissues. This study aimed to detect IGF1, IGF2, and IGF1R in fetal and maternal placental structures. Samples from twenty-three placentas were classified into one of two groups depending on gestational age (G1: ≤43 d.p.c; G2: ≥44 d.p.c) and processed by indirect immunohistochemistry. Labelling with all the antibodies was stronger in the endometrial glands of earlier placentas than in later ones. Maternal endothelium was moderate to strongly labelled, with a decreasing intensity in the endometrium, while the opposite occurred in the labyrinth. Cytotrophoblast cells were more strongly labelled than the syncytiotrophoblast. IGF1 and IGF1R-positive cells were more abundant in decidual cells of later placentas. These results support that the IGF system plays a central role during gestation and development in cats. As far as we know, this is the first report recording immunohistochemical IGFs/IGF1R detection in fetal regions of the feline placenta. La señalización materno-fetal durante el desarrollo placentario es un tema poco investigado en reproducción felina. Los factores de crecimiento similares a insulina (IGF) son importantes reguladores del desarrollo, escasamente estudiados en placentas endoteliocoriales. La expresión placentaria de IGFs y del receptor IGF tipo 1 (IGFR1) ya se ha descrito en perras; en gatas solo se han descrito en el útero. El objetivo de este estudio fue detectar IGF1, IGF2 e IGFR1 en estructuras placentarias fetales y maternas. Veintitrés muestras de placentas fueron clasificadas en dos grupos, según la edad gestacional (G1: ≤43 d.p.c; G2: ≥44 d.p.c); las mismas se procesaron mediante inmunohistoquímica indirecta. La marcación con todos los anticuerpos fue más intensa en las glándulas endometriales de placentas tempranas que en tardías. El endotelio materno se marcó de manera moderada a fuerte, con intensidad decreciente hacia los vasos del endometrio. Las células del citotrofoblasto se marcaron más que el sincitiotrofoblasto. IGF1 e IGF1R fueron más abundantes en las células deciduales de las placentas tardías. Estos resultados permiten sostener la centralidad del sistema IGF durante el desarrollo placentario felino. Según nuestro conocimiento, este es el primer trabajo que informa y describe la detección inmunohistoquímica de IGFs/IGFR1 en regiones fetales de la placenta felina. Facultad de Ciencias Veterinarias Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Veterinarias gata placenta factores de crecimiento similares a la insulina células trofoblásticas inmunohostoquímica cat placenta insulin-like growth factors trophoblastic cells immunohistochemistry |
| spellingShingle |
Ciencias Veterinarias gata placenta factores de crecimiento similares a la insulina células trofoblásticas inmunohostoquímica cat placenta insulin-like growth factors trophoblastic cells immunohistochemistry Hernández, Rocío Gomez Castro, María Gimena Rodriguez, Fernanda Casas, Luciano Agustín Portiansky, Enrique Leo Rey, Florencia Barbeito, Claudio Gustavo Diessler, Mónica Elizabeth Insulin-like growth factors in cat placenta |
| topic_facet |
Ciencias Veterinarias gata placenta factores de crecimiento similares a la insulina células trofoblásticas inmunohostoquímica cat placenta insulin-like growth factors trophoblastic cells immunohistochemistry |
| description |
Placental development involves maternal-fetal signalling events, and it is an under-researched topic in feline reproduction. Few studies of endotheliochorial placentas deal with insulin-like growth factors (IGFs), which are among the principal developmental regulators. While placental expression of IGFs and type 1 IGF receptor (IGF1R) has already been reported in bitches, data regarding the IGF system in queens are limited to uterine tissues. This study aimed to detect IGF1, IGF2, and IGF1R in fetal and maternal placental structures. Samples from twenty-three placentas were classified into one of two groups depending on gestational age (G1: ≤43 d.p.c; G2: ≥44 d.p.c) and processed by indirect immunohistochemistry. Labelling with all the antibodies was stronger in the endometrial glands of earlier placentas than in later ones. Maternal endothelium was moderate to strongly labelled, with a decreasing intensity in the endometrium, while the opposite occurred in the labyrinth. Cytotrophoblast cells were more strongly labelled than the syncytiotrophoblast. IGF1 and IGF1R-positive cells were more abundant in decidual cells of later placentas. These results support that the IGF system plays a central role during gestation and development in cats. As far as we know, this is the first report recording immunohistochemical IGFs/IGF1R detection in fetal regions of the feline placenta. |
| format |
Articulo Articulo |
| author |
Hernández, Rocío Gomez Castro, María Gimena Rodriguez, Fernanda Casas, Luciano Agustín Portiansky, Enrique Leo Rey, Florencia Barbeito, Claudio Gustavo Diessler, Mónica Elizabeth |
| author_facet |
Hernández, Rocío Gomez Castro, María Gimena Rodriguez, Fernanda Casas, Luciano Agustín Portiansky, Enrique Leo Rey, Florencia Barbeito, Claudio Gustavo Diessler, Mónica Elizabeth |
| author_sort |
Hernández, Rocío |
| title |
Insulin-like growth factors in cat placenta |
| title_short |
Insulin-like growth factors in cat placenta |
| title_full |
Insulin-like growth factors in cat placenta |
| title_fullStr |
Insulin-like growth factors in cat placenta |
| title_full_unstemmed |
Insulin-like growth factors in cat placenta |
| title_sort |
insulin-like growth factors in cat placenta |
| publishDate |
2024 |
| url |
http://sedici.unlp.edu.ar/handle/10915/178262 |
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