Supplementary material for "Interplay Of Virulence Factors and Signaling Molecules: Albumin And Calcium-Mediated Biofilm Regulation In Bordetella bronchiseptia"

Bordetella bronchiseptica, a respiratory pathogen capable of infecting various mammals, including humans, is associated with chronic infections, contrasting with the acute infections caused by B. pertussis. Both pathogens can form biofilm-like structures in vivo, providing tolerance against environm...

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Autores principales: Mugni, Sabrina Laura, Ambrosis, Nicolás Martín, O'Toole, George A., Sisti, Federico, Fernández, Julieta
Formato: Conjunto de datos
Lenguaje:Inglés
Publicado: 2025
Materias:
Ciencias Exactas
https://purl.org/becyt/ford/1.6
Bordetella
Biofilm
Interplay
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/177239
https://doi.org/10.35537/10915/177239
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-177239
record_format dspace
spelling I19-R120-10915-1772392025-03-10T20:11:41Z http://sedici.unlp.edu.ar/handle/10915/177239 https://doi.org/10.35537/10915/177239 Supplementary material for "Interplay Of Virulence Factors and Signaling Molecules: Albumin And Calcium-Mediated Biofilm Regulation In Bordetella bronchiseptia" Mugni, Sabrina Laura Ambrosis, Nicolás Martín O'Toole, George A. Sisti, Federico Fernández, Julieta 2025-03-10T18:10:10Z 2025 en Ciencias Exactas https://purl.org/becyt/ford/1.6 Bordetella Biofilm Interplay Bordetella bronchiseptica, a respiratory pathogen capable of infecting various mammals, including humans, is associated with chronic infections, contrasting with the acute infections caused by B. pertussis. Both pathogens can form biofilm-like structures in vivo, providing tolerance against environmental stresses. Biofilm formation in B. bronchiseptica is regulated by the BvgAS two-component system, with intermediate concentrations of certain modulators inducing a phase favoring biofilm formation. Recent studies have highlighted the role of cyclic diguanylate monophosphate (c-di-GMP) in this process: elevated c-di-GMP levels stimulate biofilm formation, whereas phosphodiesterase (PDE) activation reduces biofilms. Respiratory secretions, which contain albumin and calcium at higher concentrations than standard growth media, promote an increase in the amount and localization of the adenylate cyclase toxin (AC), an important Bordetella virulence factor. Secreted AC present in the extracellular media or attached to the outer membrane inhibits biofilm formation. Based on these observations, we hypothesized that serum albumin and calcium inhibit biofilm formation and explored the involvement of c-di-GMP in this process. Our findings demonstrate that albumin and calcium inhibit B. bronchiseptica biofilm formation by two apparently independent mechanisms, formation, increasing with AC secretion playing a significant role, while the filamentous hemagglutinin adhesin (FHA) does not appear to be directly involved. Furthermore,and inducing c-di-GMP degradation. regulates biofilm formation in response to albumin and calcium. This study enhances our understanding of the complex mechanisms governing B. bronchiseptica biofilm formation and its modulation by host factors. Fil: Mugni, Sabrina Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Argentina. Fil: Ambrosis, Nicolás Martín. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Argentina. Fil: O'Toole, George A. Geisel School of Medicine at Dartmouth, Hanover. Department of Microbiology and Immunology. Estados Unidos. Fil: Sisti, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Argentina. Fil: Fernández, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Argentina. Instituto de Biotecnología y Biología Molecular Conjunto de datos Conjunto de datos http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
https://purl.org/becyt/ford/1.6
Bordetella
Biofilm
Interplay
spellingShingle Ciencias Exactas
https://purl.org/becyt/ford/1.6
Bordetella
Biofilm
Interplay
Mugni, Sabrina Laura
Ambrosis, Nicolás Martín
O'Toole, George A.
Sisti, Federico
Fernández, Julieta
Supplementary material for "Interplay Of Virulence Factors and Signaling Molecules: Albumin And Calcium-Mediated Biofilm Regulation In Bordetella bronchiseptia"
topic_facet Ciencias Exactas
https://purl.org/becyt/ford/1.6
Bordetella
Biofilm
Interplay
description Bordetella bronchiseptica, a respiratory pathogen capable of infecting various mammals, including humans, is associated with chronic infections, contrasting with the acute infections caused by B. pertussis. Both pathogens can form biofilm-like structures in vivo, providing tolerance against environmental stresses. Biofilm formation in B. bronchiseptica is regulated by the BvgAS two-component system, with intermediate concentrations of certain modulators inducing a phase favoring biofilm formation. Recent studies have highlighted the role of cyclic diguanylate monophosphate (c-di-GMP) in this process: elevated c-di-GMP levels stimulate biofilm formation, whereas phosphodiesterase (PDE) activation reduces biofilms. Respiratory secretions, which contain albumin and calcium at higher concentrations than standard growth media, promote an increase in the amount and localization of the adenylate cyclase toxin (AC), an important Bordetella virulence factor. Secreted AC present in the extracellular media or attached to the outer membrane inhibits biofilm formation. Based on these observations, we hypothesized that serum albumin and calcium inhibit biofilm formation and explored the involvement of c-di-GMP in this process. Our findings demonstrate that albumin and calcium inhibit B. bronchiseptica biofilm formation by two apparently independent mechanisms, formation, increasing with AC secretion playing a significant role, while the filamentous hemagglutinin adhesin (FHA) does not appear to be directly involved. Furthermore,and inducing c-di-GMP degradation. regulates biofilm formation in response to albumin and calcium. This study enhances our understanding of the complex mechanisms governing B. bronchiseptica biofilm formation and its modulation by host factors.
format Conjunto de datos
Conjunto de datos
author Mugni, Sabrina Laura
Ambrosis, Nicolás Martín
O'Toole, George A.
Sisti, Federico
Fernández, Julieta
author_facet Mugni, Sabrina Laura
Ambrosis, Nicolás Martín
O'Toole, George A.
Sisti, Federico
Fernández, Julieta
author_sort Mugni, Sabrina Laura
title Supplementary material for "Interplay Of Virulence Factors and Signaling Molecules: Albumin And Calcium-Mediated Biofilm Regulation In Bordetella bronchiseptia"
title_short Supplementary material for "Interplay Of Virulence Factors and Signaling Molecules: Albumin And Calcium-Mediated Biofilm Regulation In Bordetella bronchiseptia"
title_full Supplementary material for "Interplay Of Virulence Factors and Signaling Molecules: Albumin And Calcium-Mediated Biofilm Regulation In Bordetella bronchiseptia"
title_fullStr Supplementary material for "Interplay Of Virulence Factors and Signaling Molecules: Albumin And Calcium-Mediated Biofilm Regulation In Bordetella bronchiseptia"
title_full_unstemmed Supplementary material for "Interplay Of Virulence Factors and Signaling Molecules: Albumin And Calcium-Mediated Biofilm Regulation In Bordetella bronchiseptia"
title_sort supplementary material for "interplay of virulence factors and signaling molecules: albumin and calcium-mediated biofilm regulation in bordetella bronchiseptia"
publishDate 2025
url http://sedici.unlp.edu.ar/handle/10915/177239
https://doi.org/10.35537/10915/177239
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