Delivery of fluorophores by calcium phosphate-coated nanoliposomesand interaction with Staphylococcus aureus biofilms
The delivery capacity and mechanical stability of calcium phosphate (CaP) coated 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) liposomes free and adsorbed on bacterial surface was investigated introducing either acridine orange (AO) or 5,10,15,20-Tetrakis(1-methyl-4-pyridinio)porphyrin (TMP) in the aqu...
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2016
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/170193 |
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I19-R120-10915-170193 |
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I19-R120-10915-1701932024-09-14T04:08:40Z http://sedici.unlp.edu.ar/handle/10915/170193 Delivery of fluorophores by calcium phosphate-coated nanoliposomesand interaction with Staphylococcus aureus biofilms Rivero Berti, Ignacio Dell'Arciprete, María Laura Dittler, María Laura Miñán, Alejandro Guillermo Fernández Lorenzo de Mele, Mónica Alicia González, Mónica Cristina 2016 2024-09-13T18:27:18Z en Química Nanoliposomes Calcium phosphate Acridine orange 5, 10, 15, 20-Tetrakis(1-methyl-4-pyridinio)porphyrin Staphylococcus aureus The delivery capacity and mechanical stability of calcium phosphate (CaP) coated 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) liposomes free and adsorbed on bacterial surface was investigated introducing either acridine orange (AO) or 5,10,15,20-Tetrakis(1-methyl-4-pyridinio)porphyrin (TMP) in the aqueous core of the liposomes. The obtained nanomaterials were thoroughly characterized by electron and optical microscopy and by fluorescence techniques. Distribution of the AO and TMP molecules between the aqueous liposomes core and the outer solution was demonstrated by the band shifts and broadening of the excitation–emission matrices and the modified Stern-Volmer model for fluorescence quenching. In aqueous suspensions, c.a. 40% of AO was released to the outer solution while only a small percentage of TMP was observed to reach the outer liposome surface. The nanoliposomes adhesion capacity and the leaking of fluorophore molecules to Staphylococcus aureus (S. aureus) biofilms were further evaluated. A close interaction between liposomes and S. aureus biofilm was evidenced by TEM and SEM imaging. Epifluorescence experiments demonstrated that CaP-coated liposomes have good biofilm staining capability after two hours incubation of the biofilms with the liposomes, thus supporting an important release of the fluorophores when in contact with the biofilm. Altogether, the obtained results strongly suggest that CaP-coated liposomes are capable of activating drug release when in presence of S. aureus biofilms and smears. The studies herein presented, indicate that CaP-coated liposomes are potential vehicles for the selective delivery of drugs to S. aureus biofilms, as is the case of the singlet oxygen photosensitizer TMP, a well known photodynamic antibacterial agent. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 214-222 |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Química Nanoliposomes Calcium phosphate Acridine orange 5, 10, 15, 20-Tetrakis(1-methyl-4-pyridinio)porphyrin Staphylococcus aureus |
| spellingShingle |
Química Nanoliposomes Calcium phosphate Acridine orange 5, 10, 15, 20-Tetrakis(1-methyl-4-pyridinio)porphyrin Staphylococcus aureus Rivero Berti, Ignacio Dell'Arciprete, María Laura Dittler, María Laura Miñán, Alejandro Guillermo Fernández Lorenzo de Mele, Mónica Alicia González, Mónica Cristina Delivery of fluorophores by calcium phosphate-coated nanoliposomesand interaction with Staphylococcus aureus biofilms |
| topic_facet |
Química Nanoliposomes Calcium phosphate Acridine orange 5, 10, 15, 20-Tetrakis(1-methyl-4-pyridinio)porphyrin Staphylococcus aureus |
| description |
The delivery capacity and mechanical stability of calcium phosphate (CaP) coated 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) liposomes free and adsorbed on bacterial surface was investigated introducing either acridine orange (AO) or 5,10,15,20-Tetrakis(1-methyl-4-pyridinio)porphyrin (TMP) in the aqueous core of the liposomes. The obtained nanomaterials were thoroughly characterized by electron and optical microscopy and by fluorescence techniques. Distribution of the AO and TMP molecules between the aqueous liposomes core and the outer solution was demonstrated by the band shifts and broadening of the excitation–emission matrices and the modified Stern-Volmer model for fluorescence quenching. In aqueous suspensions, c.a. 40% of AO was released to the outer solution while only a small percentage of TMP was observed to reach the outer liposome surface. The nanoliposomes adhesion capacity and the leaking of fluorophore molecules to Staphylococcus aureus (S. aureus) biofilms were further evaluated. A close interaction between liposomes and S. aureus biofilm was evidenced by TEM and SEM imaging. Epifluorescence experiments demonstrated that CaP-coated liposomes have good biofilm staining capability after two hours incubation of the biofilms with the liposomes, thus supporting an important release of the fluorophores when in contact with the biofilm. Altogether, the obtained results strongly suggest that CaP-coated liposomes are capable of activating drug release when in presence of S. aureus biofilms and smears. The studies herein presented, indicate that CaP-coated liposomes are potential vehicles for the selective delivery of drugs to S. aureus biofilms, as is the case of the singlet oxygen photosensitizer TMP, a well known photodynamic antibacterial agent. |
| format |
Articulo Articulo |
| author |
Rivero Berti, Ignacio Dell'Arciprete, María Laura Dittler, María Laura Miñán, Alejandro Guillermo Fernández Lorenzo de Mele, Mónica Alicia González, Mónica Cristina |
| author_facet |
Rivero Berti, Ignacio Dell'Arciprete, María Laura Dittler, María Laura Miñán, Alejandro Guillermo Fernández Lorenzo de Mele, Mónica Alicia González, Mónica Cristina |
| author_sort |
Rivero Berti, Ignacio |
| title |
Delivery of fluorophores by calcium phosphate-coated nanoliposomesand interaction with Staphylococcus aureus biofilms |
| title_short |
Delivery of fluorophores by calcium phosphate-coated nanoliposomesand interaction with Staphylococcus aureus biofilms |
| title_full |
Delivery of fluorophores by calcium phosphate-coated nanoliposomesand interaction with Staphylococcus aureus biofilms |
| title_fullStr |
Delivery of fluorophores by calcium phosphate-coated nanoliposomesand interaction with Staphylococcus aureus biofilms |
| title_full_unstemmed |
Delivery of fluorophores by calcium phosphate-coated nanoliposomesand interaction with Staphylococcus aureus biofilms |
| title_sort |
delivery of fluorophores by calcium phosphate-coated nanoliposomesand interaction with staphylococcus aureus biofilms |
| publishDate |
2016 |
| url |
http://sedici.unlp.edu.ar/handle/10915/170193 |
| work_keys_str_mv |
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| _version_ |
1825276030210801664 |