Efecto de OPN3 y MC4R en las corrientes mediadas por KIR.1

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Encephalopsin (OPN3) is part of the opsin family of light-sensitive G-protein coupled receptors. OPN3 was discovered in deep brain regions, specifically the arcuate nucleus and the paraventricular nucleus of the hypothalamus, however there have been limited research in the functionality of OPN3. Oan...

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Autores principales: Pinto, Victoria, Raingo, Jesica
Formato: Articulo Comunicacion
Lenguaje:Español
Publicado: 2023
Materias:
Biología
patch clamp
corrientes
receptores
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/168895
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-168895
record_format dspace
spelling I19-R120-10915-1688952024-08-22T20:03:16Z http://sedici.unlp.edu.ar/handle/10915/168895 Efecto de OPN3 y MC4R en las corrientes mediadas por KIR.1 Effect of opn3 and mc4r on KIR7.1 mediated currents Pinto, Victoria Raingo, Jesica 2023-09-27 2024-08-22T13:15:33Z es Biología patch clamp corrientes receptores Encephalopsin (OPN3) is part of the opsin family of light-sensitive G-protein coupled receptors. OPN3 was discovered in deep brain regions, specifically the arcuate nucleus and the paraventricular nucleus of the hypothalamus, however there have been limited research in the functionality of OPN3. Oancea lab has discovered that OPN3 functions in a light independent manner and signals through Gai to negatively regulate the melanogenic Gαs-coupled MC1R in human epidermal melanocytes. Neuronal melanocortin receptors (MC3R and MC4R) are found in the arcuate nucleus and paraventricular nucleus of the hypothalamus and play non-redundant roles in mediating energy balance. Both MC3R and MC4R share similar structural identities to MC1R and have been found to couple to Gαs. Additionally, Cone and his colleagues found that MC4R couples to Kir7.1 in hypothalamic neurons independent of G-protein activity. Previous data in our lab has revealed that OPN3 colocalizes and forms a physical complex with MC4R. The goal of this study is to test the hypothesis that OPN3 functions to negatively modulate MC4R-mediated signaling. More specifically, we overexpress OPN3 and MC4R in HEK293 cells together with Kir7.1 and measured the potassium current at diferent potentials. We only observed a significant amount of current in cells coexpressing OPN3 and this effect seems to be occluded by MC4R addition. More experiments are required to fully understand the signaling cascade involved and what is the effect of MC4R agonists. Facultad de Ciencias Agrarias y Forestales Articulo Comunicacion http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 235-236
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Biología
patch clamp
corrientes
receptores
spellingShingle Biología
patch clamp
corrientes
receptores
Pinto, Victoria
Raingo, Jesica
Efecto de OPN3 y MC4R en las corrientes mediadas por KIR.1
topic_facet Biología
patch clamp
corrientes
receptores
description Encephalopsin (OPN3) is part of the opsin family of light-sensitive G-protein coupled receptors. OPN3 was discovered in deep brain regions, specifically the arcuate nucleus and the paraventricular nucleus of the hypothalamus, however there have been limited research in the functionality of OPN3. Oancea lab has discovered that OPN3 functions in a light independent manner and signals through Gai to negatively regulate the melanogenic Gαs-coupled MC1R in human epidermal melanocytes. Neuronal melanocortin receptors (MC3R and MC4R) are found in the arcuate nucleus and paraventricular nucleus of the hypothalamus and play non-redundant roles in mediating energy balance. Both MC3R and MC4R share similar structural identities to MC1R and have been found to couple to Gαs. Additionally, Cone and his colleagues found that MC4R couples to Kir7.1 in hypothalamic neurons independent of G-protein activity. Previous data in our lab has revealed that OPN3 colocalizes and forms a physical complex with MC4R. The goal of this study is to test the hypothesis that OPN3 functions to negatively modulate MC4R-mediated signaling. More specifically, we overexpress OPN3 and MC4R in HEK293 cells together with Kir7.1 and measured the potassium current at diferent potentials. We only observed a significant amount of current in cells coexpressing OPN3 and this effect seems to be occluded by MC4R addition. More experiments are required to fully understand the signaling cascade involved and what is the effect of MC4R agonists.
format Articulo
Comunicacion
author Pinto, Victoria
Raingo, Jesica
author_facet Pinto, Victoria
Raingo, Jesica
author_sort Pinto, Victoria
title Efecto de OPN3 y MC4R en las corrientes mediadas por KIR.1
title_short Efecto de OPN3 y MC4R en las corrientes mediadas por KIR.1
title_full Efecto de OPN3 y MC4R en las corrientes mediadas por KIR.1
title_fullStr Efecto de OPN3 y MC4R en las corrientes mediadas por KIR.1
title_full_unstemmed Efecto de OPN3 y MC4R en las corrientes mediadas por KIR.1
title_sort efecto de opn3 y mc4r en las corrientes mediadas por kir.1
publishDate 2023
url http://sedici.unlp.edu.ar/handle/10915/168895
work_keys_str_mv AT pintovictoria efectodeopn3ymc4renlascorrientesmediadasporkir1
AT raingojesica efectodeopn3ymc4renlascorrientesmediadasporkir1
AT pintovictoria effectofopn3andmc4ronkir71mediatedcurrents
AT raingojesica effectofopn3andmc4ronkir71mediatedcurrents
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