Molecular characterization of a seed-fern ovule (Pennsylvanian, Sydney Coalfield, Canada) by FTIR, <sup>13</sup>C NMR, and Py-GC-MS

Despite the abundance of detached seed-fern ovules (Euramerican and Cathaysian floral provinces, Pennsylvanian - Permian) as compression / impression, their palaeobiochemistry remains unknown. The Late Pennsylvanian strata of the Sydney Coalfield, Canada, have yielded numerous ovulate trigonocarpale...

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Detalles Bibliográficos
Autores principales: D'Angelo, José A., Werner-Zwanziger, U., Helleur, R., Zodrow, E. L.
Formato: Objeto de conferencia Resumen
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Naturales
Paleontología
Molecular characterization
Seed-fern ovule
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/16853
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-16853
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Paleontología
Molecular characterization
Seed-fern ovule
spellingShingle Ciencias Naturales
Paleontología
Molecular characterization
Seed-fern ovule
D'Angelo, José A.
Werner-Zwanziger, U.
Helleur, R.
Zodrow, E. L.
Molecular characterization of a seed-fern ovule (Pennsylvanian, Sydney Coalfield, Canada) by FTIR, <sup>13</sup>C NMR, and Py-GC-MS
topic_facet Ciencias Naturales
Paleontología
Molecular characterization
Seed-fern ovule
description Despite the abundance of detached seed-fern ovules (Euramerican and Cathaysian floral provinces, Pennsylvanian - Permian) as compression / impression, their palaeobiochemistry remains unknown. The Late Pennsylvanian strata of the Sydney Coalfield, Canada, have yielded numerous ovulate trigonocarpalean compressions, 6-8cm long, with preserved cuticles assigned to Trigonocarpus grandis (Lesquereux) Cleal et Zodrow. The macerated cuticles are analyzed by Fourier Transform Infrared spectroscopy (FTIR), Carbon-13 Nuclear Magnetic Resonance (<sup>13</sup>C NMR), and Pyrolysis Gas Chromatography-mass spectrometry (Py-GC-MS). FTIR and <sup>13</sup>C NMR data reveal a predominantly aliphatic structure including C-H groups (with or without hetero-substitution such as in alkyl and aryl alcohols, ethers, esters, and ketones). Larger CH<sub>2</sub>/CH<sub>3</sub> ratios (above 20) suggest the presence of long and straight aliphatic side chains linked to the main macromolecular structure. Other groups present include C-O (in phenols and phenoxy structures, and aryl and alkyl ethers and alcohols), C=O carbonyl groups (in carboxylic acids and conjugated and highly conjugated structures such as ketones), C=C (in aromatic carbon structures with and without bridging to other carbon groups), and O-H (in aliphatic or aromatic alcohols). In addition, <sup>13</sup>C NMR shows, compared to other cuticles in related seed ferns, a high amount of acetal groups as they occur in di- and polysaccharides. In agreement with FTIR and NMR results, Py-GC-MS data show the presence of highly aliphatic molecules (alkanes/alkenes) with carbons between C<sub>4</sub> and C<sub>8</sub> (including a complex mixture of mono and di unsaturated hydrocarbon isomers up to C<sub>8</sub>). Other compounds detected include benzene and toluene. However, the most striking chemical feature of the pyrolysates markers is the presence of 5 isomers of C<sub>5</sub>H<sub>8</sub> including isoprene (0.5 - 1.0 % abundance). The latter could be derived from some tocopherol precursor (vitamin E-like compound). If confirmed, this is the earliest chemical evidence for the presence of isoprenoids in vascular plants.
format Objeto de conferencia
Resumen
author D'Angelo, José A.
Werner-Zwanziger, U.
Helleur, R.
Zodrow, E. L.
author_facet D'Angelo, José A.
Werner-Zwanziger, U.
Helleur, R.
Zodrow, E. L.
author_sort D'Angelo, José A.
title Molecular characterization of a seed-fern ovule (Pennsylvanian, Sydney Coalfield, Canada) by FTIR, <sup>13</sup>C NMR, and Py-GC-MS
title_short Molecular characterization of a seed-fern ovule (Pennsylvanian, Sydney Coalfield, Canada) by FTIR, <sup>13</sup>C NMR, and Py-GC-MS
title_full Molecular characterization of a seed-fern ovule (Pennsylvanian, Sydney Coalfield, Canada) by FTIR, <sup>13</sup>C NMR, and Py-GC-MS
title_fullStr Molecular characterization of a seed-fern ovule (Pennsylvanian, Sydney Coalfield, Canada) by FTIR, <sup>13</sup>C NMR, and Py-GC-MS
title_full_unstemmed Molecular characterization of a seed-fern ovule (Pennsylvanian, Sydney Coalfield, Canada) by FTIR, <sup>13</sup>C NMR, and Py-GC-MS
title_sort molecular characterization of a seed-fern ovule (pennsylvanian, sydney coalfield, canada) by ftir, <sup>13</sup>c nmr, and py-gc-ms
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/16853
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