Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"

Bordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in mammals. Adhesins, toxins and secretion systems necessary for infection are regulated by the two-component system BvgAS. When the BvgAS system is inactive, there is no transcription of virulence-activated genes...

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Autores principales: Gutierrez, María de la Paz, Damron, F. Heath, Sisti, Federico, Fernández, Julieta
Formato: Conjunto de datos
Lenguaje:Español
Publicado: 2024
Materias:
Ciencias Exactas
https://purl.org/becyt/ford/1.6
Biofilm
Bordetella
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/168456
https://doi.org/10.35537/10915/168456
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-168456
record_format dspace
spelling I19-R120-10915-1684562024-08-09T18:05:22Z http://sedici.unlp.edu.ar/handle/10915/168456 https://doi.org/10.35537/10915/168456 Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica" Gutierrez, María de la Paz Damron, F. Heath Sisti, Federico Fernández, Julieta 2024-08-09T17:46:37Z 2024-08 es Ciencias Exactas https://purl.org/becyt/ford/1.6 Biofilm Bordetella Bordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in mammals. Adhesins, toxins and secretion systems necessary for infection are regulated by the two-component system BvgAS. When the BvgAS system is inactive, there is no transcription of virulence-activated genes and virulence-repressed genes (vrg) are expressed. The regulation of some vrgs in B. bronchiseptica is dependent upon the virulence-activated gene bvgR. Although having a regulatory role, no DNA-binding domain is described for BvgR. Instead, it contains an EAL domain, usually found in cyclic-di-GMP-specific phosphodiesterases (PDE). Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that regulates multiple phenotypes in bacteria, including B. bronchiseptica. The current study aimed to deepen our knowledge about BvgR. We employed RNA-seq analysis to define the BvgR regulon and then we investigated the phenotypes in which BvgR regulation might be involved such as biofilm formation, cytotoxicity, and virulence. Our result revealed that BvgR inhibits biofilm formation and flagellin expression in virulent phase. Although BvgR has long been considered a repressor protein, our results show that it also upregulates almost 100 genes. This regulation is likely indirect, as BvgR lacks a DNA-binding domain. Notably, among the upregulated genes, we identified 15 associated with the type three secretion system. Consistent with these findings, a B. bronchiseptica strain deficient in bvgR was less cytotoxic than the wild-type strain, elicited a milder immune response, and was less able to persist in the lower respiratory tract of mice. Fil: Gutierrez, María de la Paz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Sisti, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Fernández, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina Facultad de Ciencias Exactas Conjunto de datos Conjunto de datos http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/zip El material es resultado del trabajo citado. Se emplearon técnicas de identificación y cuantificación masiva de proteína (Shot gun proteomics, Orbitrap). Además, se utilizaron técnicas de microbiología clásica: determinación de formación de biofilm, movilidad, etc.
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Exactas
https://purl.org/becyt/ford/1.6
Biofilm
Bordetella
spellingShingle Ciencias Exactas
https://purl.org/becyt/ford/1.6
Biofilm
Bordetella
Gutierrez, María de la Paz
Damron, F. Heath
Sisti, Federico
Fernández, Julieta
Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
topic_facet Ciencias Exactas
https://purl.org/becyt/ford/1.6
Biofilm
Bordetella
description Bordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in mammals. Adhesins, toxins and secretion systems necessary for infection are regulated by the two-component system BvgAS. When the BvgAS system is inactive, there is no transcription of virulence-activated genes and virulence-repressed genes (vrg) are expressed. The regulation of some vrgs in B. bronchiseptica is dependent upon the virulence-activated gene bvgR. Although having a regulatory role, no DNA-binding domain is described for BvgR. Instead, it contains an EAL domain, usually found in cyclic-di-GMP-specific phosphodiesterases (PDE). Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that regulates multiple phenotypes in bacteria, including B. bronchiseptica. The current study aimed to deepen our knowledge about BvgR. We employed RNA-seq analysis to define the BvgR regulon and then we investigated the phenotypes in which BvgR regulation might be involved such as biofilm formation, cytotoxicity, and virulence. Our result revealed that BvgR inhibits biofilm formation and flagellin expression in virulent phase. Although BvgR has long been considered a repressor protein, our results show that it also upregulates almost 100 genes. This regulation is likely indirect, as BvgR lacks a DNA-binding domain. Notably, among the upregulated genes, we identified 15 associated with the type three secretion system. Consistent with these findings, a B. bronchiseptica strain deficient in bvgR was less cytotoxic than the wild-type strain, elicited a milder immune response, and was less able to persist in the lower respiratory tract of mice.
format Conjunto de datos
Conjunto de datos
author Gutierrez, María de la Paz
Damron, F. Heath
Sisti, Federico
Fernández, Julieta
author_facet Gutierrez, María de la Paz
Damron, F. Heath
Sisti, Federico
Fernández, Julieta
author_sort Gutierrez, María de la Paz
title Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title_short Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title_full Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title_fullStr Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title_full_unstemmed Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title_sort supplementary material from "bvgr is important for virulence-related phenotypes in bordetella bronchiseptica"
publishDate 2024
url http://sedici.unlp.edu.ar/handle/10915/168456
https://doi.org/10.35537/10915/168456
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