Exploring genetic diversity and variation of Ovar‑DRB1 gene in Sudan Desert Sheep using targeted next‑generation sequencing

Introduction The Ovar-DRB1 gene, a crucial element of the Major Histocompatibility Complex (MHC) Class II region, initiates adaptive immunity by presenting antigens to T-cells. Genetic diversity in sheep, particularly in MHC Class II genes like Ovar-DRB1, directly influences the specturm of presente...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Salim, Bashir, Nakao, Ryo, Chatanga, Elisha, Marcuzzi, Olivia, Eissawi, Muna Ahmed, Almathen, Faisal, Hanotte, Olivier, Giovambattista, Guillermo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2024
Materias:
Ciencias Veterinarias
Ovar-DRB1 gene
Next-generation sequencing
Genetic diversity
Sudan Desert Sheep
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/167728
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-167728
record_format dspace
spelling I19-R120-10915-1677282024-07-03T20:10:26Z http://sedici.unlp.edu.ar/handle/10915/167728 Exploring genetic diversity and variation of Ovar‑DRB1 gene in Sudan Desert Sheep using targeted next‑generation sequencing Salim, Bashir Nakao, Ryo Chatanga, Elisha Marcuzzi, Olivia Eissawi, Muna Ahmed Almathen, Faisal Hanotte, Olivier Giovambattista, Guillermo 2024 2024-07-03T15:11:49Z en Ciencias Veterinarias Ovar-DRB1 gene Next-generation sequencing Genetic diversity Sudan Desert Sheep Introduction The Ovar-DRB1 gene, a crucial element of the Major Histocompatibility Complex (MHC) Class II region, initiates adaptive immunity by presenting antigens to T-cells. Genetic diversity in sheep, particularly in MHC Class II genes like Ovar-DRB1, directly influences the specturm of presented antigens impacting immune responses and disease susceptability. Understanding the allelic diversity of Ovar-DRB1 gene in Sudan Desert Sheep (SDS) is essential for uncovering the genetic basis of immune responses and disease resistance, given the the breeds significance in Sudan’s unique environment. Methods Utilizing Targeted Next-Generation Sequencing (NGS) we explore allelic diversity in Ovar-DRB1 gene within SDS. Successfully ampliying and and sequencing the second exon of this gene in 288 SDS samples representing six breeds provided a comprehensive allelic profile, enabling a detalied examination of the gene’s genetic makeup. Results We identifed forty-six alleles, including four previously unreported, enrichness the genetic diversity of SDS breeds. These alleles exhibiting non-uniform distribution, varying frequencies across breeds, indicating a breed-specific genetic landscape. Certain alleles, known and novel, show higher frequencies in specific populations, suggesting potential associations with adaptive immune responses. Identifying these alleles sets the stage for investigating their functional roles and implications for disease resistance. Genetic differentiation among SDS breeds, as indicated by FST values and clustering analyses, highlights a unique genetic makeup shaped by geographic and historical factors. These differentiation patterns among SDS breeds have broader implications for breed conservation and targeted breeding to enhance disease resistance in specific populations. Conclusion This study unveils Ovar-DRB1 gene allelic diversity in SDS breeds through targeted NGS and genetic analyses, revealing new alleles that underscore the breeds’ unique genetic profile. Insights into the genetic factors governing immune responses and disease resistance emerge, promising for optimization of breeding strategies for enhanced livestock health in Sudan’s unique environment. Instituto de Genética Veterinaria Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Ovar-DRB1 gene
Next-generation sequencing
Genetic diversity
Sudan Desert Sheep
spellingShingle Ciencias Veterinarias
Ovar-DRB1 gene
Next-generation sequencing
Genetic diversity
Sudan Desert Sheep
Salim, Bashir
Nakao, Ryo
Chatanga, Elisha
Marcuzzi, Olivia
Eissawi, Muna Ahmed
Almathen, Faisal
Hanotte, Olivier
Giovambattista, Guillermo
Exploring genetic diversity and variation of Ovar‑DRB1 gene in Sudan Desert Sheep using targeted next‑generation sequencing
topic_facet Ciencias Veterinarias
Ovar-DRB1 gene
Next-generation sequencing
Genetic diversity
Sudan Desert Sheep
description Introduction The Ovar-DRB1 gene, a crucial element of the Major Histocompatibility Complex (MHC) Class II region, initiates adaptive immunity by presenting antigens to T-cells. Genetic diversity in sheep, particularly in MHC Class II genes like Ovar-DRB1, directly influences the specturm of presented antigens impacting immune responses and disease susceptability. Understanding the allelic diversity of Ovar-DRB1 gene in Sudan Desert Sheep (SDS) is essential for uncovering the genetic basis of immune responses and disease resistance, given the the breeds significance in Sudan’s unique environment. Methods Utilizing Targeted Next-Generation Sequencing (NGS) we explore allelic diversity in Ovar-DRB1 gene within SDS. Successfully ampliying and and sequencing the second exon of this gene in 288 SDS samples representing six breeds provided a comprehensive allelic profile, enabling a detalied examination of the gene’s genetic makeup. Results We identifed forty-six alleles, including four previously unreported, enrichness the genetic diversity of SDS breeds. These alleles exhibiting non-uniform distribution, varying frequencies across breeds, indicating a breed-specific genetic landscape. Certain alleles, known and novel, show higher frequencies in specific populations, suggesting potential associations with adaptive immune responses. Identifying these alleles sets the stage for investigating their functional roles and implications for disease resistance. Genetic differentiation among SDS breeds, as indicated by FST values and clustering analyses, highlights a unique genetic makeup shaped by geographic and historical factors. These differentiation patterns among SDS breeds have broader implications for breed conservation and targeted breeding to enhance disease resistance in specific populations. Conclusion This study unveils Ovar-DRB1 gene allelic diversity in SDS breeds through targeted NGS and genetic analyses, revealing new alleles that underscore the breeds’ unique genetic profile. Insights into the genetic factors governing immune responses and disease resistance emerge, promising for optimization of breeding strategies for enhanced livestock health in Sudan’s unique environment.
format Articulo
Articulo
author Salim, Bashir
Nakao, Ryo
Chatanga, Elisha
Marcuzzi, Olivia
Eissawi, Muna Ahmed
Almathen, Faisal
Hanotte, Olivier
Giovambattista, Guillermo
author_facet Salim, Bashir
Nakao, Ryo
Chatanga, Elisha
Marcuzzi, Olivia
Eissawi, Muna Ahmed
Almathen, Faisal
Hanotte, Olivier
Giovambattista, Guillermo
author_sort Salim, Bashir
title Exploring genetic diversity and variation of Ovar‑DRB1 gene in Sudan Desert Sheep using targeted next‑generation sequencing
title_short Exploring genetic diversity and variation of Ovar‑DRB1 gene in Sudan Desert Sheep using targeted next‑generation sequencing
title_full Exploring genetic diversity and variation of Ovar‑DRB1 gene in Sudan Desert Sheep using targeted next‑generation sequencing
title_fullStr Exploring genetic diversity and variation of Ovar‑DRB1 gene in Sudan Desert Sheep using targeted next‑generation sequencing
title_full_unstemmed Exploring genetic diversity and variation of Ovar‑DRB1 gene in Sudan Desert Sheep using targeted next‑generation sequencing
title_sort exploring genetic diversity and variation of ovar‑drb1 gene in sudan desert sheep using targeted next‑generation sequencing
publishDate 2024
url http://sedici.unlp.edu.ar/handle/10915/167728
work_keys_str_mv AT salimbashir exploringgeneticdiversityandvariationofovardrb1geneinsudandesertsheepusingtargetednextgenerationsequencing
AT nakaoryo exploringgeneticdiversityandvariationofovardrb1geneinsudandesertsheepusingtargetednextgenerationsequencing
AT chatangaelisha exploringgeneticdiversityandvariationofovardrb1geneinsudandesertsheepusingtargetednextgenerationsequencing
AT marcuzziolivia exploringgeneticdiversityandvariationofovardrb1geneinsudandesertsheepusingtargetednextgenerationsequencing
AT eissawimunaahmed exploringgeneticdiversityandvariationofovardrb1geneinsudandesertsheepusingtargetednextgenerationsequencing
AT almathenfaisal exploringgeneticdiversityandvariationofovardrb1geneinsudandesertsheepusingtargetednextgenerationsequencing
AT hanotteolivier exploringgeneticdiversityandvariationofovardrb1geneinsudandesertsheepusingtargetednextgenerationsequencing
AT giovambattistaguillermo exploringgeneticdiversityandvariationofovardrb1geneinsudandesertsheepusingtargetednextgenerationsequencing
_version_ 1807223555389128704

Ejemplares similares