Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions

Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship...

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Detalles Bibliográficos
Autores principales: Gambaro, Sabrina Eliana, Zubiría, María Guillermina, Giordano, Alejandra, Castro, Patricia F., Garraza, Carolina, Harnichar, Alejandro Ezequiel, Alzamendi, Ana, Spinedi, Eduardo Julio, Giovambattista, Andrés
Formato: Articulo
Lenguaje:Inglés
Publicado: 2024
Materias:
Biología
Spexin
thermogenesis
beige adipocytes
white adipose tissue
UCP1
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/167624
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-167624
record_format dspace
spelling I19-R120-10915-1676242024-07-01T20:09:34Z http://sedici.unlp.edu.ar/handle/10915/167624 Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions Gambaro, Sabrina Eliana Zubiría, María Guillermina Giordano, Alejandra Castro, Patricia F. Garraza, Carolina Harnichar, Alejandro Ezequiel Alzamendi, Ana Spinedi, Eduardo Julio Giovambattista, Andrés 2024 2024-07-01T13:51:08Z en Biología Spexin thermogenesis beige adipocytes white adipose tissue UCP1 Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation. Instituto Multidisciplinario de Biología Celular Centro de Endocrinología Experimental y Aplicada Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Spexin
thermogenesis
beige adipocytes
white adipose tissue
UCP1
spellingShingle Biología
Spexin
thermogenesis
beige adipocytes
white adipose tissue
UCP1
Gambaro, Sabrina Eliana
Zubiría, María Guillermina
Giordano, Alejandra
Castro, Patricia F.
Garraza, Carolina
Harnichar, Alejandro Ezequiel
Alzamendi, Ana
Spinedi, Eduardo Julio
Giovambattista, Andrés
Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
topic_facet Biología
Spexin
thermogenesis
beige adipocytes
white adipose tissue
UCP1
description Spexin (SPX) is a novel adipokine that plays an emerging role in metabolic diseases due to its involvement in carbohydrate homeostasis, weight loss, appetite control, and gastrointestinal movement, among others. In obese patients, SPX plasma levels are reduced. Little is known about the relationship between SPX and white adipose tissue (WAT) thermogenesis. Therefore, the aim of the present study was to evaluate the role of SPX in this process. C57BL/6J male mice were treated or not with SPX for ten days. On day 3, mice were randomly divided into two groups: one kept at room temperature and the other kept at cold temperature (4 ◦C). Caloric intake and body weight were recorded daily. At the end of the protocol, plasma, abdominal (epididymal), subcutaneous (inguinal), and brown AT (EAT, IAT, and BAT, respectively) depots were collected for measurements. We found that SPX treatment reduced Uncoupling protein 1 levels inWAT under both basal and cold conditions. SPX also reduced cox8b and pgc1α mRNA levels and mitochondrial DNA, principally in IAT. SPX did not modulate the number of beige precursors. SPX decreased spx levels in IAT depots and galr2 in WAT depots. No differences were observed in the BAT depots. In conclusion, we showed, for the first time, that SPX treatment in vivo reduced the thermogenic process in subcutaneous and abdominal AT, being more evident under cold stimulation.
format Articulo
Articulo
author Gambaro, Sabrina Eliana
Zubiría, María Guillermina
Giordano, Alejandra
Castro, Patricia F.
Garraza, Carolina
Harnichar, Alejandro Ezequiel
Alzamendi, Ana
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_facet Gambaro, Sabrina Eliana
Zubiría, María Guillermina
Giordano, Alejandra
Castro, Patricia F.
Garraza, Carolina
Harnichar, Alejandro Ezequiel
Alzamendi, Ana
Spinedi, Eduardo Julio
Giovambattista, Andrés
author_sort Gambaro, Sabrina Eliana
title Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title_short Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title_full Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title_fullStr Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title_full_unstemmed Role of Spexin in White Adipose Tissue Thermogenesis under Basal and Cold-Stimulated Conditions
title_sort role of spexin in white adipose tissue thermogenesis under basal and cold-stimulated conditions
publishDate 2024
url http://sedici.unlp.edu.ar/handle/10915/167624
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